Study of inhibition of the metastasis in gastrointestinal carcinoma targeting for RANTES receptor

靶向RANTES受体抑制胃肠癌转移的研究

基本信息

批准号：
16591333
负责人：
FURUHATA Tomohisa
金额：
$ 2.24万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591333/
关键词：
Gastric carcinoma Colorectal carcinoma Pancreas carcinoma Invasion Stroma cell RANTES MMP HGF 乳癌 MMP-9 VEGF-A 単核球浸潤能 CCR

项目摘要

In the present study, we investigated the effect of the RANTES-mediated interaction between carcinoma cell line (gastric, colon and pancreas carcinoma) and mononuclear cell line (THP-1) in tumor progression. RANTES production in THP-1 under co-culture with carcinoma cell line was higher than in those under mono-culture. RANTES receptors were expressed in THP-1, but not in those cancer cell lines; therefore it was suggested that RANTES might affect but not cancer cells. Matrix metalloproteinase (MMP)-9 expression in THP-1 was examined. Similar to RANTES production, MMP-9 expression in THP-1 under co-culture with carcinoma cell line was higher than in those under mono-culture. Invasion assays of cancer cell lines were performed. Cancer cells cultured with THP-1 invaded into Matrigel more frequently than those without THP-1. This invasive activity was inhibited to about 50% by an anti-RANTES antibody. These results suggested that tumor cells might acquire the potential for invasion by cooperating with THP-1 mediated by RANTES and other factors might play an important role in the interplay between tumor cells and THP-1. Therefore we carried out the expression analysis of carcinoma cell lines and THP-1 by Microarray method. In this experiment, the expression of HGF, LTB and MMP-2 was up-regulated under the condition of co-culture. We will investigate whether these phenomena are observed in vivo using resection specimen in future

在本研究中，我们研究了癌细胞系（胃，结肠癌和胰腺癌）和单核细胞系（THP-1）对肿瘤介导的相互作用在肿瘤进展中的影响。与癌细胞系共培养下，THP-1中的Rantes产生高于单培养物下的癌症。 RANTES受体在THP-1中表达，但在这些癌细胞系中没有表达。因此，有人提出Rantes可能会影响但不会影响癌细胞。检查了THP-1中基质金属蛋白酶（MMP）-9的表达。与Rantes的产生类似，在与癌细胞系共培养下，THP-1中的MMP-9表达高于单培养物下的MMP-9。进行了癌细胞系的侵袭测定。用THP-1培养的癌细胞比没有THP-1的癌细胞更频繁地入侵Matrigel。这种侵入性活性被抗驱动抗体抑制至约50％。这些结果表明，肿瘤细胞可能通过与RANTES介导的THP-1合作和其他因素合作而获得侵袭的潜力，可能在肿瘤细胞与THP-1之间的相互作用中起重要作用。因此，我们通过微阵列方法对癌细胞系和THP-1进行了表达分析。在此实验中，HGF，LTB和MMP-2的表达在共培养的条件下被上调。我们将在将来使用切除样本在体内研究这些现象是否在体内观察到这些现象

项目成果

期刊论文数量（14）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Intraductal papillary muncinous neoplasms of the pancreas an analysis of protein expression and clinical features

胰腺导管内乳头状黏液性肿瘤蛋白表达及临床特征分析

DOI：
发表时间：
2006
期刊：
J Hepatobiliary Pancreat Surg. 13(4)
影响因子：
0
作者：
Nishikawa N;Kimura Y;Okita K;Zembutsu H;Furuhata T;Katsuramaki T;Kimura S;Asanuma H;Hirata K.;N.Nishikawa
通讯作者：
N.Nishikawa

The interplay between gastric cancer cell lines and PBMCs mediated by the CC chemokine RANTES plays an important role in tumor progression.

由 CC 趋化因子 RANTES 介导的胃癌细胞系和 PBMC 之间的相互作用在肿瘤进展中发挥着重要作用。