Visualization of Molecular Mechanism of Brain Function and Neurological Disease Using High Magnetic Field MRI

使用高磁场 MRI 可视化脑功能和神经系统疾病的分子机制

• 批准号: 17591275
• 负责人: HARADA Masafumi
• 金额: $ 2.5万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591275/
• 关键词: MRS; GABA; NAA; MEGA-PRESS; SPECT; 3T MRI; Glutamate; neurotrasmitter; MR spectroscopy; MRS; GABA; NAA; MEGA-PRESS; SPECT; 3T MRI; Glutamate; neurotrasmitter; MR spectroscopy

Gamma-aminobutyric acid (GABA) in normal subjects and patients was measured using MEGA-PRESS on clinical 3T MR apparatus and the GABAnergic index was proposed. The result of GABA nergic index was compared with the data of receptor scintigraphy in the autistic patients.Ten autistic patients (2〜11 y.o., mean 5.2 y.o.) were measured by routine anatomical MRI and after obtaining informed consents from their parents, single voxel proton MRS including MEGA-PRESS was conducted. As age-matched normal controls, six children (3〜12 y.o., mean 6.6 y.o.) who show no neurological abnormality and anatomical disorder in the brain by MRI were recruited after getting informed consents. The measured locations were left frontal lobe and left basal ganglia. The in-vitro data of NAA, Glutamine, Glutamate and GABA were acquired by MEGA-PRESS and set as a basis-set for LCModel. The quantification of signals in the difference and original spectrum by MEGA-PRESS were conducted by LCModel. A new parameter for GABAnergic neurons was defined as followed: GABAnergic index=(GABA signal from a subtracted spectra divided by NAA signal from the original spectra without selective pulses).The result of GABAnergic index in the frontal lobe and basal ganglia and this index in the frontal lobe on autistic patients were lower than that on normal controls (p<0.05). However, there is no difference in the basal ganglia between autism and normal controls. An example of the receptor scintigraphy (iomazenil SPECT) showed the activity ratio of the frontal lobe/cerebellum in autism was lower than that of the normal controls. This finding on the receptor scintigraphy was almost consistent with the result of GABAnergic index, suggesting the decrease of GABAnergic activity in the frontal lobe on autistic patients.The GABAnergic index calculated from MEGA-PRESS data would be useful for the evaluation of neurochemical disorder on autistic patients.

在临床3T MR设备上使用巨型压力测量了正常受试者的γ-氨基丁酸（GABA），并提出了Gabanergic指数。将GABA NERGIC指数与加速患者的受体闪烁图的数据进行了比较。通过常规解剖学MRI和在其父母获得知情的同意后，测量了TEN的加速患者（2-11 Y.O.，平均5.2 Y.O.），包括巨型Proton MRS，包括emga-press在内。作为年龄匹配的正常对照，六名儿童（3-12岁，平均6.6 Y.O.）在获得知情同意后，通过MRI表现出没有神经系统异常和大脑的解剖学障碍。测得的位置为左额叶和左基底神经节。 NAA，谷氨酰胺，谷氨酸和GABA的体外数据是由Mega-Press获得的，并将其设置为LCMODEL的基础。 LCMODEL进行了差异和原始光谱中信号的数量。 GABAN能神经元的一个新参数定义为：Gabanergic index =（从原始光谱中除以Naa信号的GABA信号，而没有选择性脉冲）。额叶和基础神经节中GABANergic指数的结果是自动神经节的额叶和基础叶子对自动对照的formant 5均低于正常患者（PR）。但是，自闭症和正常控制之间的基本神经节没有差异。受体闪烁显像（Iomazenil Spect）的一个例子表明，自闭症中额叶/小脑的活性比低于正常对照。对受体闪烁显像的这一发现几乎与GABAN能指数的结果一致，这表明额叶对加速患者的Gabanerctiat活性降低。根据Mega-Press数据计算的GABANergic指数可用于评估认罪患者的神经化学障碍。