Studies on the development of protective immunity with the 150-kDa lectin of Entamoeba histolytica
溶组织内阿米巴 150-kDa 凝集素发展保护性免疫的研究
基本信息
- 批准号:14570223
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have recently identified a 150-kDa surface antigen of Entamoeba histolytica as an intermediate subunit (Igl) of galactose-and N-acetyl-D-galactosamine-inhibitable lectin. In the present study, full-length Igl except for the signal sequences [amino acid (aa) 14-1,088] and three fragments of Igl, the N-terminal part (aa 14-382), middle part (aa 294-753), and C-terminal part (aa 603-1,088), were prepared in Escherichia coli and the reactivity of these recombinant proteins with sera from patients with amebiasis was examined by means of enzyme-linked immunosorbent assay (ELISA). Sera from symptomatic patients with amebic liver abscess or amebic colitis, sera from asymptomatic cyst passers, sera from individuals with other protozoan infections, and sera from healthy controls were used. Sensitivity and specificity of the recombinant full-length Igl in the ELISA were 90% and 94% respectively. When three fragments were used as antigens in the ELISA, sensitivities were 56% in the N-terminus, … More 92% in the middle part, and 97% in the C-terminus. Specificities of the three antigens were 96% in the N-terminus and 99% in both the middle and C-terminus fragments. None of the sera from asymptomatic cyst passers was negative when F-Igl, M-Igl and C-Igl were used as the antigen. On the other hand, in the cases of ELISA using M-Igl and C-Igl, false-negative results were detected in only the colitis cases. These observations suggest that the antibodies which recognized the epitopes located in M-Igl and C-Jgl may function to prevent the invasion of trophozoites into host tissues and that the carboxyl terminus of Igl is an especially useful antigen for the serodiagnosis of amebiasis. When the reactivity of adherence-inhibitory monoclonal antibodies to various recombinant fragments was also examined, one of the epitope was localized in the part of aa 989-1, 088. Liver abscess formation of hamsters was significantly inhibited by the immunization with native Igl, but not with the F-Igl, suggesting that the epitope (s) on sugar chain(s) of Igi may also be important for the induction of protective immunity. Two Igl genes, coding 1, 110 and 1, 106 aa, were also cloned from Entamoeba dispar and were further analyzed. Less
我们最近鉴定了一种 150 kDa 的溶组织内阿米巴表面抗原,作为半乳糖和 N-乙酰基-D-半乳糖胺抑制凝集素的中间亚基 (Igl)。在本研究中,除了信号序列外,全长 Igl 。氨基酸 (aa) 14-1,088] 和 Igl 的三个片段,N 端部分 (aa在大肠杆菌中制备重组蛋白(氨基酸 14-382)、中间部分(氨基酸 294-753)和 C 末端部分(氨基酸 603-1,088),并通过酶检查这些重组蛋白与阿米巴病患者血清的反应性连锁免疫吸附测定(ELISA),来自有症状的阿米巴肝脓肿或阿米巴结肠炎患者的血清,来自阿米巴肝脓肿或阿米巴结肠炎的血清。当使用三个片段作为抗原时,ELISA 中重组全长 Igl 的敏感性和特异性分别为 90% 和 94%。 ELISA,N端灵敏度为56%,中间部分为92%,中间部分为97%当 F-Igl、M-Igl 和 C 端片段的特异性为 96% 时,无症状包囊传递者的血清均呈阴性。另一方面,在使用M-Igl和C-Igl的ELISA的情况下,仅检测到假阴性结果。这些观察结果表明,识别位于 M-Igl 和 C-Jgl 中的表位的抗体可能起到防止滋养体侵入宿主组织的作用,并且 Igl 的羧基末端是对于血清诊断特别有用的抗原。当还检查了粘附抑制单克隆抗体对各种重组片段的反应性时,其中一个表位位于阿米巴病的部分。 aa 989-1, 088。用天然 Igl 免疫可显着抑制仓鼠的肝脓肿形成,但用 F-Igl 则不然,这表明 Igi 糖链上的表位对于还从迪斯帕内阿米巴中克隆了编码 1, 110 和 1, 106 aa 的两个 Igl 基因,并进一步进行了诱导。分析较少。
项目成果
期刊论文数量(34)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Y.Tsutsumi et al.: "Acanthamebic meningoencephalitis associated with alcoholic liver cirrhosis"Pathology Case Reviews. 7(6). 273-277 (2002)
Y.Tsutsumi 等人:“与酒精性肝硬化相关的棘阿米巴性脑膜脑炎”病理学病例评论。
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
Tsutsumi, Y., et al.: "Acanthamebic meningoencephalitis associated with alcoholic liver cirrhosis"Pathol.Case Rev.. 7. 273-277 (2002)
