Research of the bone formation promotion effect with used together PRP from self-blood and CCEF.

自体血PRP与CCEF联合使用促进骨形成作用的研究。

• 批准号: 14571855
• 负责人: OCHI Morio
• 金额: $ 1.34万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571855/
• 关键词: Platelet-rich plasma; Osteogenesis-promot ing effect; 骨形成促進; 容量結合型電場刺激法

The aim of this study was to carry out a comparative assessment of the osteogenesis-promoting effects of platelet-rich plasma (PRP) in combination with different kinds of carrier material. We created an enclosed space by fixing a cap onto the rabbit parietal bone, in order to study osteogenesis using the guided bone regeneration (GBR) method. The space was filled with PRP along with a bone-compensating carrier to induce osteogenesis, and the amount of osteoneogenesis within the cap was measured.First, we assessed the various methods for collecting PRP. We then attached a pair of polypropylene caps to the surface of the parietal bone of each rabbit. One cap was filled with one of the following carriers: Collagen, WTCP, thrombin. β-TCP complex, or sodiumalginate (control group); the other cap was filled with therespective carrier soaked with PRP (experimental group). After 8 weeks, undecalcified samples were sectioned and used for histological observations and measurements of the amount … More of osteoneogenesis. In addition, longitudinal observations of osteogenesis guided by the β-TCP PRP complex were carried out.The most suitable method for collecting PRP was found to be a double spin method (2400 rpm for 10 mm followed by 3600 rpm for 15 mm), yielding a platelet concentration factor of 13.5. With respect to any of the carriers tested, the amount of osteoneogenesis in the cap was increased in the experimental PRP(+) group compared to the control PRP(-) group. The amount of osteogenesis was greater in the B-TCP PRP complex pair than in any of the other carrier pairs. In the longitudinal study of osteogenesis using the β-TCP PRP complex, PRP was found to increase osteogenesis relatively early in the study period.Since this experimental study confirms the osteogenesis-promoting effects of the β-TCP ・ PRP complex without the combined use of thrombin, this suggests that the 13-TCP ・ PRP complex alone will promote osteogenesis when used in clinical treatments. In addition this method, which uses no animal-derived thrombin, could have additional benefits in a clinical setting, preventing infection by unknown viruses or the side effects of abnormal blood coagulation.In 4 or 8 weeks, there was much increase of bone the PRP/β -TCP complex and CCEF stimulus modelthan PRP/β -TCP complex. Precise-ization of the bone and new bone formation promotion effect of the CCEF stimulating method was accepted. Less

本研究的目的是对富血小板血浆 (PRP) 与不同种类的载体材料结合的成骨促进作用进行比较评估。为了使用引导骨再生（GBR）方法研究骨生成，将PRP与骨补偿载体一起填充以诱导骨生成，并测量帽内的骨新生量。首先，我们评估了各种方法。然后，我们将一对聚丙烯帽固定在每只兔子的顶骨表面，其中一个帽填充有以下载体之一：胶原、WTCP、凝血酶复合物或海藻酸钠。 ）；另一个盖子装满浸有 PRP 的相应载体（实验组）。8 周后，将未脱钙的样品切片并用于组织学观察和量的测量。此外，还对 β-TCP PRP 复合物引导的成骨进行了纵向观察。发现最适合收集 PRP 的方法是双旋转法（2400 rpm，10 mm，然后 3600 rpm，15 mm）。 mm)，产生13.5的血小板浓度因子，对于任何测试的载体，与对照组相比，实验PRP(+)组中帽中的骨新生量增加。 B-TCP PRP 复合物对中的 PRP(-) 组的成骨量大于任何其他载体对。在使用 β-TCP PRP 复合物进行成骨的纵向研究中，发现 PRP 相对增加了成骨作用。由于本实验研究证实了β-TCP·PRP复合物在不联合使用凝血酶的情况下具有促进成骨的作用，因此这表明单独使用13-TCP·PRP复合物会促进骨生成。此外，这种不使用动物源性凝血酶的方法在临床治疗中可以带来额外的好处，可以预防未知病毒感染或凝血异常的副作用。在 4 或 8 周内，出现了骨生成。 PRP/β-TCP复合物和CCEF刺激模型比PRP/β-TCP复合物的骨增加更多，并且CCEF刺激方法的新骨形成促进作用较少被接受。