Development of cDNA microassay diagonostic method for detecting the chimeric genes in hematological malignancies.

开发用于检测血液恶性肿瘤中嵌合基因的cDNA微量测定诊断方法。

• 批准号: 15590478
• 负责人: FUNATO Tadao
• 金额: $ 2.18万
• 依托单位: Department of Laboratory Scinece, School of Health Science, Faculty of Medicine, Kyoto University (2005)Tohoku University (2003-2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590478/
• 关键词: hematological malignancies; DNA chip; genetic diagnosis; cDNA microarray; minimal residual disease(MRD); drug resistant gene; DNAマイクロアレイ法; 多剤耐性遺伝子; キメラ遺伝子

It is necessary to be diagnosis of a hematological malignancies such as leukemia or malignant lymphoma to use a gene analysis, karyotyping analysis and surface antigen analysisas addition to conventional morphologic inspection. In acute leukemia, identification of a factor prescribing early detection by early checkup method and convalescence is still hoped for for poor risk clinically. On the other hand, progress of recent gene analysis technology is remarkable, and cDNA microarray technology as DNA chip is above all most suitable though presence of gene expression. This study foced on the cDNA microarray method and developed this method that integrated the chimera gene which specifically appeared for leukemia. As a to be aimed at developing shaped cDNA microarray as early checkup, and having examined various kinds of nucleic acid fixation technology, it be fixed the slide that treated this resin by letting you do covalent bond of a 'DNA oligomer. Furthermore, it could be made a result viewing by using color development ground substance. Therefore, cDNA microarray technology which combined this immobilized technology with the simple detection enabled what the various kinds of chimera genes which were specific for leukemia

白血病或恶性淋巴瘤等血液系统恶性肿瘤的诊断除了常规的形态学检查外，还需要使用基因分析、核型分析和表面抗原分析。对于急性白血病，由于风险较低，临床上仍希望通过早期检查方法识别出早期发现的因素并进行康复治疗。另一方面，最近的基因分析技术的进步是显着的，尽管存在基因表达，但作为DNA芯片的cDNA微阵列技术尤其是最合适的。本研究着眼于cDNA微阵列方法，开发了这种整合白血病特异性嵌合基因的方法。作为早期检查的目的，为了开发成形的cDNA微阵列，研究了各种核酸固定技术，通过让“DNA低聚物”进行共价键合，将处理过该树脂的载玻片固定化。另外，还可以使用显色底物进行结果观察。因此，将这种固定化技术与简单检测相结合的cDNA微阵列技术，使得能够对白血病特异的各种嵌合基因进行检测。

项目成果

Utility of antioncogene ribozymes and antisense oligonucleotides in reversing drug resistance.

抗原基因核酶和反义寡核苷酸在逆转耐药性中的效用。

• 发表时间: 2005
• 期刊: Methods Mol Med. 106
• 作者: Abe S;Funato T;Kameoka J;Funato T
• 通讯作者: Funato T

Increased Expression of Insulin-Like Growth Factor I is Associated with Are-C Resistance in Leukesia.

胰岛素样生长因子 I 表达的增加与白血病中的 Are-C 耐药性相关。

• 发表时间: 2006
• 期刊: Tohoku J Exp Med. 209(3)
• 作者: Abe S;Funato T;Kameoka J
• 通讯作者: Kameoka J

Detection of clone-specific immunoglobulin heavy chain genes in the bone marrow of B-cell-lineage

B 细胞谱系骨髓中克隆特异性免疫球蛋白重链基因的检测

• 发表时间: 2004
• 期刊: Tohoku J Exp Med 203(3)
• 作者: Hoshino A;Funato.T;et al.
• 通讯作者: et al.

舩渡 忠男: "造血器腫瘍における新しい遺伝子診断法の開発"臨床病理. 52・2. 162-166 (2004)

Tadao Funato：“造血系统肿瘤的新遗传诊断方法的开发”临床病理学52・2（2004）。

Stanniocalcin-1 am a novel marker to detect minimal residual dimeasa of human leukemia.

Stanniocalcin-1 是一种检测人类白血病微小残留二聚体的新型标记物。

• 发表时间: 2004
• 期刊: Tohoku J Exp Med Oct;2004(2)
• 作者: Tohmiya Y;Funato T;Kameoka J
• 通讯作者: Kameoka J