Study of antiviral and anticancer effects of polyphenols

多酚的抗病毒和抗癌作用研究

基本信息

批准号：
15590238
负责人：
NAKASHIMA Hideki
金额：
$ 2.05万
依托单位：
St.Marianna University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

1)Antiviral study(1)Anti-HTV assay : MT-4 cells were infected with HIV-I and incubated in the presence of various concentrations of test compounds. After incubation for 5 days, cell viability was quantified by MTT assay to estimate a inhibition activity of test compounds against HIV induced CPE. Test compounds were alkyl-ologosaccharides, synthetic peptides, and an extract from, an Indian medical plant, Indigofera cordifora.(2)Anti-HSV assay : HSV-1 infected Vero cells were incubated in the presence of test compounds and antiviral activity was evaluated as the inhibition activity against virus induced CPE.2)Study for anticancer activities(1)Assay for cytotoxic activity : Three human oral tumor cell lines, (HSG, HSC-2, HSC-3), and 3 human normal cells, (HGF, HPC, HPLF) were incubated in the presence of test compounds. After 24 h incubation, the relative viable cell number was detenraned by MTT assay.(2)Assay for DNA fragmentation : DNA fragmentation from cultured cells with test compoun … More ds treatment was determined to estimate the inhibitory activity against apoptosis.(3)L5179 mouse T cell lymphoma cell line was transfected with MDR1/A containing retrovirus and MDR1 expressing cells were selected. The reversed activity of test compounds were studied.(4)Caspase activation and anti-radical activity using by ESR spectroscopy were also carried out.(5)Anti-melanoma metastatic activity was assessed using an experimental pulmonary metastasis assay of B16-BL6 melanoma cells.According to the results of these experiments, extracts from several plants, such as Anastasia Red, demonstrate anticancer activity and inhibition of MDR expression. Tropolone derivatives have anticancer activity due to radical mediated redox reaction. 4F-benzoyl-TE14011,an analog of synthetic peptide T22 and CXCR4 antagonist, was encapsulating with a biodegradable polymer, poly D, L-lactic acid (PLA). Subcutaneous single administration of 4F-benzoyl-TE14011-PLA significantly reduced the number of colonies formed by pulmonary metastasis of B16-BL6 melanoma cells. Less

1）反HTV分析（1）抗HTV测定：MT-4细胞使用HIV-I并在孵育5天后孵育的情况下孵育5天。针对HIV的化合物诱导CPE。（HSG，HSC-2，HSC-3）和3个人正常细胞（HGF，HPC，HPLF）被测试化合物孵育。使用MDR1/综合的研究，使用B16-BL6黑色素瘤细胞进行了测试。证明抗癌活性和因子介导的反应而具有作用。黑色素瘤细胞。

项目成果

期刊论文数量（99）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

微生物学の歴史I、II (監訳)

微生物学史 I、II（监督翻译）

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
Fujii;N.;Oishi;S.;Hiramatsu;K.;Araki;T.;Ueda;S.;Tamamura;H.;Otaka;A.;Kusano;S.;Terakubo;S.;Nakashima;H.;Broach;J.A.;Trent;J.O.;Wang;Z.X.;Peiper;S.C.;嶋田甚五郎
通讯作者：
嶋田甚五郎

Susceptibilities of Fluoroquinolone-resistant Haemophilus para-influenzae isolates from Japanese patients with respiratory infections to five Fluoroquinolones and other antimicrobial agents.

日本呼吸道感染患者分离出的耐氟喹诺酮类副流感嗜血杆菌对五种氟喹诺酮类药物和其他抗菌药物的敏感性。

DOI：
发表时间：
2003
期刊：
Antimicrob.Agents Chemother. 47
影响因子：
0
作者：
Takemura;H.
通讯作者：
H.

Motohashi, N., Wakabayashi, H., Nakashima, H., et al.: "Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper"PHYTOTHERAPY RES.. 17. 348-352 (2003)

Motohashi, N.、Wakabayashi, H.、Nakashima, H. 等人：“蔬菜‘Anastasia Red’、各种甜椒的细胞毒性和多药耐药性逆转活性”PHYTOTHERAPY RES.. 17. 348-352

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Current status of delivery systems to improve target efficacy of oligonucleotides.

DOI：
10.2174/1381612043453009
发表时间：
2004-02
期刊：
Current pharmaceutical design
影响因子：
3.1
作者：
Y. Shoji;H. Nakashima
通讯作者：
Y. Shoji;H. Nakashima

Cytotoxic activity of Azulenequiiiones against human oral tumor cell lines.

Azulenequiiones 对人口腔肿瘤细胞系的细胞毒活性。

DOI：
发表时间：
2005
期刊：
Anticancer Res. 25
影响因子：
0
作者：
Wakabayashi;H.;Nishishiro;M.;Arikawa;S.;Hashimoto;K.;Kikuchi;H.;Nishikawa;H.;Kurihara;T.;Terakubo;S.;Shoji;Y.;Nakashima;H.;Motohashi;N.;Sakagami;H.
通讯作者：
H.