Bacterial trapping near topographic surfaces under shear flow

剪切流下地形表面附近的细菌截留

• 批准号: 462445093
• 负责人: Professor Dr. Holger Stark
• 金额: --
• 依托单位: Institut für Theoretische Physik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/462445093?language=en
• 关键词: Bacterial; trapping; near; topographic; surfaces

Motile bacteria, such as Escherichia coli (E. coli), colonize surfaces, where they form hazardous biofilms and cause biofouling. When reaching the surface, they first need to be trapped by physical mechanisms, which then promotes their irreversible attachment to the surface through chemical bonding. Despite much insight, near-surface trapping of E. coli is still a poorly understood complex process, which is determined by E. coli’s run-and-tumble motility and physical conditions, such as shear flow and the surface topography. However, controlling near-surface trapping is essential for medical and biotechnological applications, for example, for preventing biofilms and biofouling but also for using bacteria as drug carriers to target disease sites such as malignant tumors. Therefore, the Indian and German groups join forces and use their complementary expertises to carry out a comprehensive simulation analysis in order to explore how physical conditions can be used to control the trapping of E. coli in shear flow near surfaces with different topography.We will perform a step-by-step analysis of the complex problem and heavily rely on a realistic model E. coli developed earlier by the Indian project leader. It will be used for a thorough preparatory analysis of the run-and-tumble motion in the bulk. In parallel, the German group will implement the model E. coli in a code based on the method of Multi-Particle Collision Dynamics, which will enable us to simulate fluid flows around the bacterium near surfaces with varying topography. Sharing the code between both groups, we will thoroughly analyze how a motile E. coli becomes trapped near a surface either in a quiescent fluid or under shear flow and thereby clarify contradicting observations. Our foci will be on the role of flagellar dynamics during runs and tumbles including polymorphism, which has so far been not resolved in any of the reported experimental studies on surface trapping, and also on the role of rheotaxis and Jeffery orbits for the bacterial dynamics. Finally, we will model surfaces with non-planar topography and investigate situations related to the control of biofouling and targeted drug deposition.

流动细菌，例如大肠杆菌（大肠杆菌），整体表面，整个形式的生物膜并引起生物污染物，然后通过化学结合促进其对表面的不可逆转。尽管如此，这是由E.跑步运动和物理状况（例如剪切流和表面形状）确定的，控制近表面陷阱对于医学和生物技术应用至关重要，但也用于使用细菌诸如恶性肿瘤之类的靶向疾病的载体。通过对复杂问题进行分步分析，并严重依赖于印度项目领导者开发的大肠杆菌。组将基于熔体粒子碰撞动力学的代码中的大肠杆菌，这将使我们能够模拟细菌neal的液体。液体或低水平的观察结果是矛盾的。 ES具有非平面地形图，并研究与控制生物污染和靶向药物沉积有关的情况。