Purification and ampliefication of hematopoietic stem cells

造血干细胞的纯化和扩增

基本信息

批准号：
06277104
负责人：
SUDA Toshio
金额：
$ 128.83万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1998
项目状态：
已结题

项目摘要

Soda, Miura and Kanakura have shown that some signal transduction pathways in hemopoictic stem cells (HSC). Novel receptor tyrosine kinases such as TIEs, HTK and STK/RON were cloned from purified HSC by RT-PCR and especially TIE-1 and 2 are expressed in hematopotetic stem cells as well as in vascular endothelial cells From these findings, it becomes possible to analyze the development of hematopoietic stem cells from angioblasts, which are endothelial precursor cells. It was clarified that gain of function of c Kit caused the autonomous proliferation of hematopoietic cells (mast cells) and specified cells in the gut (Kajal cells).Nakauchi has demonstrated that CD34tィイD1+ィエD1KitィイD1+ィエD1ScaィイD1+ィエD1LinィイD1-ィエD1 and CD34ィイD1+ィエD1KitィイD1+ィエD1ScaィイD1+ィエD1LinィイD1-ィエD1 cells of adult murine bone marrow have been shown to contain long-and short-term repopulating hematopoictic stem cells by a transplantation assay. CD34 negative stein cells are also confirmed in human hematopoletic system. There has been great interest in the ex vivo expansion of human hemopoictic stem/progenitor cells for clinical applications. Nakahata has shown that heanatopoietic progenitor cellscan be expanded in the presence of soluble IL-6 receptor+IL-6+SCF. However. stein cell expansion is still insatisfactoiy. Asano has tried to establish monkey transplantation system to analyze the hurtan HSC.Yamamoto has clearly shown that GATA and Maf families am involved in erythropoiesis by the GATA-1 KO mice and GATA promoter-LacZ transgenic mice.

Soda、Miura 和 Kanakura 表明，通过 RT-PCR 从纯化的 HSC 中克隆了造血干细胞 (HSC) 中的一些新型受体酪氨酸激酶，例如 TIE、HTK 和 STK/RON，尤其是 TIE-1 和 2。在造血干细胞以及血管内皮细胞中表达根据这些发现，可以分析造血干细胞的发育血管母细胞是内皮前体细胞，已明确 C Kit 的功能获得导致造血细胞（肥大细胞）和肠道中的特定细胞（Kajal 细胞）的自主增殖。Nakauchi 已证明 CD34T-D1+ D1Kit D1+。 D1Sca D1+D1Lin D1-D1 和通过移植试验，已证实成年小鼠骨髓中的 CD34D1+D1KitD1+D1ScaD1+D1LinD1-D1 细胞含有长期和短期再生造血干细胞，在人类造血系统中也已得到证实。对人类造血细胞的离体扩增非常感兴趣然而，用于临床应用的干/祖细胞。中畑已经证明造血祖细胞可以在可溶性IL-6受体+IL-6+SCF的存在下扩增。浅野试图建立猴移植系统，但斯坦细胞扩增仍然不令人满意。分析hurtan HSC。Yamamoto已经清楚地表明GATA和Maf家族参与GATA-1 KO小鼠和GATA的红细胞生成启动子-LacZ 转基因小鼠。