Identification and functional analyses of CO_2-sensing cells in the central respiratory chemoreceptor

中枢呼吸化学感受器CO_2感受细胞的鉴定及功能分析

基本信息

批准号：
15590190
负责人：
OKADA Yasumasa
金额：
$ 2.3万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In this research project we have studied the function and anatomy of the neural substrate for the central respiratory chemoreception and central respiratory control.In order to identify the functional role of an important neurotransmitter GABA in central respiratory control, we used GABA-synthesizing enzyme (GAD) gene-targeted mice, and found that GABA is necessary in the maintenance of stable respiratory output (Kuwana et al. 2003 ; 2004 ; 2006). We also found that respiratory depression by propofol is induced by its GABA-like inhibitory action (Kashiwagi et al. 2004 ; 2004).We applied a voltage imaging technique to an in vitro brainstem preparation, and succeeded functional mapping of chemosensitive regions. We reported that the pontine A5 region has intrinsic chemosensitivity (Ito et al. 2004).On the basis of our hypothesis that nicotinic acetylcholine receptor is importantly involved in central respiratory chemoreception, we analyzed the effects of non-depolarizing muscle relaxants on chemoresponsiveness in vitro. We first reported that non-depolarizing muscle relaxants inhibit respiratory activity in the brainstem (Sakuraba et al. 2003 ; 2005).We summarized the results of our studies, and have proposed our theories that potassium channels play a key role as the chemosensor in chemoreceptor cells (Oyamada et al. 2006) and that specific small cells surrounding fine vessels in the superficial ventral medulla are primary chemoreceptor cells (Okada et al. 2006).

在该研究项目中，我们研究了中央呼吸性化学感受和中央呼吸控制的神经底物的功能和解剖结构。为了确定重要的神经递质GABA在中央呼吸道控制中的功能作用，我们使用了GABA合成酶（GABA）的基因（GAD）基因的含量含量为GABA的鼠标均具有良好的输出，以实现GABA的水平，以实现GABA的需求，以实现GABA的需求。（Kuwana等人2003; 2004; 2006）。我们还发现，丙泊酚的呼吸道抑郁是由其GABA样抑制作用诱导的（Kashiwagi等，2004; 2004）。我们将电压成像技术应用于体外脑干的制备，并成功地对化学敏感区域进行了功能映射。我们报道说，蓬托A5区具有固有的化学敏感性（Ito等，2004）。在我们的假设的基础上，烟碱乙酰胆碱受体在中央呼吸道化学受感受中很重要，我们分析了非抑制肌肉松弛剂对化学疗法的影响。 We first reported that non-depolarizing muscle relaxants inhibit respiratory activity in the brainstem (Sakuraba et al. 2003 ; 2005).We summarized the results of our studies, and have proposed our theories that potassium channels play a key role as the chemosensor in chemoreceptor cells (Oyamada et al. 2006) and that specific small cells surrounding fine vessels in the superficial ventral medulla are primary chemoreceptor cells （Okada等，2006）。