Mechanisms involved in enhancement of osteoclast formation and function by periodontal cells obtained from sever orthodontic root resorption.

涉及从严重正畸牙根吸收获得的牙周细胞增强破骨细胞形成和功能的机制。

基本信息

批准号：
14571970
负责人：
KASAI Kazutaka
金额：
$ 2.18万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

The mechanism of root resorption that occurs during and after orthodontic treatment has not been clarified. We investigated the production of inflammatory cytokines and osteoclast formation after stimulus with substance P(SP) and compression force(CF) that are expressed in dental pulp and periodontal ligament(PDL) during orthodontic tooth movement. Pulp cells were obtained from 5 patients with root resorption due to orthodontic treatment, and SP (10^<-4>-10^<-12> mol/L) was added and cultured. Similarly, PDL cells were exposed compression force (0-3.0 g/cm^2). The levels of prostaglandin E_2(PGE_2), interleukin(IL)-1β,IL-6, and tumor necrosis factor(TNF)-α were determined after 24 hours. Further, culture supernatants were added to cultured rat osteoclasts, and osteoclast formation was observed after formation of resorption cavities. SP and CF stimulus increased the levels of PGE_2, IL-1β, IL-6, and TNF-α though the increase was greater in the root resorption group than in the control group, in a time- and concentration- dependent manner in pulp cells and PDL cells. As for osteoclast formation, the resorptive pits were significantly increased in the root resorption group as compared to the control. Human pulp and PDL cells obtained from patients with severe root resorption produced large amounts of inflammatory cytokines following neuropeptide and mechanical stimulus. Our results suggest that SP and CF may be highly involved in the progress of inflammation in pulp and PDL tissue and the occurrence of root resorption during orthodontic treatment.

在正畸治疗期间和之后发生的根分辨率机制尚未阐明。我们研究了用物质P（SP）和压缩力（CF）在正畸牙齿运动过程中用P（SP）和压缩力（CF）刺激后的炎性细胞因子和破骨细胞形成。从5例由于正畸治疗引起的根分辨率的患者中获得纸浆细胞，并加入并培养了SP（10^<-4> -10^<-12> mol/L）。同样，PDL细胞被暴露于压缩力（0-3.0 g/cm^2）。 24小时后确定前列腺素E_2（PGE_2），白介素（IL）-1β，IL-6和肿瘤坏死因子（TNF）-α的水平。此外，将培养上清液添加到培养的大鼠破骨细胞中，并在分辨率腔形成后观察到破骨细胞的形成。 SP和CF刺激增加了PGE_2，IL-1β，IL-6和TNF-α的水平，尽管在根回收组中的增加比对照组的增加大于浆细胞和PDL细胞中的时间和浓度依赖性方式。至于破骨细胞的形成，与对照组相比，根恢复组的吸收凹坑显着增加。从患有严重根恢复组的患者获得的人浆和PDL细胞在神经肽和机械刺激后产生大量炎症细胞因子。我们的结果表明，SP和CF可能高度参与纸浆和PDL组织的感染进展以及正畸治疗期间根分辨率的发生。