Effects of β-citryl-L-glutamic acid and its metal ion-complex on the iron-dependent formation of free radicals

β-柠檬酰-L-谷氨酸及其金属离子络合物对铁依赖性自由基形成的影响

• 批准号: 16591776
• 负责人: MIYAKE Masaharu
• 金额: $ 1.92万
• 依托单位: Kobe Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591776/
• 关键词: β-citryl-L-glutamate,; iron; copper; competitive inhibitor,; xanthine oxidase; chelation; Feイオン依存性フリーラジカル; SOD様活性; XOD抑制活性; 鉄イオン依存性フリーラジカル

We previously isolated a novel compound containing glutamate and citrate residue from a newborn rat brain and identified it as β-citryl-L-glutamate (β-CG). This compound appears at high concentrations (300〜600μM) during the period characterized by the growth and differentiation of neurons in developing rats, and then disappears with maturation,Thin-layer chromatography results have shown that β-CG form a complex with ferrous ion and is also associated with calcium, magnesium, manganese, zinc, nickel, cobalt, and copper, where it has no affinity for ferric, potassium, or sodium ion. β-CG is able to solubilize iron more effectively from Fe(OH)_2 than Fe(OH)_3, and its Fe-solubilizing activities are inhibited by the presence of divalent metals in the following order, Co>Ca=Zn>Mp>Cu.Both β-CG and deferoxamine inhibited the iron-dependent degradation of deoxyribose in a dose-dependent manner. In contrast, EDTA activated that degradation at a low concentration, and had scant effects at a hig … More her concentration. EDTA strongly inhibited bleomycin-iron-dependent damage to DNA, while both β-CG and deferoxamine showed slight stimulatory effects at low concentrations, whereas they inhibited the damage in a dose-dependent fashion at high concentrations.Among various metal ion-complexes, only Cu-β-CG complex showed SOD activity in an assay system with xanthine-xanthine oxidase. Cu-EDTA complex also demonstrated SOD activity as did β-CG, while both Cu-and Mn-deferoxamine had strong SOD activities.The Cu-complexes of β-CG and deferoxamine showed a concentration-dependent inhibition of xanthine oxidase, while those of glutamate and citrate also demonstrated inhibitory effects, in contrast to the Cu-complex of GHK, which showed only a weak inhibitory effect, and the complexes of EDTA and bathocuproine, which had no effect. Further, free Cu^<2+> alone was also found to inhibit xanthine oxidase. Using Lineweaver-Burk plots, the type of inhibition for Cu-β-CG and free Cu^<2+> were examined, and both compounds demonstrated competitive inhibition Less

我们先前从β-CG中分离出静​​脉和柑橘酸盐残基。离子和IND与钙，镁，锰，锌，镍，钴铜有关在以下顺序中存在二价金属，β-cg和脱氧胺抑制了脱氧核糖剂量 - 偏置方式的铁的降解。她的浓度更高-EDTA复合物也像β-CG一样表现出SOD活性，而Cu-和Mn-脱氧胺都具有SOD SOD活性。β-CG和脱氧胺的Cu-复合物均表现出对Xanthine E的浓度抑制作用，而谷氨酸的浓度抑制与GHK的CU复合物相比，柠檬酸盐还表现出遗传性的抑制作用，而GHK的CU型构成了抑制黄嘌呤氧化酶的抑制作用。 >进行了检查，两者都构成了所展示的竞争性遗传往话