Direct electrochemistry of thermophilic cytochrome P450

嗜热细胞色素 P450 的直接电化学

• 批准号: 17550149
• 负责人: NAKAMURA Nobuhumi
• 金额: $ 2.3万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17550149/
• 关键词: Cytochrome P450; Electrocatalytic reaction; Immobilization of protein; Electron transfer reaction; Ionic liquid; Resonance Raman spectroscopy; Redox reaction; site-directed mutagenesis; シトクロムP450; 酸化還元電位; 直接電気化学; タンパク質固定化法; サイクリックボルタンメトリー; 表面

Cytochrome P450s catalyze the selective oxidation of inert hydrocarbon molecules with reductively activate molecular oxygen. Cytochrome P450s have a potentially useful application for biodevices such as biosensors and bioreactors. Electrochemical controlling of the cytochrome P450 catalytic reaction will cause the application to spread because of system facilitation. We investigated the direct electron transfer of P450st modified with PEO in PEO200 solvent at remarkably high temperatures.We have also studied the characterization of thermophilic cytochrome P450-didodecyldimethylammoniumbromide (DDAB) films by using UV-vis absorption, resonance Raman spectroscopy, and electrochemical methods. The observed Raman spectrum indicated near-native conformation of the heme iron in DDAB film on the surface of a glass slide, while on the surface of a plastic-formed carbon (PFC) electrode, the conformation of P450st-DDAB was very similar to that of heme-DDAB film, suggesting the release of heme from P450st in DDAB films on PFC electrodes. When NaBr was added as salt to the casting solution, the result of Raman spectrum indicated near-native conformation of P450st in DDAB film even on the PFC electrode, but no redox potential of P450st which has near native structure was observed. This study suggests the essential experimental conditions when working with heme protein-DDAB films as, in some cases, heme iron from proteins is released on the surface of the electrode.

细胞色素P450s用还原激活分子氧的惰性烃分子的选择性氧化。细胞色素P450在生物传感器和生物反应器等生物探望中具有潜在的有用应用。细胞色素P450催化反应的电化学控制将导致应用程序扩散，因为系统促进。我们研究了在高温下用PEO200溶剂修饰的P450ST的直接电子转移。我们还研究了嗜热细胞色素P450-二二二二甲基甲基氨基溴化物（DDAB）膜的表征观察到的拉曼频谱表明，载玻片表面DDAB膜中血红素铁的近构构，而在塑料形成的碳（PFC）电极的表面上，P450st-DDAB的构象与该构象非常相似血红素-DDAB膜的作品，表明在PFC电极上DDAB膜中从P450st释放血红素。当将NABR作为盐添加到铸造溶液中时，拉曼光谱的结果表明，即使在PFC电极上，DDAB膜中P450st的近乎构型，但在P450ST上也没有观察到的P450ST的氧化还原电位。这项研究表明，使用血红素蛋白-DDAB膜时，基本的实验条件是在某些情况下，蛋白质中的血红素铁在电极表面释放。

项目成果

The Electrochemical Properties of Thermophilic Cytochrome P450 CYP119A2 at Extremely High Temperature in Poly(ethylene oxide)

嗜热细胞色素 P450 CYP119A2 在聚环氧乙烷中极高温度下的电化学性能

• 发表时间: 2007
• 期刊: Electrochem.Commun. 9
• 作者: H.Matsumura;N.Nakamura;M.Yohda;H.Ohno
• 通讯作者: H.Ohno

Spectroscopic and Electrochemical Characterization of Cytochrome P450st-DDAB Films on a Plastic-Formed Carbon Electrode

塑料成型碳电极上细胞色素 P450st-DDAB 薄膜的光谱和电化学表征

• 发表时间: 2007
• 期刊: Electroanalysis 19
• 作者: H.Matsumura;K.Murata;S.Wiwatchaiwong
• 通讯作者: S.Wiwatchaiwong

A Novel Method for Direct Electrochemistry of a Thermoacidophilic Cytochrome P450

嗜热嗜酸细胞色素 P450 直接电化学的新方法

• 发表时间: 2006
• 期刊: Electrochem. Commun. 8
• 作者: S.Wiwatchaiwong;H.Matsumura;N.Nakamura;M.Yohda;H.Ohno;K.Murata;S.Wiwatchaiwong;K.Yokoyama;K.Yokoyama;松村洋寿;S.Wiwatchaiwong;H.Matsumura
• 通讯作者: H.Matsumura

Effect of Supporting Electrolytes on the Redox Potential for Thermophilic Cytochrome P450 in Poly(ethylene oxide)-With the Aim of the Utilization as a Catalyst for the Biocathode

支持电解质对聚环氧乙烷中嗜热细胞色素 P450 氧化还原电位的影响——目的是用作生物阴极催化剂

• 发表时间: 2006
• 期刊: Kobunshi Ronbunshu 63
• 作者: H.Matsumura;N.Nakamura;M.Yohda;H.Ohno
• 通讯作者: H.Ohno

Structure and Sepctroelectrochemical Property of a Ruthenium Complex Containing Phenanthroline-Quinone, and Assembly of the Complex on a Gold Electrode

菲咯啉醌钌配合物的结构和电化学性质及其在金电极上的组装

• 发表时间: 2006
• 期刊: Inorg.Chem.Acta 359
• 作者: K.Yokoyama;A.Wakabayashi;K.Noguchi;N.Nakamura;H.Ohno
• 通讯作者: H.Ohno