Integration of trehalose-6-phosphate signaling by FAF proteins in Arabidopsis thaliana

拟南芥中 FAF 蛋白整合海藻糖-6-磷酸信号传导

• 批准号: 49804230
• 负责人: Professor Dr. Markus Schmid
• 金额: --
• 依托单位: Department of Plant Physiology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/49804230?language=en
• 关键词: Integration; trehalose; phosphate; signaling; FAF

Plants produce new organs throughout their lives from groups of pluripotent stem cells called meristems, located at the tips of the shoot and the root. The size of the shoot meristem is tightly controlled by a feedback loop, which involves the homeodomain transcription factor WUSCHEL (WUS) and the CLAVATA (CLV) genes. This regulatory circuit is further fine-tuned by the sugars sucrose and trehalose. We have identified a family of four related plant-specific proteins, encoded by the FANTASTIC FOUR (FAF) genes, that can modulate shoot meristem size in Arabidopsis. Interestingly, the FAF proteins interact with TPS1, an enzyme that synthesizes trehalose-6- phosophate, a precursor of the disaccharide trehalose. Here we propose a series of experiments to investigate the molecular function of the FAF proteins in meristem and growth control. We also seek to follow up on our data indicating the regulation of the FAF proteins by BPM proteins. Finally, we will analyze how the FAF proteins modulate TPS1 function to affect trehalose homeostasis and the effect of these trehalose metabolizing proteins on development. In addition to offering a comprehensive analysis of an interesting and novel protein family, the proposed work will enhance our knowledge of how metabolites, in this case trehalose and its precursor trehalose-6-phosphate, contribute to development and growth.

植物在其一生中通过位于芽和根尖端的称为分生组织的多能干细胞群产生新器官。芽分生组织的大小受到反馈环的严格控制，该反馈环涉及同源域转录因子 WUSCHEL (WUS) 和 CLAVATA (CLV) 基因。该调节回路由蔗糖和海藻糖进一步微调。我们已经鉴定出由神奇四侠 (FAF) 基因编码的四种相关植物特异性蛋白家族，它们可以调节拟南芥中芽分生组织的大小。有趣的是，FAF 蛋白与 TPS1 相互作用，TPS1 是一种合成海藻糖 6 磷酸酯（二糖海藻糖的前体）的酶。在这里，我们提出了一系列实验来研究 FAF 蛋白在分生组织和生长控制中的分子功能。我们还寻求跟进我们的数据，表明 BPM 蛋白对 FAF 蛋白的调节。最后，我们将分析FAF蛋白如何调节TPS1功能以影响海藻糖稳态以及这些海藻糖代谢蛋白对发育的影响。除了对一个有趣且新颖的蛋白质家族进行全面分析之外，拟议的工作还将增强我们对代谢物（在本例中为海藻糖及其前体海藻糖-6-磷酸）如何促进发育和生长的了解。