Molecular evaluation of volatiles in plant defense

植物防御中挥发物的分子评价

• 批准号: 15580035
• 负责人: AKIMITSU Kazuya
• 金额: $ 2.11万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580035/
• 关键词: citrus; monoterpene; plant; non-mevalonic acid pathway; signal transduction; Alternaria; ラフレモン; 抵抗性; 揮発性物質; 傷処理; 菌接種

Various types of volatiles have been known to produce and release from plant cells. We have studied roles and importance of several different volatiles including monoterpenes, which are the major component of citrus volatiles. Eleven monoterpenes were tested their biological functions, such as fungicidic acitivity, anti-insect activity, and signal transduction activity. Mevalonic acid and non-mevalonic acid pathways have been Known to be involved for biosynthesis of terpenoids and C10 monoterpenes would be synthesized by GPP from non-mevalonic acid pathway in chloroplast through monoterpene pathway. There are at least seven enzymes involved in non-mevalonic acid pathway, and a citrus gene encoding one of these enzymes, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), was cloned. A localization analysis using GFP as the reporter indicated that the gene product is located in chloroplast.

已知各种类型的挥发物可以从植物细胞中产生和释放。我们研究了几种不同挥发物在内的角色和重要性，包括单二烯，这是柑橘挥发物的主要组成部分。测试了11种单二烯的生物学功能，例如杀真菌性阳性，抗iNsect活性和信号转导活性。众所周知，已知甲瓦膦酸和非杂种酸途径与萜类化合物的生物合成有关，而C10单烯烯将由GPP从叶绿体中通过单萜的叶绿体中的非杂质酸途径合成。克隆了至少有7种酶，而编码这些酶之一的柑橘基因，即2-C-甲基-D-雄性2,4-氯二章磷酸合酶（ISPF）。使用GFP作为记者的定位分析表明，基因产物位于叶绿体中。

项目成果

山崎 祐未子, 田村 啓敏, 久能 均, 山本 弘幸, 秋光 和也: "Alternaria alternataを噴霧接種したラフレモン葉からのモノテルペン放出様式"日本植物病理学会講演要旨集. 12 (2003)

Yumiko Yamazaki、Keitoshi Tamura、Hitoshi Kuno、Hiroyuki Yamamoto、Kazuya Akimitsu：“喷雾接种链格孢菌的粗糙柠檬叶中的单萜释放模式”日本植物病理学学会会议记录 12 (2003)。

Alternaria diseases of citrus - Novel pathosystems.

柑橘链格孢病 - 新的病理系统。

• 发表时间: 2003
• 期刊: Phytopathologia Mediterranea 42
• 作者: Timmer;L.W.et al.
• 通讯作者: L.W.et al.

植物病の探求

植物病害探索

• 发表时间: 2004
• 作者: 露無慎二;石原博道;藤川貴史
• 通讯作者: 藤川貴史

山崎祐未子, 久能 均, 山本弘幸, 秋光和也: "カンキツ病害抵抗性誘導における揮発性モノテルペンの役割について"日本植物病理学会講演要旨集. 164 (2004)

Yumiko Yamazaki、Hitoshi Kuno、Hiroyuki Yamamoto、Kazuya Akimitsu：“挥发性单萜在诱导柑橘抗病性中的作用”日本植物病理学学会会议记录 164 (2004)。

Biology of Molecular Plant-Microbe Interactions, Volume 4

分子植物-微生物相互作用生物学，第 4 卷

• 发表时间: 2004
• 作者: Ohtani;K.;Masunaka;A.;Yamasaki;Y.;Yamamoto;H.;Akimitsu;K.
• 通讯作者: K.