Exploring the impact of Xanthomonas euvesicatoria effector XopL on ABA signaling and host cell metabolism.

探索 Xanthomonas euvesicatoria 效应子 XopL 对 ABA 信号传导和宿主细胞代谢的影响。

• 批准号: 492635023
• 负责人: Dr. Jessica Erickson, Ph.D.
• 金额: --
• 依托单位: Abteilung Biochemie pflanzlicher Interaktionen
• 依托单位国家: 德国
• 项目类别: WBP Position
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/492635023?language=en
• 关键词: Exploring; impact; Xanthomonas; euvesicatoria; effector

Xanthomonads are a genus of Gram-negative phytopathogens that cause disease in hundreds of economically important crop species worldwide. Xanthomonads are hemibiotrophic pathogens which spend a disproportionate amount of their life cycle living in/feeding off living plant tissue (biotrophic phase), but ultimately cause cell death during later stages of the infection (necrotrophic phase). Biotrophic growth phases require constant subversion of the plant immune system, and simultaneous conversion of the plant cell apoplast into a habitable environment. To address these challenges Xanthomonads utilize the type-III secretion system (T3SS) to inject bacterial-derived type III-secreted effector proteins (T3Es) directly into the host cytosol. T3Es are essential to pathogen virulence and it is generally understood that once inside, T3Es manipulate plant cell processes to support life in the apoplast. Intensive research into effector targets have revealed many of the plant processes that are vulnerable to pathogen attack, but exactly how these effector functions translate to changes in the apoplast where Xanthomonas actually resides are generally understudied. Going forward an understanding of Xanthomonas virulence requires that we link T3E function inside the cell to outcomes for the bacteria in the intracellular space.X. euvesicatoria (Xe) strain 85-10, the causal agent of bacterial spot disease on pepper and tomato, secretes 36 effector proteins, many of which are termed Xanthomonas outer proteins (Xops). One such protein, XopL, displays E3 ligase function in plants, where it hijacks the plant ubiquitylation cascade to modify plant substrate proteins. Preliminary work on XopL suggests that it might manipulate host cell metabolism by targeting a known ABA signaling component, ARIA (ARM REPEAT PROTEIN INTERACTING WITH ABF), for degradation. ARIA regulates the expression of genes important for the production of primary and secondary metabolites known to influence the success of pathogen growth in the apoplast. The goal of the proposed project is to utilize the XopL-ARIA interaction as a case study to determine whether the manipulation of the ABA signal cascade inside the cell can be linked to changes in the apoplast.

黄胞菌是革兰氏阴性植物病的属，在全球数百种具有经济上重要的农作物物种中引起疾病​​。黄胞菌是半生物营养病原体，其生命周期的花费不成比例，以居住/喂养活植物组织（生物营养期），但最终在感染后期导致细胞死亡（坏死性阶段）。生物营养阶段需要持续不断地颠覆植物免疫系统，并同时将植物细胞凋亡的转化为可居住的环境。为了应对这些挑战，黄金瘤利用III型分泌系统（T3SS）将细菌衍生的III型效应蛋白（T3ES）直接直接进入宿主细胞质中。 T3ES对于病原体毒力至关重要，通常可以理解，一旦内部，T3ES操纵植物细胞过程以支持凋亡中的生命。对效应靶标的深入研究揭示了许多容易受到病原体攻击的植物过程，但是这些效应子的功能如何转化为Xanthomonas实际居住的质体的变化。未来的了解Xanthomonas毒力要求我们将细胞内的T3E功能与细胞内空间中细菌的结果联系起来。 Euvesicatoria（XE）菌株85-10，辣椒和番茄上细菌斑点疾病的因果剂，分泌36个效应蛋白，其中许多被称为Xanthomonas外蛋白（XOPS）。一种这样的蛋白质XOPL显示出植物中的E3连接酶功能，在那里它劫持了植物泛素化级联反应以修饰植物底物蛋白。 XOPL的初步工作表明，它可能通过靶向已知的ABA信号成分ARIA（ARM重复蛋白与ABF相互作用）来操纵宿主细胞代谢，以降解。 ARIA调节基因的表达对于生产一级和继发代谢产物的生产至关重要，已知会影响凋亡中病原体生长的成功。拟议项目的目的是利用XOPL-ARIA相互作用作为案例研究，以确定是否可以将细胞内的ABA信号级联反应操作与凋亡的变化有关。