STUDY ON THE EXPRESSION OF ESTROGEN RECEPTORS IN MALIGNANT RHABDOID TUMOR

恶性横纹肌瘤中雌激素受体表达的研究

• 批准号: 14570741
• 负责人: OHTA Shigeru
• 金额: $ 1.09万
• 依托单位: SHIGA UNIVERSITY OF MEDICAL SCIENCE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570741/
• 关键词: Malignant rhabdoid tumor; estrogen; anti-estrogen; tamoxifen; apoptosis; MRT; estrogen receptor; 悪性横紋筋様腫瘍; Malignant rhabdoid tumor

Expression of estrogen receptors and cytotoxic effects of the anti-estrogens on malignant rhabdoid tumor (MRT) cell lines were examined.Materials and Methods: Six cell lines of MRT(TM87-16, STM91-01, TTC549, TTC642, TTC1240 and YAM-RTK1) were used for the examination on the expression of estrogen receptor alpha(ERα), progesterone receptor which has some correlation to estrogen, and pS2. The effect of Tamoxifen(TAM) on the cell proliferation of MRT cell lines expressing ERα was examined. TAM has E2(estrogen) as well as anti-E2 effects. So the effect of ICI, which is a pure anti-estrogen, on the cellular proliferation of MRT cell lines was also checked.Results: The expression of ER-α was detected in TTC87-16, TTC642 and TTC1240. The expression of pS2 was not seen. The proliferation of these MRT cells was arrested with the addition of TAM and apoptosis was induced. Inhibition of cellular proliferation was note induced with ICI. The suppression of cellular proliferation was note improved with the simultaneous administration of TAM and E2.Discussion: Some of MRT cell lines expressed ER-α and showed the inhibition of cellular growth with TAM. This effect was delivered from the pathway other than ER-α. There will be a possible use of TAM as a agent for tumor dormancy therapy for MRT.

检测雌激素受体的表达和抗雌激素药物对恶性横纹肌样瘤（MRT）细胞系的细胞毒作用。 材料和方法：MRT的六种细胞系（TM87-16、STM91-01、TTC549、TTC642、TTC1240和YAM-） RTK1)用于检测雌激素受体α(ERα)、孕激素受体的表达，两者有一定的相关性。检测了他莫昔芬（TAM）对表达ERα的MRT细胞系的细胞增殖的影响，TAM具有E2（雌激素）以及抗E2作用，这是一种纯的ICI。结果：在TTC87-16、TTC642和TTC87-16中检测到ER-α的表达。 TTC1240。加入TAM后，这些MRT细胞的增殖被抑制，并且ICI诱导了细胞增殖的抑制。同时施用，细胞增殖的抑制得到了改善。 TAM 和 E2。讨论：一些 MRT 细胞系表达 ER-α，并表现出 TAM 对细胞生长的抑制作用，这种作用是通过 ER-α 以外的途径传递的。作为 MRT 肿瘤休眠治疗剂。

项目成果

太田 茂, 成田 努, 他: "Rhabdoid Tumor -基礎研究の最新知見"小児外科. 34. 440-445 (2002)

Shigeru Ota、Tsutomu Narita 等人：“横纹肌样肿瘤 - 基础研究的最新发现”小儿外科。 34. 440-445 (2002)

Shinobu Yoshida, Shigeru Ohta et al.: "TRAIL/Apo2L Ligands induce apoptosis in Malignant Rhabdoid Tumor cell lines."Pediatric Research. 54巻5号. 709-717 (2003)

Shinobu Yoshida、Shigeru Ohta 等人：“TRAIL/Apo2L 配体诱导恶性横纹肌瘤细胞系凋亡。”儿科研究，第 54 卷，第 5 期。709-717（2003 年）

太田 茂, 成田 努, 他: "Rhabdoid Tumor...基礎研究の最新知見"小児外科. 34・4. 440-445 (2002)

Shigeru Ota、Tsutomu Narita 等：“横纹肌样肿瘤……基础研究的最新发现”34・4（2002 年）。

Hirofumi Kato, Shigeru Ohta, et al.: "Expression of pericyte, mesangium and muscle markers in malignant rhabdoid tumor cell lines: Differentiation induction using 5-azacytidine"Cancer Science. 94:(10). 1059-1065 (2003)

Hirofumi Kato、Shigeru Ohta 等人：“恶性横纹肌样肿瘤细胞系中周细胞、系膜和肌肉标记物的表达：使用 5-氮杂胞苷进行分化诱导”癌症科学。

Shinobu Yoshida, Shigeru Ohta et al.: "Malignant rhabdoid tumor shows incomplete neural characteristics as revealed by expression of SNARE complex"Journal of Neuroscience Research. 69. 642-652 (2002)

Shinobu Yoshida、Shigeru Ohta 等人：“通过 SNARE 复合物的表达揭示，恶性横纹肌样瘤显示出不完整的神经特征”神经科学研究杂志。