The development of the vaccine for periodontal disease with using HVJ-envelope

HVJ包膜牙周病疫苗的研制

• 批准号: 16592089
• 负责人: TSURUDA Keiko
• 金额: $ 2.24万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16592089/
• 关键词: TFF peptides; HVJ-envelope; Gene transfection; Protein expression; mice; タンパク質発現; マウス; 蛋白発現; 粘液; トレフォイルファクター; TFF3; クローニング

The Trefoil factor family (TFF) peptides are localized on a mucous membrane of digestive organs of a stomach and the bowels. The TFF3 peptide is one of Trefoil factor peptides of developing antibacterial. The TFF3 specifically develops in the small intestine. Recently, the TFF3 was reported to express in submaxillary gland and parotid duct. The aim is to clarify a role of defense for inflammation of a mucous membrane of TFF3 peptide. As for this year of the last year, I examined gene transfection into mice and protein expression with a plasmid vector TFF3 connection pCAGGS, pEGF vector for the gene transfection that made it in the first year.As a result, after TFF3 gene transfection into mice by intraperitoneal injection, I dissected mice on the third day and made a freeze section of the large intestine. The fluorescence that had very weak GFP peptide was observed by fluorescent microscope. When EDTA was supplemented into HVJ-envelope transfection agent, I confirmed the clear fluorescence of GFP protein in a paneth cell neighborhood of a large intestine lamina propria. These results were concluded that protein expression by gene transfection in mice with using HVJ-envelope transfection agent was necessary to enhance the efficacy of gene transfection.in future, it is thought that a vector except a pCAGGS vector namely examination such as the vectors which connected a TFF3 gene with a pEGF vector is necessary. Furthermore, necessity of a device to raise introduction efficiency became clear.

三叶因子家族 (TFF) 肽位于胃和肠消化器官的粘膜上。 TFF3肽是开发抗菌的三叶因子肽之一。 TFF3 专门在小肠中发育。最近，据报道TFF3在颌下腺和腮腺管中表达。目的是阐明TFF3肽对粘膜炎症的防御作用。去年的今年，我用质粒载体TFF3连接pCAGGS、pEGF载体检查了基因转染到小鼠中的情况，并在第一年制作了基因转染载体pEGF。结果，TFF3基因转染到小鼠中后通过腹腔注射，第三天我解剖了小鼠并制作了大肠的冷冻切片。通过荧光显微镜观察到GFP肽的荧光非常弱。当 HVJ 包膜转染剂中添加 EDTA 时，我证实了大肠固有层潘氏细胞附近有 GFP 蛋白的清晰荧光。这些结果得出结论，使用HVJ-包膜转染剂通过基因转染在小鼠中表达蛋白质对于增强基因转染的功效是必要的。将来，人们认为除了pCAGGS载体之外的载体，即检查诸如连接有HVJ-包膜转染剂的载体的载体。带有 pEGF 载体的 TFF3 基因是必要的。此外，提高导入效率的装置的必要性也变得明确。