Molecular links between vector transport machinery and cell adhesion machinery

载体运输机械和细胞粘附机械之间的分子联系

基本信息

批准号：
12144203
负责人：
HATA Yutaka
金额：
$ 32.64万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2004
项目状态：
已结题

项目摘要

In this project, we have analyzed the molecular links between vector transport machinery and cell adhesion machinery. Our primary focus is on membrane-associated proteins that interact directly or indirectly with cell adhesion molecules.We identified novel membrane-associated proteins including PAPIN, ankycorbin, and carom. PAPIN is a multi-PDZ protein and presumably a scaffold protein. Both of ankycorbin and carom indirectly interact with actin. Interestingly, carom interacts with MAGI-1, a tight junction-associated protein, before cell junctions become mature but it comes to bind to CASK, a different membrane-associated protein, in fully polarized epithelial cells. We also clarified how membrane-associated proteins are targeted to epithelial junctions. MAGI-1 is recruited to the lateral membranes through the interaction with beta-catenin. PAPIN and ERBIN interact with p0071, a member of catenin family proteins, but their subcellular localizations are independent of these interactions … More . Likewise, Lin-7, a beta-catenin-interactor, is recruited to the lateral membranes by a cell adhesion molecule, nectin, and its associated protein, afadin, but not by beta-catenin. We also discovered a novel cell adhesion molecule that binds to MAGI-1 and named it JAM4. JAM4 and MAGI-1 co-operate to regulate the intercellular permeability. JAM4 enhances hepatocyte growth factor-mediated signaling putatively by activating Rac. At synapses, a neuronal isoform of MAGI-1 named S-SCAM, which is recruited by beta-catenin to cadherin-based adhesion sites, subsequently induces another cell adhesion mediated by neuroligin and triggers the remodeling of actin cytoskeleton. Similarly, in epithelial cells, nectin induces the cadherin-based adhesion via afadin and the accumulation ofJAM1 that is a component of tight junctions. Moreover, we have found that MAGI-1 links JAM4 and nephrin in kidney glomeruli. All these findings indicate that membrane-associated proteins are important to link different categories of cell adhesion molecules. Finally, we have obtained four novel proteins binding to microtubules. Less

在该项目中，载体机械和细胞粘附机制之间的分子连接是蛋白质的蛋白质与紧密相关的蛋白质Magi-1相互作用，在细胞连接变成成熟的屁股之前，也与木桶结合，也将与clanbrane相关的蛋白质与上皮结合到上皮交界处。 -Catenin和Erbin与P0071相互作用，P0071是Catenin家族蛋白的成员，但它们的亚细胞位置是Thenteractions的独立性...更多。细胞粘附分子及其相关的蛋白质Afadin，而不是通过β-catenin，我们还发现了粘附的分子耐受性的耐受性肝细胞生长因子介导的信号通过Beta-cateninin在基于钙粘着蛋白的粘附状态下招募的RAC诱导了另一种和神经素介导的hishion和触发肌动蛋白细胞骨架的重塑。我们发现肾肾小球中的MAGI-1 JAM4和肾素都发现，膜相关的蛋白是将不同类别的细胞倾向分子链接起来的蛋白质。

项目成果

期刊论文数量（164）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Association of ASIP/mPAR-3 with adherens junctions of mouse neuroepithelial cells.

ASIP/mPAR-3 与小鼠神经上皮细胞粘附连接的关联。

DOI：
发表时间：
2002
期刊：
Dev Dyn 225
影响因子：
0
作者：
Takai Y;Nakanishi H;Nishimura W et al.;Iida J et al.;Yao I et al.;Ohtsuka T et al.;Kim Y et al.;Momose Y et al.;Yamamoto Y et al.;Peng Y-E et al.;Ozaki-Kuroda K et al.;Hirabayashi S et al.;Ohno H et al.;Manabe N et al.
通讯作者：
Manabe N et al.

Roles of immunoglobulin-like loops of junctional cell adhesion molecule 4; Involvement in the subcellular localization and the cell adhesion.

免疫球蛋白样环的连接细胞粘附分子4的作用；

DOI：
发表时间：
2003
期刊：
Genes Cells 8
影响因子：
0
作者：
Tajima M et al.
通讯作者：
Tajima M et al.

MAGI-1 is a component of the glomerulart slit diaphragm that is tightly associated with nephrin.

MAGI-1 是肾小球裂隙隔膜的一个组成部分，与去氧肾上腺素紧密相关。

DOI：
发表时间：
2005
期刊：
Lav Invest 85
影响因子：
0
作者：
Hirabayashi S et al.
通讯作者：
Hirabayashi S et al.

Role of he second immunoglobulin-like loop of nectin in cell-cell adhesion.

连接蛋白的第二个免疫球蛋白样环在细胞-细胞粘附中的作用。

DOI：
发表时间：
2002
期刊：
Biochem Biophys Res Commun 293
影响因子：
0
作者：
Takai Y;Nakanishi H;Nishimura W et al.;Iida J et al.;Yao I et al.;Ohtsuka T et al.;Kim Y et al.;Momose Y et al.;Yamamoto Y et al.;Peng Y-E et al.;Ozaki-Kuroda K et al.;Hirabayashi S et al.;Ohno H et al.;Manabe N et al.;Fukuhara A et al.;Fukuhara A et al.;Ohtakara K et al.;Tanaka Y et al.;Momose Y et al.
通讯作者：
Momose Y et al.

Tajima, M., et al.: "Roles of immunoglobulin-like loops of junctional cell adhesion molecule 4 ; involvement in the subcellular localization and the cell adhesion."Genes to Cells. 8(9). 759-768 (2003)

Tajima, M., 等人：“连接细胞粘附分子 4 的免疫球蛋白样环的作用；参与亚细胞定位和细胞粘附。”基因到细胞。