INVESTIGATION INTO THE ROLE OF POTASSIUM CHANNELS IN THE LOW-OXYGEN SENSING MECHANISM IN OF AIRWAY CHEMORECEPTORS

钾离子通道在气道化学感受器低氧传感机制中的作用研究

• 批准号: 15591663
• 负责人: KOBAYASHI Noriyuki
• 金额: $ 2.11万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591663/
• 关键词: oxygen sensing mechanism; pulmonary epitherial cells; carotid body; potassium channels; 頸動脈小体

We spent a lot of time to make an electrophysiological system of patch clump. But finally we could investigate and analyze TASK-1 mRNA, one of the leak potassium channels, in neuroepitherial bodies of the lung. In histology, we could also detect TASK-1 channel in the same tissue. Ikn were enhanced by halothane and inhibited by anandamide. These observations demonstrates that TASK-1 channel play an important role in airway chemoreception. It is well known that oxygen sensing mechanisms in airway and arterial chemoreceptors are thought to be the same. We have already made clear only TASK-1 channel had a crucial role, but not Kv or BK channels in the oxygen sensing mechanisms of the carotid body as an arterial chemoreceptor, in Karolinska Institute two years ago. We had made a comparison our new data between old one in Karolinska Institute. We announced a part of our results in 13^<th> World Congress of Anaesthesiologists, Paris April, 2004. We will contribute an article of this research.

我们花了很多时间来制作一个斑块丛的电生理系统。但最终我们可以研究和分析肺神经上皮体中的 TASK-1 mRNA（泄漏钾通道之一）。在组织学中，我们还可以在同一组织中检测TASK-1通道。 Ikn 被氟烷增强并被 anandamide 抑制。这些观察结果表明 TASK-1 通道在气道化学感受中发挥重要作用。众所周知，气道和动脉化学感受器中的氧传感机制被认为是相同的。两年前，我们在卡罗林斯卡研究所就已经明确，作为动脉化学感受器的颈动脉体氧传感机制中，只有 TASK-1 通道起着至关重要的作用，而 Kv 或 BK 通道则没有。我们对卡罗林斯卡学院的新数据和旧数据进行了比较。我们在 2004 年 4 月于巴黎举行的第 13 届世界麻醉医师大会上公布了我们的部分结果。我们将发表一篇有关这项研究的文章。