Functional Analyses of new genes involved in tooth germ development

牙胚发育新基因的功能分析

• 批准号: 16591830
• 负责人: MATSUO Kou
• 金额: $ 2.24万
• 依托单位: Kyushu Dental College (2005)Kyushu University (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591830/
• 关键词: thymosin β4; phosphoglycerate kinase 1; set-α; yeast two hybrid; partner proteins; tooth germ development; thymosin β10; PGK1; GAPDH

We have performed the various kinds of analyses for elucidating the functions of genes involved in tooth germ development, which were newly found in our previous study using cDNA subtraction methods. The results are:1. thymosin P4 (TB4): The detailed expression patterns of mRNA in tooth germ development are reported in our paper (See references, Akhter, M et al.). The organ culture system using antisense-oligonucleotide against TB4 mRNA suggested the TB4 is involved in early development of tooth germ formation. The yeast two hybrid experiment revealed an actin-binding protein, a MAP kinase signal transduction protein, a lipoprotein-binding protein, a protein involved in DNA repair, a transcriptional regulator, as the partner proteins of TB4.2. Phosphoglycerate kinase 1 (Pgk1): In situ hybridization study showed that Pgk1, an enzyme in glycolytic system, play an important role in tooth germ development, in a complex with GAPDH.3. Set-a: The yeast two hybrid study suggested a transcriptional regulator, an enzyme associated with ubiquitin system, etc. may be partner proteins for set-α.4. A novel cell-adhesion factor-like protein: This hypothetical protein belongs to Immunoglobulin superfamily. The yeast two hybrid study suggested that an actin-binding protein, a lectin associated protein, an extracellular matrix associated protein, a signal transduction protein, a protein associated with cadherin, etc. as the functional partner proteins.Furthermore, we published another work associated with type II/III Run×2/Cbfal and tooth development (See references, Kobayashi et al.)

我们已经进行了各种分析，以阐明参与牙齿发育中涉及的基因的功能，这在我们先前使用cDNA减法方法的研究中是新发现的。结果是：1。胸腺素P4（TB4）：在我们的论文中报道了牙齿发育中mRNA的详细表达模式（参见参考文献，Akhter，M等人）。使用反义 - 寡核苷酸对TB4 mRNA的器官培养系统表明，TB4参与了牙齿胚芽形成的早期发展。酵母两个杂化实验揭示了一种肌动蛋白结合蛋白，一种MAP激酶信号转导蛋白，一种脂蛋白结合蛋白，一种参与DNA修复的蛋白，作为转录调节剂，作为TB4.2的伴侣蛋白。磷酸甘油酸激酶1（PGK1）：原位杂交研究表明，糖酵解系统中的一种酶在具有GAPDH.3的复合体中在牙齿发育中起重要作用。 SET-A：酵母两种杂种研究表明，转录调节剂，与泛素系统相关的酶等可能是Set-α.4的伴侣蛋白。一种新型的细胞粘附因子样蛋白：该假设蛋白属于免疫球蛋白超家族。酵母两种杂化研究表明，肌动蛋白结合蛋白，一种相关蛋白，一种相关的细胞外基质蛋白，一种信号转移蛋白，与钙粘蛋白相关的蛋白等作为功能伴侣蛋白。Furthermore.furthermore.furthermore.furthermore，我们发表了与II/III/III/III/iii Run×2/CBFal和牙齿开发相关的另一项工作（请参阅参考文献）。

项目成果

Possible functional involvement of thymosin beta 4 in developing tooth germ of mouse lower first molar

• DOI: 10.1007/s00418-005-0040-x
• 发表时间: 2005-09-01
• 期刊: HISTOCHEMISTRY AND CELL BIOLOGY
• 影响因子: 2.3
• 作者: Akhter, M;Kobayashi, I;Sakai, H
• 通讯作者: Sakai, H

Type II/III Runx2/Cbfa1 is required for tooth germ development

• DOI: 10.1016/j.bone.2005.10.026
• 发表时间: 2006-06-01
• 期刊: BONE
• 影响因子: 4.1
• 作者: Kobayashi, Ieyoshi;Kiyoshima, Tamotsu;Sakai, Hidetaka
• 通讯作者: Sakai, Hidetaka