Comparative analysis of immune cells participating in acute and chronic rejection of kidney allografts.

免疫细胞参与同种肾移植急性和慢性排斥反应的比较分析。

• 批准号: 14571520
• 负责人: OBATA Fumiya
• 金额: $ 1.92万
• 依托单位: KITASATO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571520/
• 关键词: kidney transplantation; acute rejection; chronic rejection; cytokines; Th1; Th2; IFN-γ; IL-4; IL-10; 腎臓移植; IFN-γ

T cells mediating chronic rejection (CR) of human kidney allografts were characterized by comparing them with those mediating acute rejection (AR). Two lines of analysis were performed using biopsy specimens (23 CR and 8 AR). First, the extent of infiltration of CD4^+ and CD8^+T cells into allografts was assessed from mRNA expression of CD4 and CD8. The group of CR specimens was not significantly different from the group of AR specimens in terms of the extent of CD4^+ and CD8^+T-cell infiltration, underlining the importance of the immunological contribution to the progress of CR. Second, Th1/Th2 polarization in infiltrating T cells was investigated by measuring mRNA expression of interferon gamma (IFN-γ, a Th1 cytokine) and interleukin 4 (IL-4, a Th2 cytokine). IFN-γ expression was detected in most CR specimens, and was not significantly different between the group of CR specimens and the group of AR specimens. On the other hand, IL-4 expression was detected in only two CR specimens and one AR specimen ; from its pathological features, the AR in this last case was concomitant with CR. These results suggest that most cases of CR and of AR are mediated by Th1 mechanisms, although some cases of CR show features of both Th1 and Th2.

将介导人肾合金慢性排斥（CR）的T细胞与介导急性排斥（AR）进行比较来表征。使用活检标本（23 cr和8 ar）进行了两条分析。首先，通过CD4和CD8的mRNA表达评估了CD4^+和CD8^+ T细胞浸润的程度。在CD4^+和CD8^+ T细胞浸润的程度方面，CR标本组与AR标本组没有显着差异，这强调了免疫学对CR进展的重要性。其次，通过测量干扰素γ（IFN-γ，Th1细胞因子）和白介素4（IL-4，TH2细胞因子）的mRNA表达来研究浸润T细胞中Th1/Th2极化。在大多数CR标本中检测到IFN-γ的表达，并且CR样品组和AR标本组之间没有显着差异。另一方面，仅在两个CR样品和一个AR标本中检测到IL-4表达。从其病理特征中，在最后一个病例中，AR与CR伴随着。这些结果表明，大多数CR和AR的病例是由Th1机制介导的，尽管某些CR的情况都显示出Th1和Th2的特征。

项目成果

Funayama M: "A new locus for Parkinson's disease(PARK8) maps to chromosome 12p11.2-q13.1"Ann Neurol. 51・3. 296-301 (2002)

Funayama M：“帕金森病的新基因座（PARK8）映射到染色体 12p11.2-q13.1”Ann Neurol 51・3（2002）。

Komatsu H: "Oxidative modulation of the glutathione-redox couple enhance lipopolysaccharide-induced interleukin 12 p40 production by a mouse macrophage cell line. J774A.1"Free Radical Res. (in press).

Komatsu H：“谷胱甘肽-氧化还原对的氧化调节增强了小鼠巨噬细胞系脂多糖诱导的白细胞介素 12 p40 的产生。J774A.1”自由基研究。

Komatsu H: "An optimized method for determination of intracellular glutathione in mouse macrophage cultured by fluorometric high-performance liquid chromatography."Biomedical Chromatography. 17(5). 345-350 (2003)

Komatsu H：“一种通过荧光高效液相色谱法测定培养的小鼠巨噬细胞中细胞内谷胱甘肽的优化方法。”生物医学色谱法。

Komatsu H, Hoshino A, Funayama M, Kawahara K, Obata F.: "Oxidative modulation of the glutathione-redox couple enhance lipopolysaccharide-induced interleukin 12 p40 production by a mouse macrophage cell line, J774A.1."Free Radical Res. 37(3). 293-299 (2003

Komatsu H、Hoshino A、Funayama M、Kawahara K、Obata F.：“谷胱甘肽-氧化还原对的氧化调节增强了小鼠巨噬细胞系 J774A.1 脂多糖诱导的白细胞介素 12 p40 的产生。”自由基研究。

Komatsu H: "An optimized method for determination of intracellular glutathione in mouse macrophage cultures by fluorometric high-performance liquid chromatography."Biomedical Chromatography. 17(5). 345-350 (2003)

Komatsu H：“通过荧光高效液相色谱法测定小鼠巨噬细胞培养物中细胞内谷胱甘肽的优化方法。”生物医学色谱法。