Roles of chlamydial lipopoly saccharide and innate immune responses in atherogenesis

衣原体脂多糖和先天免疫反应在动脉粥样硬化形成中的作用

基本信息

批准号：
17590763
负责人：
ISHII Yuko
金额：
$ 2.37万
依托单位：
Juntendo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Chlamydiaceae are small obligate intracellular parasites and classified as gram-negative bacteria. Although recent studies have suggested that chronic inflammatory reactions by Chlamydophila pneumoniae is associated with the development of atherosclerosis, little is known about the mechanisms for Chlamydiaceae -induced host cell activation.Among Chlamydiaceae-derived components, LPS is known as a potent immunomodulator, and chlamydial LPS possesses a unique lipid A structure with longer but less acyl chains. In this study, to elucidate the chlamydial LPS-induced immune responses, we evaluated the actions of chlamydial LPS on human PBMC, using Chlamydophila psittaci LPS whose structure is nearly identical to that of C. pneumoniae, and compared with those of E. coli LPS.Similar to E. coli LPS, C. psittaci LPS bound to monocytes and induced the pro-inflammatory cytokine production in an LPS-binding protein (LBP)-dependent manner. However, C. psittaci LPS was much less potent than E. coli … More LPS in both the LPS binding and cytokine production. Interestingly, the binding of C. psittaci LPS was mediated by CD14, TLR4 and CD11b, whereas the cytokine production was dependent on CD14 and TLR4 but not CD11b. Of note, ELIS A-based binding assays revealed that C. psittaci LPS directly bound to LBP and CD14; however the affinities were much less man those of E. coli LPS. Together, these observations possibly suggest that Chlamydiaceae LPS has low binding affinities for LPS-recognition molecules such as CD14 and LBP, and exhibit weak biological activities against host immune cells including monocytes, thereby contributing to the chronic (persistent) inflammatory reactions during infection. Together, these observations indicate that chlamydial LPS has the low binding affinities for LPS-recognition molecules, and exhibits weak biological activities against host immune cells such as monocytes, thereby contributing to the chronic (persistent) inflammatory reactions during chlamydial infection. Less

衣原体科是少量的义务，被分类为革兰氏阴性细菌。在这项研究中，我们使用衣原体LPS对人类PBMC的作用，使用衣原体dophila psittaci lps，其结构与肺炎梭菌AR与大肠杆菌LPS，C。psittaciLPS BOILS，C。psittaciLPS BOILS，使用C. pssittaci LPS，使用衣原体LPS对人的PBMC的作用。单核细胞并诱导依赖性的LPS结合蛋白（LBP）中的细胞因子产生，但是，C. psittaci LPS的有效性要比大肠杆菌产生的效力要少得多。通过CD14，TLR4和CD11b，而CD14和TLR4的Toduction却不是C. psittaci LPS直接与LBP和CD14结合。对于LPS识别分子作为CD14和LBP的结合亲和力低，并且对包括单核细胞在内的宿主免疫细胞表现出弱的生物学活性，从而导致慢性（持久性）炎症反应在感染过程中。认知分子的结合亲和力低，并呼出对宿主免疫细胞（如单核细胞）的生物学活性，而衣原体感染过程中的炎症反应较少

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Role of, Chlamydial lipopolysaccharide in innate immune responses of mononuclear phagocytes.

衣原体脂多糖在单核吞噬细胞先天免疫反应中的作用。

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
Tsutsumi-Ishii Y;Shimada K;Daida H;Toman R;Nagaoka I.
通讯作者：
Nagaoka I.

エンドトキシン研究8-その新たな展開-

内毒素研究8-新进展-

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
浦野貴之;尾池雄一;石井(堤) 裕子
通讯作者：
石井(堤) 裕子

Roles of Chlamyclophila LPS in the pathogenesis of atherosclerosis-Activation of mononuclear cells by chlamysial LPS-.

衣藻 LPS 在动脉粥样硬化发病机制中的作用 - 衣藻 LPS 激活单核细胞 -。

DOI：
发表时间：
2006
期刊：
Pre Symptomatic Medicine And Anti Aging 15(1)
影响因子：
0
作者：
Tsutsumi-Ishii Y;Shimada K;Daida H;Toman R;Nagaoka I.
通讯作者：
Nagaoka I.

Innate immune responses to chlamydial LPS, activities of chlamidial LPS and immune response in host defense.

对衣原体脂多糖的先天免疫反应、衣原体脂多糖的活性和宿主防御中的免疫反应。

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
Steg PG;Bhatt DL;Wilson PWF;D'Agostino R;Ohman EM;Rother J;Liau CS;Hirsch AT;Mas JL;Ikeda Y;Pencina MJ;Goto S for the REACH Registry Investigators.;Tsutsumi-Ishii Y.
通讯作者：
Tsutsumi-Ishii Y.

Analysis of immune responses of monocytes/macrophages to chlamydial lipopolysaccharide.

单核细胞/巨噬细胞对衣原体脂多糖的免疫反应分析。