Changes of Full-field and Multifocal Electroretinograms Following Damage of the Optic Nerve

视神经损伤后全视野和多焦视网膜电图的变化

基本信息

批准号：
17591850
负责人：
MACHIDA Shigeki
金额：
$ 2.24万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591850/
关键词：
electoretinogram ERG inner retina retinal ganglion cell glaucoma diabetic retinopathy macular hole photoreceptor 視神経障害 photopic negative response 多局所網膜電図錐体ERG

项目摘要

A. Results based on animal experiments1. ERG changes following the optic nerve damageAfter the optic nerve was impaired by applying diathermy around the optic nerve head in cats, we investigated ERG changes with time. The s-wave of the mfERG disappeared 1 week after the damage. On the other hand, the photopic negative response (PhNR) significantly deteriorated 1 month after the damage. This indicates that the mfERG detects functional changes earlier than the full-field ERG after the optic nerve damage.2. Functional changes of the inner retina associated with photoreceptor degenerationWe found that an interesting change of the inner retinal function in rats with inherited photoreceptor degeneration. We recorded ERGs from Royal College of Surgeons (RCS) rats. The third-order neuronal response was preserved despite progressive loss of the b-wave. The results indicate that the third-order neuronal response progressively make contribution to shaping the ERG with advancing photoreceptor dege … More neration.B. Clinical application of the s-wave and PhNR1. Diabetic retinopathyOscillatory potentials (OPs) have been used as a functional indicator for early diabetic retinopathy. This indicates that dysfunction of the inner retina occurs at the early stage of diabetic retinopathy. Therefore, we compared clinical significance to detect early change of the inner retinal function in early diabetic retinopathy between the OPs and PhNR. The results demonstrated that the OPs had higher sensitivity and specificity than the PhNR, indicating that the OPs are better indicator to detect early diabetic retinopathy than the PhNR.2. Macular hole surgeryIndocyanine green (ICG) has been used to stain the inner limiting membrane during macular hole surgery. Animal studies have demonstrated that ICG injures retinal ganglion cells (RGCs) at high concentration. We compared the full-field and mf ERG between operated eyes and contralateral normal (control) eyes. Although there was no significant difference in the a-and b-waves between the operated and control eyes, the PhNR was significantly deteriorated. The s-wave of the mf ERG transiently reduced for 1 month after the surgery but returned to the pre-operated amplitude. These indicate that the PhNR and s-wave detect subclinical damage to RGCs induced by macular hole surgery.3. GlaucomaWe recorded full-field cone ERGs using white stimuli in white background (W/W) or red stimuli on blue background (R/B) The PhNR obtained by these stimulus conditions were plotted against visual sensitivity and morphometric parameters of the optic nerve head. The PhNR amplitude was significantly correlated with visual sensitivity, rim area and retinal nerve fiber layer thickness. The R/B ERG has higher correlation coefficients than the W/W ERG These indicate that the PhNR recorded by R/B is better to Less

A.基于动物实验的结果1。视神经损伤后的ERG变化通过在猫中的视神经头周围施加糖尿病来损害，我们研究了ERG随时间变化。损坏后1周，MFERG的S波消失了。另一方面，损坏后1个月明显确定光波子负反应（PHNR）。这表明MFERG在视神经损伤后比全场ERG检测到功能变化。2。与光感受器变性相关的内部视网膜的功能变化我们发现，具有遗传感受器变性的大鼠内部视网膜功能的有趣变化。我们记录了皇家外科医生学院（RCS）大鼠的ERG。第三阶神经元反应保留了B波的进行性逐渐丢失。结果表明，三阶神经元反应逐渐贡献了通过推进光感受器dege……更加neration.b的构造ERG的贡献。 S波和PHNR1的临床应用。糖尿病性视网膜病变镜（OP）已用作早期糖尿病性视网膜病的功能指标。这表明内部视网膜的功能障碍发生在糖尿病性视网膜病的早期阶段。因此，我们比较了临床意义，以检测OP和PHNR早期糖尿病性视网膜病变早期视网膜功能的早期变化。结果表明，与PHNR相比，OPS具有更高的灵敏度和特异性，这表明OPS比PHNR.2更好地指示了检测早期糖尿病性视网膜病变的早期指标。黄斑孔外科手术室内绿绿（ICG）已用于在黄斑孔手术期间染色内部限制膜。动物研究表明，ICG损伤视网膜神经节细胞（RGC）高浓度。我们比较了手术的眼睛和对比度正常（对照）眼睛之间的全场和MF ERG。尽管手术眼和对照眼睛之间的A和B波没有显着差异，但PHNR得到了显着确定。手术后，MF ERG的S波暂时降低了1个月，但返回到了经过手术的放大器。这些表明，PHNR和S波检测到黄斑孔手术引起的RGC的亚临床损伤3。 glaucomawe使用白色背景（w/w）的白色刺激或蓝色背景（r/b）在这些刺激条件下获得的PHNR在视觉神经头的视觉敏感性和形态计量参数上绘制了木核。 PHNR振幅与视觉灵敏度，边缘区域和视网膜神经纤维层厚度显着相关。 R/B ERG的相关补偿比w/w erg更高，这些指标表明，R/B记录的PHNR更好至少