Production of volatile organic compounds (VOCs) by marine bacteria

海洋细菌产生挥发性有机化合物（VOC）

• 批准号: 17510015
• 负责人: HASHIMOTO Shinya
• 金额: $ 2.11万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17510015/
• 关键词: methyl iodide; marine bacterium; Alteromonas macleodii; GC-MS; Alteromonas Macleodii; Vibrio splendidus

We evaluated the potential of a marine bacterium, Alteromonas macleodii, to produce CH_3I. Bacterium was incubated at temperatures (10,15,20,25,30℃) in marine broth 2216 with or without 1 mmol/L of potassium iodide. From 15 to 30℃, the higher the culture temperature, the more rapid the proliferation rate. Proliferation rate was not affected by potassium iodide. CH_3I was produced between 15 and 30℃ with potassium iodide. Optimum temperature for CH_3I production was 25℃. A. macleodii was also incubated at 25℃ in medium spiked with potassium iodide from 0.1 to 1000μmol/L. Results showed that the greater the concentration of spiked iodide, the more CH_3I was produced. Notably, production of CH_3I strongly increased at over 10μmol/L of potassium iodide. At the concentration of iodine in surface seawater (about 0.4μmon/L), the CH_3I produced was estimated to be about 0.2μmol/L. This suggested that marine bacteria would produce about 2-20 % of CH_3I in surface seawater (the total number of bacteria in medium was 100-1000 times higher than that seen in surface seawater).

我们评估了一种海洋细菌，Alteromonas macleodii，在温度（10、15、20、25、30℃）下在含有或不含 1 mmol/L 碘化钾的海洋肉汤 2216 中培养。至30℃，培养温度越高，增殖速度越快，且不受碘化钾影响。在 15 至 30℃之间用碘化钾产生 CH_3I 的最佳温度为 25℃。在添加了 0.1 至 1000μmol/L 碘化钾的培养基中培养 A. macleodii。加标碘化物，产生的CH_3I越多。值得注意的是，当钾浓度超过10μmol/L时，CH_3I的产量急剧增加。在表层海水的碘浓度（约0.4μmon/L）下，估计产生的CH_3I约为0.2μmol/L，这表明海洋细菌在表层海水中产生约2-20%的CH_3I。培养基中的细菌总数比表层海水中的细菌总数高100-1000倍）。