Dynamics of signal transduction in the system of organogenesis and regeneration

器官发生和再生系统中信号转导的动力学

基本信息

批准号：
13044003
负责人：
UENO Naoto
金额：
$ 250.3万
依托单位：
National Institute for Basic Biology (2004-2005)Okazaki National Research Institutes (2001-2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2005
项目状态：
已结题

项目摘要

We performed a series of investigations involving systematic and comprehensive studies using model animals, to establish a platform for clarifying the machinery that controls early development consisting of complex cell-to- cell interactions "Early developmental systems" which we call and the relationship among their molecular components. We also carried out functional analyses of components (genes and proteins) identified by the analyses to ask their in vivo role in early embryogenesis. By the coordination of Naoto Ueno, Masanori Taira, and Kosuke Tashiro for using Xenopus laevis as a model system, construction of cDNA libraries, determination of nucleotide sequence of the cDNAs, fabrication of high-density microarray, comprehensive analyses of gene expression by the microarray during early development, spatial and temporal gene expression analysis, and construction of databases containing those information were achieved. Particularly, Ueno and Tashiro optimized the combined methodology of the high-density microarray and overexpression of polypeptide growth factors in embryos. Through the analyses which we established, Ueno and Taira focused on the regulation of gastrulation and head development, respectively, and investigate the function of the identified several genes essential for those developmental events. Satoru Kobayashi established a method of labeling germ cells specifically by expressing GFP under the regulation of a germ cell-specific promoter and germ cells were selected by cell- sorting. The microarray analysis of germ cell specific genes identified a number of genes implicated in germ cell differentiation, maintenance, and migration. It is noteworthy that a large group of genes that are predicted to be involved in one particular function was identified. Taken together these achievements prove that systematic and comprehensive analyses of genes are very effective to clarify the molecular network of early developmental processes.

我们进行了一系列研究，涉及使用模型动物的系统和全面研究，以建立一个平台，以阐明控制早期发育的机械，该机械由复杂的细胞相互作用组成的“早期发育系统”组成，我们称之为我们称之为它们的分子成分之间的关系。我们还通过分析鉴定出其在早期胚胎发生中的体内作用的成分（基因和蛋白质）的功能分析。 By the coordination of Naoto Ueno, Masanori Taira, and Kosuke Tashiro for using Xenopus laevis as a model system, construction of cDNA libraries, determination of nucleotide sequence of the cDNAs, fabrication of high-density microarray, comprehensive analyses of gene expression by the microarray during early development, spatial and temporal gene expression analysis, and construction of databases containing those information were成就了。特别是，Ueno和Tashiro优化了胚胎中高密度微阵列和多肽生长因子的过表达的组合方法。通过我们建立的分析，UENO和TAIRA分别侧重于胃和头部发育的调节，并研究了这些发育事件必不可少的几个基因的功能。 Satoru Kobayashi通过细胞分类选择了在生殖细胞特异性启动子调节下通过调节生殖细胞特异性启动子调节的GFP特异性标记生殖细胞的方法。生殖细胞特异性基因的微阵列分析确定了与生殖细胞分化，维持和迁移有关的许多基因。值得注意的是，发现一大批被预测参与一个特定功能的基因。综上所述，这些成就证明了基因的系统和全面分析对于阐明早期发育过程的分子网络非常有效。