The roll of soluble interleukin-1 receptor type 2 for periodontal tissue

牙周组织可溶性白细胞介素1受体2型卷

基本信息

批准号：
16592082
负责人：
ISHIHARA Yuichi
金额：
$ 2.3万
依托单位：
Aichi-Gakuin university
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Head investigator and investigators have provided the results from this grant projects.(1)One hundred and eighty one gingival crevicular fluids samples were obtained from 52 periodontitis patients and interleukin-1 (IL-1)β and soluble IL-1 receptor type 2 (sIL-1Rt2) were measured by using ELISA assay kits. The concentration of IL-1β and sIL-1t2 were positive correlated with the periodontal disease severity. On the other hand, GCF IL-1b and sIL-1t2 concentrations from chronic periodontitis (CP) patients and aggressive periodontitis (AgP) patients were also examined. IL-1β was no different between CP (82.2±12.3ng/ml)and AgP(68.3±9.4ng/ml), however, AgP sIL-1Rt2(3.0±0.6ng/ml) was significantly decreased compared with CP sIL-1Rt2(1.1±0.3ng/ml). Therefore, these results suggest that AgP disease character of rapid periodontal tissue breakdown may relate to the decreased sIL-1Rt2 production. (2)A total of 102 Japanese patients and 88 periodontally healthy volunteers were recruited at the Depa … More rtment of Periodontology in Aichi-gakuin University from 2002 to 2004. Polymorphisms were identified by direct sequence method. After the single nucleotide polymorphisms (SNPs) identification, allelo- and haplotype analysis were performed. A 14.5-kb genomic region containing all exons and relevant exon-intron boundaries of IL1R1 and IL1R2 were sequenced in 24 AgPs and 24 controls. Within genomic regions containing all exons and relevant exon-intron boundaries in IL1R1 and IL1R2,38 SNPs were identified in 48 subjects. Five were located within coding elements, 2 in the 5' flanking region,22 within introns, and 9 in the 3' untranslated region. Frequency of 3 SNPs and 3 haplotypes in IL1R2 showed significant differences between patients with AgP and controls (p<0.05). (3)Gingival fibroblasts were isolated from chronic periodontitis patients. And cells were stimulated with Actinobacillus actinomycetemcomitans lipopolysaccharide, which is strongly associated with AgP. IL-1β and IL-1Rt2 mRNA gene expression were measured. However, protein production was only measured for IL-1β and not for sIL-1Rt2 in culture supernatant. Project is now undergoing for different experimental design using immunocompetent cell such as neutrophil or mononuclear cells. Less

（1）使用ELISA分析试剂盒测量了181个牙龈循环液样品，从52名牙周炎患者中获得了181个牙龈循环液样品，并使用ELISA分析试剂盒测量了181个牙龈循环液样品，并从52例牙周炎患者（IL-1）β（IL-1）β（IL-1）β（IL-1）β（IL-1）β获得了181个牙龈循环液样品。 IL-1β和SIL-1T2的浓度与牙周疾病的严重程度正相关。另一方面，还检查了还检查了慢性牙周炎（CP）患者和侵袭性牙周炎（AGP）患者的GCF IL-1B和SIL-1T2浓度。 CP（82.2±12.3ng/ml）和AGP（68.3±9.4ng/ml）之间的IL-1β并没有差异，但是，与CP SILT2（1.1±0.3NG/ml）相比，AGP SIL-1RT2（3.0±0.6ng/ml）显着降低。因此，这些结果表明，快速牙周组织分解的AGP疾病特征可能与SIL-1RT2产生的下降有关。（2）从2002年到2004年，在DEPA招募了总共102名日本患者和88名牙周健康的志愿者……在Aichi-Gakuin大学中，更多的牙周病学。通过直接序列方法鉴定了多态性。在单个核苷酸多态性（SNP）鉴定后，进行了替代性和单倍型分析。在24个AGP和24个对照中对IL1R1和IL1R2的所有外显子和相关外显子内边界的14.5-kb基因组区域进行了测序。在48名受试者中鉴定出IL1R1和IL1R2,38 SNP中包含所有外显子和相关外显子内边界的基因组区域。五个位于编码元素内，在5'侧翼区域中有2个，内含子中有22个，在3'未翻译区域中有9个。 IL1R2中3个SNP和3个单倍型的频率在患有AGP和对照的患者之间显示出显着差异（P <0.05）。（3）从慢性牙周炎患者中分离出牙龈成纤维细胞。细胞用放线肌肌动菌的脂多糖刺激，这与AGP密切相关。测量IL-1β和IL-1RT2 mRNA基因表达。然而，仅针对IL-1β而不是用于培养上清液中的SIL-1RT2的蛋白质产生。现在，使用免疫能力细胞（例如中性粒细胞或单核细胞）进行了不同的实验设计项目。较少的