Exploring the impact of Xanthomonas euvesicatoria effector XopL on ABA signaling and host cell metabolism.

探索 Xanthomonas euvesicatoria 效应子 XopL 对 ABA 信号传导和宿主细胞代谢的影响。

• 批准号: 492635023
• 负责人: Dr. Jessica Erickson, Ph.D.
• 金额: --
• 依托单位: Abteilung Biochemie pflanzlicher Interaktionen
• 依托单位国家: 德国
• 项目类别: WBP Position
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/492635023?language=en
• 关键词: Exploring; impact; Xanthomonas; euvesicatoria; effector

Xanthomonads are a genus of Gram-negative phytopathogens that cause disease in hundreds of economically important crop species worldwide. Xanthomonads are hemibiotrophic pathogens which spend a disproportionate amount of their life cycle living in/feeding off living plant tissue (biotrophic phase), but ultimately cause cell death during later stages of the infection (necrotrophic phase). Biotrophic growth phases require constant subversion of the plant immune system, and simultaneous conversion of the plant cell apoplast into a habitable environment. To address these challenges Xanthomonads utilize the type-III secretion system (T3SS) to inject bacterial-derived type III-secreted effector proteins (T3Es) directly into the host cytosol. T3Es are essential to pathogen virulence and it is generally understood that once inside, T3Es manipulate plant cell processes to support life in the apoplast. Intensive research into effector targets have revealed many of the plant processes that are vulnerable to pathogen attack, but exactly how these effector functions translate to changes in the apoplast where Xanthomonas actually resides are generally understudied. Going forward an understanding of Xanthomonas virulence requires that we link T3E function inside the cell to outcomes for the bacteria in the intracellular space.X. euvesicatoria (Xe) strain 85-10, the causal agent of bacterial spot disease on pepper and tomato, secretes 36 effector proteins, many of which are termed Xanthomonas outer proteins (Xops). One such protein, XopL, displays E3 ligase function in plants, where it hijacks the plant ubiquitylation cascade to modify plant substrate proteins. Preliminary work on XopL suggests that it might manipulate host cell metabolism by targeting a known ABA signaling component, ARIA (ARM REPEAT PROTEIN INTERACTING WITH ABF), for degradation. ARIA regulates the expression of genes important for the production of primary and secondary metabolites known to influence the success of pathogen growth in the apoplast. The goal of the proposed project is to utilize the XopL-ARIA interaction as a case study to determine whether the manipulation of the ABA signal cascade inside the cell can be linked to changes in the apoplast.

黄单胞菌是革兰氏阴性植物病原体的一属，可导致全球数百种重要经济作物物种患病。黄单胞菌是半生物营养型病原体，其生命周期的大部分时间生活在活植物组织中/以活植物组织为食（生物营养阶段），但最终在感染的后期阶段（坏死营养阶段）导致细胞死亡。生物营养生长期需要不断破坏植物免疫系统，并同时将植物细胞质外体转化为适宜居住的环境。为了应对这些挑战，黄单胞菌利用 III 型分泌系统 (T3SS) 将细菌衍生的 III 型分泌效应蛋白 (T3E) 直接注射到宿主细胞质中。 T3E 对于病原体的毒力至关重要，人们普遍认为，一旦进入内部，T3E 就会操纵植物细胞过程以支持质外体中的生命。对效应子靶点的深入研究揭示了许多容易受到病原体攻击的植物过程，但这些效应子功能如何转化为黄单胞菌实际居住的质外体的变化，人们普遍还没有充分研究。为了进一步了解黄单胞菌毒力，我们需要将细胞内的 T3E 功能与细胞内空间细菌的结果联系起来。 euvesicatoria (Xe) 菌株 85-10 是辣椒和番茄细菌性斑点病的病原体，分泌 36 种效应蛋白，其中许多被称为黄单胞菌外部蛋白 (Xops)。其中一种蛋白质 XopL 在植物中表现出 E3 连接酶功能，它劫持植物泛素化级联以修饰植物底物蛋白。 XopL 的初步研究表明，它可能通过靶向已知的 ABA 信号成分 ARIA（与 ABF 相互作用的臂重复蛋白）进行降解来操纵宿主细胞代谢。 ARIA 调节对初级和次级代谢物的产生很重要的基因的表达，这些代谢物已知会影响病原体在质外体中生长的成功。该项目的目标是利用 XopL-ARIA 相互作用作为案例研究，以确定细胞内 ABA 信号级联的操纵是否与质外体的变化有关。