Comparative analysis of replication timing among human cancer cell lines using microarray

使用微阵列比较分析人类癌细胞系的复制时间

• 批准号: 16510144
• 负责人: WATANABE Yishihisa
• 金额: $ 2.62万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16510144/
• 关键词: replication timing; p53; microarray; genome-wide; genetic instability; chromosome instability; 染色体不安定性; アンプリコン; replicatino timing; S期内複製時期; がん抑制遺伝子p53; ゲノム不安定性; DNAマイクロアレイ

The completion of the human genome sequence will greatly accelerate development of a new branch of biosience and provide fundamental knowledge to biomedical research. We used DNA microarray based on the sequence information to measure replication timing of human genome. Megabase-sized zones that replicate early or late in S phase (thus early/late transition) were defined at the sequence level. Using the newly replicated DNA from cell-cycle fractionated HCT116p53(+/+) and HCT1 116p53(-/-), the replication timing was determined and compared at the genome sequence level. In conclusion, we found that the early/late-switch regions of replication timing coincided with unstable regions of the genome and that genome-wide assessment of replication timing by DNA microarray serve as an efficient strategy for identifying cancer-related genes.

人类基因组序列的完成将极大地加速生物学的新分支，并为生物医学研究提供基本知识。我们根据序列信息使用了DNA微阵列来测量人类基因组的复制时间。在序列水平上定义了S相（因此早期/晚过渡）复制的巨大大小区域。使用来自细胞周期分离的HCT116P53（+/+）和HCT1 116P53（ - / - ）的新复制的DNA，确定并在基因组序列水平上进行比较。总之，我们发现，复制正时的早期/晚开关区域与基因组不稳定区域相吻合，并且通过DNA微阵列对复制时间的全基因组评估是鉴定癌症相关基因的有效策略。

项目成果

遺伝子多型

遗传多态性

• 发表时间: 2004
• 期刊: 病理と臨床 臨時増刊 Vol.22
• 作者: 渡辺良久;椙村春彦
• 通讯作者: 椙村春彦