Weak interactions that regulate the structure and function of the proteins

调节蛋白质结构和功能的弱相互作用

• 批准号: 17570133
• 负责人: IMAMOTO Asushi
• 金额: $ 2.24万
• 依托单位: Kyoto University (2006)Nara Institute of Science and Technology (2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570133/
• 关键词: CH; p interaction; O interaction; PYP; Photoreceptor protein; Photocycle; Hydrophobic interaction; Hydrogen bond; Structural change; CH…O相互作用; π…π相互作用; 疎水的相互作用; 光反応

Weak intramolecular interactions such as CH/O and CH/π interactions are recently proposed to be important for biomolecules, while they are several times weaker than the conventional OH/O and NH/O hydrogen bonds. In the present study, the role of these weak interactions was studied using photoactive yellow protein (PYP). The characterization of PYP mutants demonstrated that the thermal stability and photocycle rate constant of PYP are largely reduced for the mutants which lack aromatic ring at 6 position or alkyl chain at 123 position. This effect is much larger than the mutagenesis for the OH/O hydrogen bond, showing that the CH/π interaction between Phe6 and Lys123 is essential for PYP. The light-induced structural change of PYP is triggered by the breakage of the hydrophobic packing of Glu46. We showed that the CH/O interaction between carbonyl oxygen of the carboxylic acid of Glu46 and Cα of Gly29 stabilizes this hydrophobic packing. In addition, CH/π interactions between phenolic ring of the chromophore and nearby phenylalanine are essential for the stability and photocycle of PYP. These findings demonstrate that the weak interaction such as CH/O and CH/π interactions as well as the strong hydrogen bond should be taken into consideration to understand the structure and function of the proteins.

弱分子的相互作用（例如CH/π相互作用）最近被认为对生物分子比常规的OH/O氢键在本研究中更重要PYP的速率减小了在6个位置缺乏芳族环的突变体或在123位的烷基木材。 GLU46的包装。 PYP。

项目成果

Structure and photoreaction of photoactive yellow protein, a structural prototype of the PAS domain superfamily

• DOI: 10.1562/2006-02-28-ir-827
• 发表时间: 2007-01-01
• 期刊: PHOTOCHEMISTRY AND PHOTOBIOLOGY
• 影响因子: 3.3
• 作者: Imamoto, Yasushi;Kataoka, Mikio
• 通讯作者: Kataoka, Mikio

A single CH/pi weak hydrogen bond governs stability and the photocycle of the photoactive yellow protein

单个 CH/pi 弱氢键控制光活性黄色蛋白的稳定性和光循环

• 发表时间: 2006
• 期刊: J. Am. Chem. Soc. 128
• 作者: Harigai;M.;Kataoka;M.;Imamoto;Y.
• 通讯作者: Y.

A single CH/π weak hydrogen bond governs stability and the photocycle of the photoactive yellow protein

• DOI: 10.1021/ja062125v
• 发表时间: 2006-08-23
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Harigai, Miki;Kataoka, Mikio;Imamoto, Yasushi
• 通讯作者: Imamoto, Yasushi

Conformational changes of PYP monitored by diffusion coefficient : Effect of N-terminal alfa-helices

通过扩散系数监测 PYP 的构象变化：N 末端 α 螺旋的影响

• 发表时间: 2006
• 期刊: Biophys. J. 90
• 作者: Khan;J.S.;Imamoto;Y.;Harigai;M.;Kataoka;M.;Terazima;M.
• 通讯作者: M.