Basic Mechanisms of Genetic Recombination

基因重组的基本机制

• 批准号: 04262103
• 负责人: OGAWA Hideyuki
• 金额: $ 27.26万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04262103/
• 关键词: Genetic recombination; RecA protein; RecA homologues; Rad51 protein; Transposon; Shufflon; Illegitimate recombination; Recombination hot spot; プラスミドシャフロンのDNA逆位; 組み換えホットスッポット; 抗原受容体遺伝子再構成; 相同的組換え; RecA蛋白質; ジャイレース依存の組換え; 遺伝子ターゲッティング; シャフロン

Genetic recombination is a fundamental mechanism by which parental traits of an organism are distributed to offspring. It provides a means of spreading a favorable allele and of eliminating an unfavorable allele in aspecies. Recombination must therefore have playd a crucial role in evolution of the genome. Recombination is also involved in various biological functions in a cell. It participates in repair of DNA damage, a primer formation of DNA replication, production of gene product diversities, alternative gene expression, and chromosome disjunction in meiosis I.Thus the study of recombination is crucial for thge understanding of the mechanisms of biological functions. To further our knowledge of fundamental problems of genetic recombination, a research group was organized supported by this grant. Main distingushed results obtained by this reserach group are as follows.1. The homologues of the recombination gene, recA, were successfully cloned from yeasts, chicken, mouse and human.2. … More A process of resolution of recombination intermediates formed by RecA to recombinant molecules are revealed.3. A recombinaion hot spot specific to mouse female was found and identified at the nucleotide sequence level on the chromosome 17.4. Two novel methods useful for study of genetic recombination were established.(1) A simple and easy method to get various chimera genes between homologues using a recE pathway of E.coli.(2) A comprehensive method to clone a specific site that made a genetic rearrangement from higher eukaryotes was estabilshed using in-gel competitive reassociation method.5. Various kinds of recombination proteins were identified and analyzed : for instance, Dhpl (S.pombe homologue of STPbeta in S.cerevisiae), Lim15 (lily homologues of Dmcl in S.cerevisiae), and RBP-Jkappa(mouse homologue of Su(H) in D.melanogaster).Thus a great advancement was made in analysis of basic recombination mechanism, methodology, and analysis of recombination mechanism in higher eukaryotes by this project. Less

遗传重组是一种基本机制，父母的生物是可见的。减数分裂中的染色体脱节，因此，对生物学融合的结构研究至关重要。 1。从鸡肉中成功克隆了重组基因，这是通过重组分子形成的重组S的分辨率。3。在E.Coli的同源性途径（2）中获得各种嵌合体基因。例如，DHPL（stpbeta s.cerevisiae中的S.Pombe同源物），lim15（s.cerevisie中DMCL的LILY同源性）和RBP-JKAPPA（su（H）的小鼠同源物（H）大战在分析基本的记录机制，方法论和重组机制的分析中，该项目较少

项目成果

J.Watanabe,K.Nakata,H.Nashimoto,and H.Ikeda.: "Cloning and characterization of a repetitive sequence from Pneumocystis carinii." Rarasitol.Res.78. 23-27 (1992)

J.Watanabe、K.Nakata、H.Nashimoto 和 H.Ikeda.：“卡氏肺囊虫重复序列的克隆和表征。”

K.Furukawa and Y.Hotta: "_cDNA cloning of a germ cell specific lamin B_3 from mouse spermatocyte and its functional and ectopic expression in somatic culture cells" EMBO J.12. 97-106 (1993)

K.Furukawa 和 Y.Hotta：“_从小鼠精母细胞克隆生殖细胞特异性核纤层蛋白 B_3 及其在体细胞培养细胞中的功能和异位表达”EMBO J.12。

小川智子 篠原彰 小川英行: "真核生物の組換え蛋白質Rad51とRad52の機能と構造" 実験医学(羊土社)「トッピクス」. 12. 527-547 (1994)

小川智子、筱原晃、小川英幸：“真核重组蛋白 Rad51 和 Rad52 的功能和结构”实验医学（Yodosha）“专题”。 12. 527-547（1994）。

Y.Sekine,N.Eisaki & E.Ohtsubo: "Translational Control Production of Transposase and in Trasposition of Insertion Sequence 1S3." J.Mol.Biol.235. 1406-1420 (1994)

关根永崎

Arai,N.,Kawsaki,K.,Iwabuchi,M.and Shibata,T: "Phenoltreatment and a homologous pairing-assay." Nucleic Acids Res.20. 3679-3684 (1992)

Arai,N.、Kawsaki,K.、Iwabuchi,M. 和 Shibata,T：“苯酚处理和同源配对测定。”