Washout rate of Tc-99m MIBI from myocardium in rats with diabetic cardiomyopathy

糖尿病心肌病大鼠心肌Tc-99m MIBI洗出率

• 批准号: 15591308
• 负责人: ISHIHARA Keiichi
• 金额: $ 1.79万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591308/
• 关键词: myocardial damage; doxorubicin; apoptosis; mitochondrial function; diabetic cardiomyopathy; アドリアマイシ; 心筋症; 99m-TC MIBI; 洗い出し率; トロポニンT

To validate reduced Tc-99m MIBI retention in myocardium with damaged mitochondria, myocardial MIBI uptakes of rates administrated with doxorubicin were measured in a fixed interval using gamma camera, and theses serial myocardial Tc-99m MIBI uptakes were compared with serum troponin T level as one of indicators representing ongoing myocardial damage, as well as pathological findings. Twenty Wistar-Kyoto rats of 10 weeks of age were enrolled as induced heart failure and control model animals. Five out of the ten with induced heart failure were subcutaneously administerated with 2mg/kg/W doxorubicin over 7 weeks, and the other five were administrated with the same dose of doxorubicin over 9 weeks. The ten control rats were also divided two clusters (5 each) subcutaneously administrated with the same dose of saline over 7 and 9 weeks, respectively. Blood pressure, hart rate, and body weight of each rat were measured periodically. Three minute acquisition of total and myocardial activities … More were carried out 10, 60, and 120 minutes after 3 MBq MIBI injection, using two-head gamma camera (PRISM 2000) equipped with ultra-high-resolution and pinhole collimators, respectively. Myocardial to total ratios and without ratios from 10 minutes heart to total ratio were compared between doxorubicin and control groups. Following autopsy 120 minutes after Tc-99m MIBI administration, %ID/g (percentage activity of injected dose) of heart as well as serum troponin T level were measured. Histologic scoring index by Herman and Ferrans for doxorubicin cardiomyopathy was estimated with grading on a scale of 0-4 on the basis of the number of myocytes showing cyotplasmic vacuolization and myofibrillar loss. There were significant differences of blood pressure, heart rate, and body weight after 8 weeks between doxorubicin and control groups, suggesting successful preparation for heart failure models in the former group. %ID/g of myocardium and heart to total ratios in doxorubicin group were significant lower than those in control, and washout ratios in doxorubicin group showed significantly greater values compared with those in control. All in doxorubicin group and two in control showed positive in serum troponin level, representing histologic damage of myocardium. Serum troponin T showed significant negative correlation with 10, 60, and 120 minutes heart to total ratios, respectively. Positive and negative correlation of histologic score were obtained significantly with 120 minutes heat to total ratios and 120 minutes washout ratios, respectively. Tc-99m MIBI retention in mitochondria closely related with ongoing myocardial damage in doxorubicin cardiomyopathy. Moreover, correlation of Tc-99m MIBI washout with apoptosis detection accomplished with Annexin V would indicate relationship between Tc-99m MIBI washout and mitochondrial function. On the other hand, no significant differenced were obtained between rats with diabetic cardiomyopathy administrated streptoxotocin and controls in spite of significantly decreased myocardial uptakes of Tc-99m MIBI, suggesting different mechanism between diabetic and doxorubicin heart faiuler. Less

为了验证在心肌中降低的TC-99M MIBI保留且线粒体受损的降低，使用伽马相机以固定的间隔测量了用阿霉素给药的心肌MIBI吸收速率，这些连续的心肌TC-99m tc-99m的MIBI吸收与Serrum tropotake相比，是一位均与一定程度的损害，同样是一定程度上的指标。调查结果。二十周年10周的Wistar-kyoto大鼠被招募为诱导的心力衰竭和对照模型动物。在7周内，在十个患有心力衰竭的十分之五的心力衰竭中用2mg/kg/w的阿霉素给药，另外五个在9周内用相同剂量的阿霉素给药。十只对照大鼠还分别在7周和9周内用相同剂量的盐水施用两个簇（每个5个）。定期测量每只大鼠的血压，HART率和体重。三分钟的总体和心肌活动的收购……在3 MBQ MIBI注射后进行了10、60和120分钟，分别配备了配备了超高分辨率和针孔准准直剂的两头伽马摄像头（Prism 2000）。在阿霉素和对照组之间比较了心肌与总比率，从10分钟的心脏与总比率没有比率。在TC-99M MIBI给药后120分钟尸检后，测量了心脏ID/G（注入剂量的百分比）以及血清肌钙蛋白T水平的％。赫尔曼（Herman）和法拉斯（Ferrans）对阿霉素心肌病的组织学评分指数估计，根据肌细胞的数量，表现出胞质质量真空和肌原纤维损失的级数为0-4。阿霉素和对照组之间8周后，血压，心率和体重存在显着差异，这表明在前组中成功准备了心力衰竭模型。阿霉素组的心肌和心脏与心脏与总比例的百分比明显低于对照组的百分比，并且与对照组相比，阿霉素组的冲洗比明显更高。全部在阿霉素组中，两个在对照组中都显示出血清肌钙蛋白水平阳性，代表心肌的组织学损伤。血清肌钙蛋白T分别与10、60和120分钟的心与总比率显示出显着的负相关。分别以120分钟的热比和120分钟的冲洗比显着获得组织学评分的正相关和负相关。 TC-99M线粒体中的MIBI保留与阿霉素心肌病的持续心肌损伤密切相关。此外，TC-99M MIBI冲洗与用膜联蛋白V完成的凋亡检测的相关性将指示TC-99M MIBI洗涤和线粒体功能之间的关系。另一方面，尽管具有显着改善TC-99M MIBI的心肌摄取，但在具有糖尿病心肌病的大鼠之间没有显着差异，并且对照组有明显改善，这表明糖尿病患者与阿霉素心脏失败者之间的不同机制。较少的