Mobility and application for molecular breeding of transposon-like gene Revolver aimed genetic plant development

转座子样基因分子育种的移动性和应用 Revolver 旨在遗传植物开发

基本信息

批准号：
16580004
负责人：
TOMITA Motonori
金额：
$ 2.43万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Revolver is a new class of transposon-like gene (US-2005-0091710) discovered in the Triticeae genome. Revolver encompassing 3041 bp has 20 bp of terminal inverted repeated sequence on the both ends, which features a class II transposable element. Revolver is transcriptionally active and its cDNA clone (726 bp) contains a single ORF encoding 118 amino acid residues of protein, which shows similarity with transcriptional regulators with DNA binding ability. The entire structure of Revolver dose not share identity with both the class I and class II transposable elements ever found. Southern blot analysis on the Triticeae plants shows that Revolver is abundant in the genomes of some wheat relatives, Secale (RR), Dasypyrum (VV), as well as wheat ancestor species, Triticum monococcum (AA), T.turgidum (AABB) and T.tauschii (DD). However, Revolver is rare in the genome of bread wheat T.aestivum (AABBDD). These facts indicate that Revolver has been existed since the diploid progenitor of wheat, then it has been disappearing in bread wheat through the evolution. Quantity of Revolver in wild emmer wheat Triticum dicoccoides, which is the tetraploid progenitor of bread wheat, was examined in 17 populations (209 genotypes) representing a wide range of ecological conditions of soil, temperature and water availability in Israel. Noticeable quantitative differences in copy numbers of Revolver was observed among the populations. T.dicoccoides in the population of Yehudiyya, Ammaid, Tabigha and Mt.Gerizim, where humid and temperate, had the highest number of copies (20,000) per haploid genome and that in the population of Kokhav-Hashahar, J'aba, Amirim, Beito-Oren, Bat-Shelomo, where seasonal hot and dry wind occurs over 85 days per year, had the lowest number (50 to 100). This extensive quantitative diversity among the locations suggests mobilization of Revolver by ecological stresses.

左轮手枪是一种在Triticeae基因组中发现的新型转座子样基因（US-2005-0091710）。包含3041 bp的左轮手枪在两端具有20 bp的端子倒重复序列，该序列具有II类可转座元件。左轮手枪具有转录活性，其cDNA克隆（726 bp）包含一个编码118个蛋白质的氨基酸残基的单个ORF，该ORF与具有DNA结合能力的转录调节剂相似。左右剂量的整个结构与I类和II类可转座元素都没有共享身份。对小麦植物的Southern印迹分析表明，在某些小麦亲戚的基因组中，左右左轮杆菌很丰富，Secale（RR），Dasypyrum（VV）以及小麦祖先物种，Triticum Monococcum（AA），T.Turgidum（aabb）（AABB）和T.Tauschii（t.tauschii（ddddddddddd））。然而，在面包小麦T. t.aabbdd的基因组中，左轮手枪很少见。这些事实表明，自小麦的二倍体祖细胞以来，左轮手枪已经存在，然后它通过进化而消失在面包小麦中。在17种人群（209个基因型）中检查了野生小麦小麦双cco剂中的左轮左右左轮杆菌，这是面包小麦的四倍体祖细胞，代表了以色列的土壤，温度和水的供应量。在人群中观察到左轮手枪拷贝数的明显定量差异。 T.dicoccoides in the population of Yehudiyya, Ammaid, Tabigha and Mt.Gerizim, where humid and temperate, had the highest number of copies (20,000) per haploid genome and that in the population of Kokhav-Hashahar, J'aba, Amirim, Beito-Oren, Bat-Shelomo, where seasonal hot and dry wind occurs over 85 days per year, had the lowest number (50至100）。这些位置之间的广泛定量多样性表明，生态压力动员左轮手枪。