Predicting Onset of Secondary Multiple Organ Failure by Profiling of Cytokine Gene Expression

通过细胞因子基因表达谱预测继发性多器官衰竭的发作

基本信息

批准号：
16591806
负责人：
SHIGA Hidetoshi
金额：
$ 2.11万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591806/
关键词：
SIRS gene expression cytokine IL-1 IL-6 TNF-alpha IL-10 RT-PCR IL-1β

项目摘要

Twenty ICU patients with sepsis were enrolled to the study. mRNA was isolated from the peripheral blood on admission and on day 2 in ICU. Real-time reverse transcriptase PCR was used to quantify mRNA expression of TNF-alpha, IL-1beta, IL-1 receptor antagonist, IL-6,IL-8,IL-10 and IFN-gamma. The mRNA expression levels were assessed by percent changes on day 2 compared to that on admission. Serum levels of cytokines on admission and monocyte HLA-DR expression on day 3 were also measured.Between survivors and non-survivors, there was no difference in mRNA expression levels of all mediators except for IL-10. IL-10 mRNA level on day 2 compared to that on admission was significantly up-regulated in non-survivors.When the survival rate was compared between the mRNA expression level up-regulated group and the down-regulated group, it was significantly higher in IL-10 up-regulated group.Up-regulation of IL-10 mRNA expression significantly associated with the diminished HLA-DR expression on monocytes (r=0.78,p<0.001). To the contrary, serum IL-10 level on admission did not correlate with HLA-DR expression.Up-regulated IL-10 mRNA expression in peripheral leukocytes could be a useful predictor of not only the poor outcome, but also diminishing HLA-DR expression in septic patients. The potential usefulness of IL-10 mRNA expression measurement for the management of septic patients should be further evaluated.

该研究招募了20名ICU患者。在入院时和在ICU的第2天，将mRNA从外周血中分离出来。实时逆转录酶PCR用于量化TNF-Alpha，IL-1Beta，IL-1受体拮抗剂，IL-6，IL-8，IL-8，IL-10和IFN-Gamma的mRNA表达。与入院相比，第2天的变化百分比评估了mRNA表达水平。还测量了第3天的入院和单核细胞HLA-DR表达的细胞因子水平。幸存者和非活生生之间，除IL-10外，所有介质的mRNA表达水平没有差异。与入院时相比，第2天的IL-10 mRNA水平在非残疾人中显着上调。当比较mRNA表达水平上调组和下调组之间的存活率在IL-10上调的组中明显更高。在上调的组中，IL-10 mRNA表达与IL-10 mRNA表达显着相关。IL-10 mRNA表达与hla-hla-draCy nla-draCy的表达显着相关。（r = 0.78，p <0.001）。相反，入院时血清IL-10水平与HLA-DR表达无关。受调节的IL-10 mRNA表达在外周血白细胞中不仅可以不仅可以预测不良结果，而且还可以减少化粪池患者的HLA-DR表达。应进一步评估IL-10 mRNA表达测量对化粪池患者治疗的潜在有用性。