Super-high Efficient Foreign Protein Expression System in Plants

植物超高效外源蛋白表达系统

基本信息

批准号：
16580282
负责人：
MORI Masashi
金额：
$ 2.3万
依托单位：
ISHIKAWA PREFECTURAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

The aim of this study was to establish super-high efficient foreign protein expression system in plants by controlled expression of a plant virus based vector and silencing suppressors, which prevents specific degradation of viral vector RNA in a later stage of the infection. Genes of three silencing suppressors, cucumber mosaic virus 2b, tomato bushy stunt virus p19 and tomato spotted wilt virus. NSs, which suppressed gene silencing by possibly different mechanisms, were introduced to estradiol-and dexamethasone-inducible expression vectors, and transformed into Nicotiana benthamiana plants. Transgenic plants that inducibly expressed one of these suppressors were successfully produced. However, the expression of the suppressors in the cross-progenies that had two or three different suppressors were often significantly suppressed, suggesting a synergistic adverse effect of these suppressors. Next, transgenic plants that inducibly expressed one of the silencing suppressors and a replicable brome mosaic virus (BMV)-based vector by treatment of dexamethasone were successfully produced by crossing of the transgenic plants. Significantly, in the transgenic plants that inducibly expressed NSs and the BMV vector, the decreasing of the viral RNA and the foreign protein encoded in the vector, which was usually observed in a later stage of the infection, was suppressed. Various useful proteins may be produced in transgenic plants that inducibly express NSs and a BMV vector.

这项研究的目的是通过控制基于植物病毒的载体和沉默抑制子来建立植物中超高效的外国蛋白质表达系统，从而防止感染后期病毒载体RNA的特定降解。三个沉默抑制剂，黄瓜镶嵌病毒2B，番茄浓密特技病毒P19和番茄发现枯萎病毒的基因。 NSS通过可能不同的机制抑制基因沉默，引入了雌二醇和地塞米松诱导的表达载体，并转化为烟草本植物植物。成功产生了这些抑制剂之一的转基因植物。然而，在具有两个或三个不同抑制因子的跨生产物中抑制子的表达通常受到显着抑制，这表明这些抑制剂的协同不利影响。接下来，通过治疗地塞米松的处理，通过诱导表达的一种沉默抑制剂和可复制的brome马赛克病毒（BMV）载体的转基因植物成功地通过转基因植物的交叉成功产生。值得注意的是，在诱导表达NS和BMV载体的转基因植物中，病毒RNA的降低和载体中编码的异物蛋白质通常在感染的后期被抑制。可以在诱导表达NS和BMV载体的转基因植物中产生各种有用的蛋白质。

项目成果

期刊论文数量（28）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Inducible virus-mediated expression of a foreign protein in suspension-cultured plant cells

DOI：
10.1007/s00705-005-0705-8
发表时间：
2006-06-01
期刊：
ARCHIVES OF VIROLOGY
影响因子：
2.7
作者：
Dohi, K.;Nishikiori, M.;Mori, M.
通讯作者：
Mori, M.

Expression of an enhancin gene from Trichoplusia ni granulosis virus confers the resistance to lepidopterous insect pests to rice

粉纹夜蛾颗粒病毒增强素基因的表达赋予水稻对鳞翅目害虫的抗性

DOI：
发表时间：
2006
期刊：
Plant Biotechnology 23
影响因子：
0
作者：
Mori;M.;Kitamura;H.;Kondo;A.;Dohi;K.;Mori;M.;Kaido;M.;Mise;K.;Shimojyo;E.;Hashimoto,Y.
通讯作者：
Hashimoto,Y.

Genetic differences in nitrate uptake in two clones of the common reed, Phragmites australis