Research of the bone formation promotion effect with used together PRP from self-blood and CCEF.

自体血PRP与CCEF联合使用促进骨形成作用的研究。

• 批准号: 14571855
• 负责人: OCHI Morio
• 金额: $ 1.34万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571855/
• 关键词: Platelet-rich plasma; Osteogenesis-promot ing effect; 骨形成促進; 容量結合型電場刺激法

The aim of this study was to carry out a comparative assessment of the osteogenesis-promoting effects of platelet-rich plasma (PRP) in combination with different kinds of carrier material. We created an enclosed space by fixing a cap onto the rabbit parietal bone, in order to study osteogenesis using the guided bone regeneration (GBR) method. The space was filled with PRP along with a bone-compensating carrier to induce osteogenesis, and the amount of osteoneogenesis within the cap was measured.First, we assessed the various methods for collecting PRP. We then attached a pair of polypropylene caps to the surface of the parietal bone of each rabbit. One cap was filled with one of the following carriers: Collagen, WTCP, thrombin. β-TCP complex, or sodiumalginate (control group); the other cap was filled with therespective carrier soaked with PRP (experimental group). After 8 weeks, undecalcified samples were sectioned and used for histological observations and measurements of the amount … More of osteoneogenesis. In addition, longitudinal observations of osteogenesis guided by the β-TCP PRP complex were carried out.The most suitable method for collecting PRP was found to be a double spin method (2400 rpm for 10 mm followed by 3600 rpm for 15 mm), yielding a platelet concentration factor of 13.5. With respect to any of the carriers tested, the amount of osteoneogenesis in the cap was increased in the experimental PRP(+) group compared to the control PRP(-) group. The amount of osteogenesis was greater in the B-TCP PRP complex pair than in any of the other carrier pairs. In the longitudinal study of osteogenesis using the β-TCP PRP complex, PRP was found to increase osteogenesis relatively early in the study period.Since this experimental study confirms the osteogenesis-promoting effects of the β-TCP ・ PRP complex without the combined use of thrombin, this suggests that the 13-TCP ・ PRP complex alone will promote osteogenesis when used in clinical treatments. In addition this method, which uses no animal-derived thrombin, could have additional benefits in a clinical setting, preventing infection by unknown viruses or the side effects of abnormal blood coagulation.In 4 or 8 weeks, there was much increase of bone the PRP/β -TCP complex and CCEF stimulus modelthan PRP/β -TCP complex. Precise-ization of the bone and new bone formation promotion effect of the CCEF stimulating method was accepted. Less

这项研究的目的是对富含血小板的血浆（PRP）与不同种类的载体材料的促成骨的促进作用进行比较评估。我们通过将帽子固定在兔顶骨上，创建了一个封闭的空间，以便使用指导骨再生（GBR）方法研究成骨。该空间充满了PRP以及骨补偿载体以诱导成骨，并且测量了帽内的骨骼生成量。首先，我们评估了收集PRP的各种方法。然后，我们将一对聚丙烯盖连接到每个兔子顶骨的表面。一个帽子装有以下载体之一：胶原蛋白，WTCP，凝血酶。 β-TCP复合物或钠辅助（对照组）；另一个帽子充满了带有PRP（实验组）的携带载体。 8周后，将未核电的样品切除，并用于组织学观察和测量量……更多的骨负肌发生。此外，通过β-TCP PRP复合物引导的成骨的纵向观察，发现收集PRP的最合适方法是一种双自旋方法（2400 rpm，10 mm，然后为3600 rpm，15 mm的3600 rpm），产生血小板浓度为13.5。关于所测试的任何载体，与对照PRP（ - ）组相比，实验性PRP（+）组中CAP中的骨性量增加。在B-TCP PRP复合物中，成骨的量大于其他任何载体对。 In the longitudinal study of osteogenesis using the β-TCP PRP complex, PRP was found to increase osteogenesis relatively early in the study period.Since this experimental study confirms the osteogenesis-promoting effects of the β-TCP ・ PRP complex without the combined use of thrombin, this suggests that the 13-TCP ・ PRP complex alone will promote osteogenesis when used in clinical treatments.此外，这种方法使用不使用动物衍生的凝血酶，在临床环境中可能具有额外的益处，可防止未知病毒感染或异常血液凝结的副作用。在4或8周，PRP/β -TCP复合物和CCEF刺激刺激模型PRP/β -TCP复合物的骨骼大大增加。接受了骨骼和新骨形成促进效应的精确化。较少的