Development of environmental low-load-type analytical system of marine lipids

环境低负荷型海洋脂质分析系统的研制

基本信息

批准号：
16580164
负责人：
ITABASHI Yutaka
金额：
$ 2.37万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

The aim of this study was to develop an environmental low-load-type analytical system of marine lipids using high-performance liquid chromatography (HPLC), which reduces a consumption of organic solvents as much as possible. For this purpose, HPLC in conjunction with electrospray ionization mass spectrometry (ESI-MS) and HPLC with fluorescent detection were done using conventional ODS columns (250 x 4.6mm x i.d.). Chiral-phase HPLC and reversed-phase HPLC with UV detections were also applied using slurry-packed fused-silica capillaries (40cm x 0.1-0.3mm i.d.) with a various kind of chiral stationary phases and ODS, respectively. The chiral-phase HPLC analysis was optimized by using a ternary mobile phase, n-hexane-1,2-dichloroethane-ethanol. Using these techniques, we obtained successful separations of the molecular species of the glycerolipids (mono-, di-, triacylglycerols, alkylglycerols, phosphatidylcholines, phosphatidyl-ethanolamines, phosphatidylglycerols, phosphatidylinositols) including enantiomers and diastereomers. The glycerolipids were chromatographed as intact forms, 3,5-dinitrophenylurethanes and 1-anthrylurethanes. In the present study, we also achieved the separation and identification of the reverse isomers of glycerolipids, such as 1,2-diacylglycerols of 20:5-16:0 and 22:6-16:0, using reversed-phase HPLC/ESI-MS. The present study provides environmental low-load-type analytical methods of marine lipids, which can reduce a consumption of organic solvents compared to conventional HPLC methods.

这项研究的目的是使用高性能液相色谱法（HPLC）开发海洋脂质的环境低负载型分析系统，从而尽可能减少有机溶剂的消耗。为此，使用常规ODS柱（250 x 4.6mm x I.D.）进行了与电喷雾电离质谱（ESI-MS）和HPLC一起进行的HPLC。还使用泥浆包装的融合融合式毛细管（40厘米x 0.1-0.3mm i.d.）使用手性阶段的HPLC和带有紫外线检测的反向阶段HPLC，分别具有各种手性的固定相和ODS。通过使用三元流动相N-己烷-1,2-二氯乙烷 - 乙醇来优化手性HPLC分析。使用这些技术，我们获得了甘油脂脂的分子种类的成功分离（单，二 - ，三酰基甘油，烷基甘油，磷脂酰胆碱，磷脂酰乙醇胺，磷脂酰乙醇胺，磷脂酰甘油胺，磷脂酰甘油胺，磷脂酰二酰氨基糖苷）以及enterantiylyalsantialsialsials。将甘油脂质化为完整形式，3,5-二硝基苯基甲烷和1-抗甲乙烷。在本研究中，我们还实现了使用反向阶段HPLC/ESI-MS的20：5-16：0和22：6-16：0的甘油脂脂反向异构体的分离和鉴定。本研究提供了海洋脂质的环境低负载型分析方法，与常规的HPLC方法相比，可以减少有机溶剂的消耗。