Interaction between neuropeptides and S100 proteins in the folliculo-sebaceous unit

毛囊皮脂单位中神经肽和 S100 蛋白之间的相互作用

• 批准号: 15591170
• 负责人: MOROHASHI Masaaki
• 金额: $ 2.24万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591170/
• 关键词: S100 proteins; Folliculo-sebaceous unit; Hair cycle; Restriction endonuclease map; Neuropeptides; Substance P; Neutral endopeptidase; Pilomatrixoma; 神経ペプチド

We examined arrangement of S100 gene, manifestation drifting of a S100 gene in hair cycle, expression of neuropeptide substance P and the precursor ppt-A, and localization of protein levels of neutral endopeptidase in the folhculo-sebaceous unit in skin. In addition, we examined manifestation of S100A protein in a pilomatrixoma.1.An arrangement of a S100 gene : We did a southern blot analysis of a BAC done including a S100A3 gene to do mapping in genome of a S100A3 gene. As a result of having hybridized S100A3, S100A4 and S100A6 probe, we understood that three genes arranged it in these on the same BAC clone. Based on these results, we obtained a restriction endonudease map of S100 gene duster of a laboratory mouse tertiary chromosome.2.Manifestation drifting of a S100 gene in hair cycle : As a result of having done Northern blot analysis about mRNA level of expression in hair cycle using antisense RNA probe for S100A mRNA, manifestation of S100A3 confined it for growth period, and man … More ifestation was found in S100A4, A6 for involution/resting phase/growth period (the first hair cycle turning point).3.We reviewed it because of manifestation of S100A2, A4, A6 protein in pilomatrixoma 22 examples immunohistologically and, about a differentiation tendency of this skin tumor, compared it with manifestation in normal folliculus pili. A constitution cell of a pilomatrixoma showed manifestation of different S100A protein partially, and it became clear to still have the differentiation potency which contributed to folliculus pili build-up.4.As a result of having immunostained substance P in ICR laboratory mouse skin and neutral endopeptidase, as for substance P, the manifestation in folliculus pili/glandulae sebaceae system showed positive to a nerve fiber of circumference connective tissue without revealing it. On the other hand, as for neutral endopeptidase, positive findings were found in outer root sheath of some folliculus pili and glandulae sebaceae marginal cell. S100A3 was strongly stained with a cuticle cell and revealed weak manifestation to a hair cortex cell. Furthermore, S100A6 mainly showed positive findings in inner root sheath. By substance P/neutral endopeptidase and double stain of S100A3, A4, A6 each, the identity was found in localization of both in neither time of hair cycle. Less

我们检查了 S100 基因的排列、S100 基因在毛发周期中的表现漂移、神经肽物质 P 和前体 ppt-A 的表达以及皮肤毛囊皮脂单位中中性肽链内切酶的蛋白质水平的定位。我们检查了毛基质瘤中 S100A 蛋白的表现。1.S100 基因的排列：我们对 BAC 进行了 Southern blot 分析，包括S100A3基因在S100A3基因的基因组中进行定位作为将S100A3、S100A4和S100A6探针杂交的结果，我们了解到三个基因排列在同一BAC克隆上。基于这些结果，我们获得了限制性内切核酸酶。实验室小鼠第三染色体S100基因簇图。2.毛发周期中S100基因漂移的表现：如使用针对 S100A mRNA 的反义 RNA 探针对毛发周期中 mRNA 表达水平进行 Northern 印迹分析，结果发现 S100A3 的表现将其限制在生长期，并且在 S100A4、A6 中发现了退化/休止期的侵染/生长期（第一个毛发周期转折点）。3.由于毛基质瘤22例中S100A2、A4、A6蛋白的表现，我们对此进行了回顾关于该皮肤肿瘤的分化倾向，通过免疫组织学与正常毛囊毛囊中的表现进行比较，可知毛基质瘤的构成细胞的一部分显示出不同的S100A蛋白质的表现，可知仍然具有有助于毛囊毛囊的分化能力。 4.由于ICR实验室小鼠皮肤中存在P物质免疫染色和中性肽链内切酶的结果，对于P物质，在毛囊中的表现菌毛/腺体系统对周围的神经纤维呈强结缔组织阳性，但未显露。另一方面，中性肽链内切酶在一些毛囊菌毛和腺体S100A3的外根鞘中发现阳性结果。角质层细胞染色，毛皮层细胞表现较弱。此外，S100A6主要在根鞘内部表现出阳性结果。 P/中性肽链内切酶和S100A3、A4、A6各自的双染，在毛发周期的任何时间都没有发现两者的定位相同。

项目成果

Aberrantly differentiated cells in benign pilomatrixoma reflect the normal hair follicle : immunohistochemical analysis of CA2+-binding S100A2, S100A3 and S100A6 proteins.

良性毛基质瘤中异常分化的细胞反映了正常毛囊：CA2 结合 S100A2、S100A3 和 S100A6 蛋白的免疫组织化学分析。

• 发表时间: 2005
• 期刊: British Journal of Dermatology 152
• 作者: Kizawa K;et al.
• 通讯作者: et al.

Aberrantly differentiated cells in benign pilomatrixoma reflect the normal hair follicle : immunohistochemical analysis of CA2+-binding S100A2,S100A3 and S100A6 proteins.

良性毛基质瘤中异常分化的细胞反映了正常毛囊：CA2 结合 S100A2、S100A3 和 S100A6 蛋白的免疫组织化学分析。

• 发表时间: 2005
• 期刊: British Journal od Dermatology 152
• 作者: Kizawa K;et al.
• 通讯作者: et al.