Non-structural prosthetic valve dysfunction after aortic valve replacement : Histological and Immunohistological study

主动脉瓣置换术后非结构性人工瓣膜功能障碍：组织学和免疫组织学研究

• 批准号: 14571290
• 负责人: AOYAGI Shigeaki
• 金额: $ 2.18万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571290/
• 关键词: prosthetic valve dysfunction; pannus formation; aortic valve replacement; TGF-β1; MDCT; immunohistological mechanism; 人工弁機能不全; 血栓; 術前診断; 形質変換増殖因子; 大動脈弁置換術; TGF-β1

Objectives : Non-structual prosthetic valve dysfunction(PVD)due to thrombus and/or pannus is one of the major complications after prosthetic valve replacement. In this study, we elucidated histological and immunohistological mechanism of pannus formation. In addition, we evaluated usefulness of a multidetector-row computed tomography scanner (MDCT) for detecting causes of PVD.Methdos. : Twenty-six patients with PVD(PVD group) and randomly selected 48 patients with normal prosthetic valve function (control group) were studied. The blood was analyzed for its prothrombin time-inter national normalized ratio(PT-INR) and expression of TGF-β1. Resected pannus of reoperative patients (n=11) were used for histological(HE,EVG Azan, Grocott's) and immunohistological(TGF-β1,α-SMA,CD34,vWF etc)staining. Eight patients with PVD were underwent MDCT for detecting pannus formation before reoperation.Results : Expression of PVD group was significantly (p<0.05)grater than control group. PT-INR of PVD group was significantly (p<0.05)shorter than control group. We confirmed pannus formation was neointima with expression of TGF-β1, in the immunohistological study. We observed pannus formation detecting MDCT in 7 patients with PVD and these findings were confirmed during operation.Conclusions : In adequate anticoaglant therapy is one of cause for PVD. The pannus formation after prosthetic valve replacement may be associated with a process of periannular tissue healing via the expression of TGF-β1. MDCT can be a useful diagnostic technique for anatomic and functional evaluation of PVD as a result of pannus formation.

目的：由于替换，由于血栓和pannus引起的阀门功能障碍（PVD）是主要的并发症。 PVD的原因。对48例正常的假体瓣膜功能（对照组）进行了分析。患者（n = 11）R组织学（HE，EVG Azan，Grocott's）和免疫组织学（TGF-β1，α-SMA，CD34，VWF等）染色（P <0.05） PVD组的显着（P <0.05）我们证实了Pannus的形成是Neinernima，在TGF-β1的表达中，我们观察到7例PVD患者的Pannus形成检测MDCT，并且在手术过程中确认了这些发现。结论。 PVD。通过TGF-β1的表达，假体瓣膜替代后的Pannus形成。

项目成果

Obstruction of St.Jude Medical valve in the aortic position : Histology and immunohistochemistry of pannus.

主动脉位置 St.Jude 医疗瓣膜阻塞：血管翳的组织学和免疫组织化学。

• 发表时间: 2003
• 期刊: J Thorac Cardiovasc Surg 126
• 作者: Teshima H.;Teshima H.
• 通讯作者: Teshima H.

Usefulness of a multidetector-row computed tomography scanner for detecting pannus formation

• DOI: 10.1016/s0003-4975(03)01531-5
• 发表时间: 2004-02-01
• 期刊: ANNALS OF THORACIC SURGERY
• 影响因子: 4.6
• 作者: Teshima, H;Hayashida, N;Uchida, M
• 通讯作者: Uchida, M

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