Suppressive effect of hepatocyte growth factor on graft-versus-host disease in nonmyeloablative preparative regimen bone marrow transplantation for pancreatic carcinoma.

肝细胞生长因子对胰腺癌非清髓性准备方案骨髓移植中移植物抗宿主病的抑制作用。

• 批准号: 15591458
• 负责人: KURODA Nobukazu
• 金额: $ 2.18万
• 依托单位: Hyogo college of medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591458/
• 关键词: pancreatic cancer; nonmyeloablative preparative regimen; GVHD; HGF; GVT effect; hemopoietic stem cell; transplantation

This study was designed to investigate the suppressive effect of HGF(hepatocyte growth factor) on GVHD(graft-versus-host disease) in nonmyeloablative preparative regimen bone marrow transplantation as the treatment of pancreatic cancer.In original plan, the effect of HGF on GVHD suppression was determined by dose reduction of immunosuppressant agents. But it was thought to unable to determine whether the reduction of immunosuppressant agents was possible by HGF because of the deficiency of T cell and B cell.So, we considered the laboratory model. We set the site of inplant of the cancer cell subcutaneous of the back of the SCID/B6 mouse. In this model, we determined the effect of HGF on GVHD and GVT effect.After plantation of pancreatic cancer cells, bone marrow and lymphocytes of spleen were transplanted. And we determined the changes in the size of implanted tumor and GVHD effects on small intestine and liver.Our data of GVT effect were scattering, and it was difficult to quantification of GVT effects. Although t is necessary to determine in more number of experimental model, it was confirmed that HGF may have possibilities to reduce the GVHD without reduction of GVT effect.

本研究旨在探讨非清髓准备方案骨髓移植治疗胰腺癌时HGF（肝细胞生长因子）对GVHD（移植物抗宿主病）的抑制作用。通过减少免疫抑制剂的剂量来确定抑制作用。但由于T细胞和B细胞的缺乏，人们认为无法确定HGF是否可能减少免疫抑制剂的作用。因此，我们考虑了实验室模型。我们将癌细胞植入SCID/B6小鼠背部皮下的部位。在该模型中，我们测定了HGF对GVHD和GVT效应的影响。胰腺癌细胞种植后，移植骨髓和脾脏淋巴细胞。并测定了植入肿瘤大小的变化以及GVHD对小肠和肝脏的影响。我们的GVT效应数据分散，很难量化GVT效应。虽然还需要更多的实验模型来确定，但已证实HGF可能具有降低GVHD而不降低GVT效应的可能性。

项目成果

Reversal of liver fibrosis.

逆转肝纤维化。

• DOI: 10.4103/1319-3767.45072
• 发表时间: 2009-01
• 期刊: Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association
• 作者: Ismail MH;Pinzani M
• 通讯作者: Pinzani M