Mobility and application for molecular breeding of transposon-like gene Revolver aimed genetic plant development

转座子样基因分子育种的移动性和应用 Revolver 旨在遗传植物开发

基本信息

批准号：
16580004
负责人：
TOMITA Motonori
金额：
$ 2.43万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Revolver is a new class of transposon-like gene (US-2005-0091710) discovered in the Triticeae genome. Revolver encompassing 3041 bp has 20 bp of terminal inverted repeated sequence on the both ends, which features a class II transposable element. Revolver is transcriptionally active and its cDNA clone (726 bp) contains a single ORF encoding 118 amino acid residues of protein, which shows similarity with transcriptional regulators with DNA binding ability. The entire structure of Revolver dose not share identity with both the class I and class II transposable elements ever found. Southern blot analysis on the Triticeae plants shows that Revolver is abundant in the genomes of some wheat relatives, Secale (RR), Dasypyrum (VV), as well as wheat ancestor species, Triticum monococcum (AA), T.turgidum (AABB) and T.tauschii (DD). However, Revolver is rare in the genome of bread wheat T.aestivum (AABBDD). These facts indicate that Revolver has been existed since the diploid progenitor of wheat, then it has been disappearing in bread wheat through the evolution. Quantity of Revolver in wild emmer wheat Triticum dicoccoides, which is the tetraploid progenitor of bread wheat, was examined in 17 populations (209 genotypes) representing a wide range of ecological conditions of soil, temperature and water availability in Israel. Noticeable quantitative differences in copy numbers of Revolver was observed among the populations. T.dicoccoides in the population of Yehudiyya, Ammaid, Tabigha and Mt.Gerizim, where humid and temperate, had the highest number of copies (20,000) per haploid genome and that in the population of Kokhav-Hashahar, J'aba, Amirim, Beito-Oren, Bat-Shelomo, where seasonal hot and dry wind occurs over 85 days per year, had the lowest number (50 to 100). This extensive quantitative diversity among the locations suggests mobilization of Revolver by ecological stresses.

Revolver 是在小麦科基因组中发现的一类新的转座子样基因 (US-2005-0091710)。 Revolver 包含 3041 bp，两端有 20 bp 的末端反向重复序列，具有 II 类转座元件。 Revolver具有转录活性，其cDNA克隆（726 bp）含有编码118个氨基酸残基的单个ORF，与具有DNA结合能力的转录调节因子相似。 Revolver 的整个结构与迄今为止发现的 I 类和 II 类转座元件并不相同。对小麦科植物的 Southern 印迹分析表明，Revolver 在一些小麦近缘种黑麦 (RR)、Dasypyrum (VV) 以及小麦祖先物种，Triticum monocaccum (AA)、T.turgidum (AABB) 和节节线虫 (DD)。然而，Revolver 在面包小麦 T.aestivum (AABBDD) 的基因组中很少见。这些事实表明，左轮手枪自从小麦的二倍体祖先以来就已经存在，然后通过进化在面包小麦中消失了。野生二粒小麦 Triticum dicoccoides（面包小麦的四倍体祖先）中的 Revolver 数量在 17 个群体（209 个基因型）中进行了检测，这些群体代表了以色列广泛的土壤、温度和可用水生态条件。在人群中观察到左轮手枪拷贝数存在显着的数量差异。在湿润和温带的 Yehudiyya、Ammaid、Tabigha 和 Mt.Gerizim 种群中，T.dicoccoides 每个单倍体基因组的拷贝数最高（20,000），而在 Kokhav-Hashahar、J'aba、Amirim、贝托-奥伦 (Beito-Oren)、巴特-谢洛莫 (Bat-Shelomo) 每年出现超过 85 天的季节性干热风，数量最少（50 至100）。地点之间这种广泛的数量多样性表明左轮手枪是受到生态压力的影响。