Anticardolipin antibody and childhood epiepsy

抗心磷脂抗体与儿童癫痫

• 批准号: 14570717
• 负责人: HAGINOYA Kazuhiro
• 金额: $ 2.05万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570717/
• 关键词: Eplepsy; West syndrome; anti-cardiolipin antibody; 抗カルジオリピン杭体; western blottin; 免疫細織化学

Background : Anticardiolipin antibody (aCL) is reported to be positive in patients with antiphospholipid syndrome. However, aCL has been reported in patients with various CNS disorders. We examined the frequency of aCL in patients with various kind of epilepsy in order to clarify whether aCL is involved in the pathogenesis of childhood epilepsy. Methods : Serum samples from 175 epileptic patients and 71 non-epileptic patients were obtained after informed consent. The aCL IgG was measured using an ELISA kit purchased from MBL (Nagoya, Japan). The 175 epileptic patients included 9 with idiopathic Reneralized epilepsy (IGE), 46 with symptomatic generalized epilepsy (SGE) including 21 with West syndrome, 73 with crypiogenic or symptomatic localization-related epilepsy (C/SLE), 29 with idiopathic localization-related epilepsy, and 18 with unclassified epilepsy. Results : Although the normal range of this kit is set as below 10 U/ml in adults, we tentatively set the normal range as below 15 … More U/ml. Consequently, 24% of epileptic patients and 15% of non-epileptic patients were positive for aCL. There was no significant difference between the two groups. Among epileptic patients, SGE patients were positive significantly more often than C/SLE patients (p = 0.014, Mann-Whitney U test). When epileptic patients were divided into aCL-positive (42 patients) and aCL-negative (133 patients) groups, there were, no significant differences between the two groups in terms of sex, poly-pharmacy, antinuclear antibody, antiDNA antibody, and drugs used for treatment. However, SGE and age less than one year were significantly more frequent in the aCL-positive group (p =0.014 and <0.0001, respectively). Patients whose seizure type was epileptic spasm including West syndrome were significantly more frequent in the aCL-positive group (p = 0.0001). Conclusions : We found that aCL was present in some pediatric patients with epilepsy and other non-epileplic disorders. In all of our cases, CT and MRI showed no evidence of vascular lesions. This suggests that there is a direct cross reaction between aCL and the CNS. There was a significant relationship between aCL and those who had SGE and whose seizure type, was epileptic spasm, suggesting that aCL was involved in the pathogenesis of particular type of epilepsy including West syndrome. Less

背景：抗磷脂综合征患者据报道抗多脂蛋白抗体（ACL）为阳性。但是，已有各种中枢神经系统疾病的患者报告了ACL。我们检查了各种癫痫患者的ACL频率，以阐明ACL是否参与儿童癫痫的发病机理。方法：在知情同意后，获得了175名癫痫患者和71例非癫痫患者的血清样品。使用从MBL（日本名古屋）购买的ELISA套件测量ACL IgG。 The 175 epileptic patients included 9 with idiopathic Reneralized epilepsy (IGE), 46 with symptomatic generalized epilepsy (SGE) including 21 with West syndrome, 73 with cryptogenic or symptomatic localization-related epilepsy (C/SLE), 29 with idiopathic localization-related epilepsy, and 18 with Unclassified epileptic patients.结果：尽管该套件的正常范围设置为成年人的10 U/mL，但我们暂时将正常范围设置为15…更高的U/ml。因此，24％的癫痫患者和15％的非癫痫患者为ACL阳性。两组之间没有显着差异。在癫痫患者中，SGE患者比C/SLE患者的频率高得多（P = 0.014，Mann-Whitney U检验）。当将癫痫患者分为ACL阳性（42例患者）和ACL阴性（133例）组时，两组在性别，多种药物，抗核抗体，抗体抗体，抗体抗体和用于治疗的药物方面没有显着差异。但是，在ACL阳性组中，SGE和年龄少于一年的频率要高得多（分别为p = 0.014和<0.0001）。在ACL阳性组中，癫痫发作类型为包括西综合征在内的癫痫痉挛的患者明显更频繁（P = 0.0001）。结论：我们发现一些儿科患者患有癫痫患者和其他非雌科疾病。在我们的所有情况下，CT和MRI都没有均表现出血管病变的证据。这表明ACL和CNS之间存在直接的交叉反应。 ACL和患有SGE的人之间存在显着的关系，其癫痫发作类型是癫痫痉挛，这表明ACL参与了包括西综合征在内的特定类型的癫痫发病机理。较少的

项目成果

Munakata M, Haginoya K, et al.: "The metabolic properties of band heterotopia differ from those of other cortical dysplasias : a proton magnetic resonance spectroscopy study"Epilepsia. 44. 336-371 (2003)

Munakata M、Haginoya K 等人：“带状异位的代谢特性不同于其他皮质发育不良：质子磁共振波谱研究”癫痫。

Zhao Y, Haginoya K, et al.: "Platelet-derived growth factor and its receptors are related to disease progression in human muscular dystrophy."J Pathology. 201. 149-159 (2003)

赵 Y、Haginoya K 等人：“血小板源性生长因子及其受体与人类肌营养不良症的疾病进展有关。”J 病理学。

Munakata M, Haginoya K, Soga T, Yokoyama H, Kato R, Nagasaka T, Murata T, Higano S, Takahashi Si, Iinuma K.: "The metabolic properties of band heterotopia differ from those of other cortical dysplasias : a proton magnetic resonance spectrophotometry analy

Munakata M、Haginoya K、Soga T、Yokoyama H、Kato R、Nagasaka T、Murata T、Higano S、Takahashi Si、Iinuma K.：“带状异位的代谢特性与其他皮质发育不良的代谢特性不同：质子磁共振

Munakata M, Haginoya K, et al.: "The metabolic properties of band heterotopia differ from those of other cortical dysplasias : a proton magnetic resonance spectrophotometry analsys"Epilepsia. 44. 336-371 (2003)

Munakata M、Haginoya K 等人：“带状异位的代谢特性不同于其他皮质发育不良：质子磁共振分光光度分析”癫痫。

Zhao Y, Haginoya K, sun G, Dai H, Onuma A, Iinuma K: "Platelet-derived growth factor and its receptors are related to disease progression in human muscular dystrophy"J Pathology. 201. 149-159 (2003)

赵 Y、Haginoya K、sun G、Dai H、Onuma A、Iinuma K：“血小板源性生长因子及其受体与人类肌营养不良症的疾病进展有关”J 病理学。