Prognostic prediction in lupus nephritis using CARP which is newly identified as a podocyte marker.

使用 CARP 来预测狼疮性肾炎的预后，CARP 是新确定的足细胞标记物。

基本信息

批准号：
17590306
负责人：
MATSUURA Keiko
金额：
$ 2.24万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590306/
关键词：
CARP lupus nephritis proteinuria renal function LPS angiotensin II adriamycin ARB adrialnycin

项目摘要

Cardiac ankyrin-repeated protein (CARP) was originally identified as a protein specifically expressed in cardiomyocytes, but has recently been found to be up-regulated in some muscle diseases including muscular dystrophy and myopathy, suggesting that CARP may be induced in some pathological conditions.In this study, we immunohistochemically analyzed renal biopsy samples from patients with glomerular diseases and two individuals with normal kidney. We found that CARP was expressed in renal podocytes at a high level in crescentic glomerulonephritis (GN), diabetic nephropathy (DN), and lupus nephritis (LN), although it was not expressed in normal kidney. Interestingly, in LN, CARP expression tended to be induced in cases exhibiting nephrotic syndrome, but less so in cases without nephrotic syndrome, suggesting that CARP expression is correlated with the severity of proteinuria.Then we analyzed the expression of CARP in podocytes of Balb/c mice that had been administrated with LPS or adria … More mycin. When LPS and adriamycin were injected to mice, CARP was induced in glomerular cells that co-express synaptopodin, a marker for podocyte, whereas CARP was not induced in mice injected with PBS. Thus, it is strongly suggested that CARP is really induced in podocytes in response to podocyte-injury. Rat glomerular parietal epithelial cell (PEC) underwent morphological changes and exhibited membrane ruffling after treatment with LPS and angiotensin II. Interestingly, phalloidin staining detected accumulation of F-actin in these areas of membrane ruffling When we treated with anagiotensin receptor blocker (ARB), morphological and distributional changes were partially blocked. On the other hand, we immunohistochemically analyzed the CARP-positive cells in urine frojm patients of GN. We could detect a small number of CARP-positive cells. Although we also tried to identify a monoclonal antibody against CARP, we have not identified it yet.In conclusion, it is suggested that immunohistochemical evaluation of CARP in biopsy samples or urine from patients with LN may be useful for diagnosis of disease severity, and probably for prediction of outcome in LN-patients. Less

心脏arnkyrin重复的蛋白质（鲤鱼）最初被鉴定为在心肌细胞中特异性表达的蛋白质，在这项研究中包括营养不良和肌病的肌病病，包括在各个节目中进行了调查。使用正常的肾脏。表达倾向于在Casses中诱导，但在情况下，在情况下，表达较少，而在情况下则与蛋白尿的严重程度相关。对小鼠的肾小球细胞诱导了鲤鱼，co -Express Synaptopodin是Podocyte的标志物，而PBS则不诱导Carp诱导上皮细胞（PEC）在使用LPS和血管紧张素II治疗后发生了形态，并表现出膜状荷叶边。 block脚。使用LN可用于诊断疾病，并且可以预测LN患者的结果。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

ARPP protein is selectively expressed in renal oncocytoma, but not in renal cell carcinomas

ARPP 蛋白在肾嗜酸细胞瘤中选择性表达，但在肾细胞癌中不表达

DOI：
发表时间：
2007
期刊：
Modern Pathology 20
影响因子：
0
作者：
Shomori K.;Nagashima Y.;Kuroda M.;Takeda A.;Tsukamoto Y.;Kawamura T.;Hiramatsu T.;Matsuura K.;Hijiya N.;Yano S.;Yokoyama S.;Ito H.;Moriyama M.
通讯作者：
Moriyama M.

Extracellular signal-regulated protein kinase is activated in cervical intraepithelial neoplasms but inactivated in invasive cervical carcinoma.

细胞外信号调节蛋白激酶在宫颈上皮内肿瘤中被激活，但在浸润性宫颈癌中失活。