Tsutsumi, Y. 等人:“与酒精性肝硬化相关的棘阿米巴性脑膜脑炎”Pathol.Case Rev.. 7. 273-277 (2002)
- DOI:
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- 影响因子:0
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- 通讯作者:
Tachibana, H., et al.: "VH3 gene usage in neutralizing human antibodies specific for the histolytica Gal/GalNAc lectin heavy subunit"Infect.Immun.. 71(8). 4313-4319 (2003)
Tachibana, H. 等人:“VH3 基因在中和对溶组织性 Gal/GalNAc 凝集素重亚基特异的人抗体中的应用”Infect.Immun.. 71(8)。
- DOI:
- 发表时间:
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- 影响因子:0
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橘 裕司: "いま日常診療で注目すべき原虫症・寄生虫症 トリパノソーマ症・リーシュマニア症"JIM. 13. 251-253 (2003)
Yuji Tachibana:“日常临床实践中需要注意的锥虫病和利什曼病、原虫和寄生虫病”JIM 13. 251-253 (2003)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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Tachibana, H., et al.: "Evaluation of recombinant fragments of Entamoeba histolytica Gal/GalNAc lectin intermediate subunit for serodiagnosis of amebiasis"J.Clin.Microbiol.. 42(3)(印刷中). (2004)
Tachibana, H., et al.:“用于阿米巴病血清诊断的溶组织内阿米巴 Gal/GalNAc 凝集素中间亚基的重组片段的评估”J.Clin.Microbiol.. 42(3)(出版中)。
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- 影响因子:0
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TACHIBANA Hiroshi其他文献
TACHIBANA Hiroshi的其他文献
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{{ truncateString('TACHIBANA Hiroshi', 18)}}的其他基金
Establishment and evaluation of a rapid diagnosis using nanotechnology for amebiasis
利用纳米技术对阿米巴病进行快速诊断的建立和评估
- 批准号:
17K08811 - 财政年份:2017
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Isolation of pathogenic Entamoeba species from humans and macaques in Asia and analysis of host-parasite coevolution
亚洲人类和猕猴致病性内阿米巴的分离及宿主-寄生虫协同进化分析
- 批准号:
16H05819 - 财政年份:2016
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of a rapid diagnostic test for amebiasis by using fluorescent nanoparticles
使用荧光纳米粒子开发阿米巴病快速诊断测试
- 批准号:
26460516 - 财政年份:2014
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Studies on geographical distribution and genomic diversity of a new pathogenic Entamoeba species in Asia
亚洲致病性内阿米巴新种的地理分布和基因组多样性研究
- 批准号:
24406013 - 财政年份:2012
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of human monoclonal antibodies to major surface antigens of parasitic protozoa for clinical applications
开发针对寄生原虫主要表面抗原的人单克隆抗体用于临床应用
- 批准号:
23590496 - 财政年份:2011
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Polymorphic analysis of surface lectins (IGL) of Entamoeba histolytica and related Entamoeba spp.
溶组织内阿米巴和相关内阿米巴属的表面凝集素(IGL)的多态性分析。
- 批准号:
20590431 - 财政年份:2008
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Environment-Behavior Study on Physical Setting and Children's Development in Children's Nursing Home
儿童疗养院物质环境与儿童发展的环境行为研究
- 批准号:
20560584 - 财政年份:2008
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Production of human monoclonal antibodies, which inhibit in vitro growth of Plasmodium falciparum
生产抑制恶性疟原虫体外生长的人单克隆抗体
- 批准号:
18590407 - 财政年份:2006
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of charge distribution on organic cations adsorbed clay surface
粘土表面吸附有机阳离子的电荷分布分析
- 批准号:
16550163 - 财政年份:2004
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on Design and Care System of Renovated Facilities for the elderly with Small group Units
小团体老年设施改造设计及照护体系研究
- 批准号:
16560547 - 财政年份:2004
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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预防肠道阿米巴病的明矾吸收亚单位疫苗
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