Analysis of molecular mechanism on the training effects using adrenergic receptor agonists

肾上腺素受体激动剂对训练效果的分子机制分析

• 批准号: 15500437
• 负责人: KITAURA Takashi
• 金额: $ 2.43万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500437/
• 关键词: clenbuterol; osteoclast; COX--2; bone resorptive cytokine; turobuterol; isoproterenol; UCP3; MyoD; 筋肥大; 速筋化; 乳酸トランスポーター; RT-PCR法; クロストーク; 破骨細胞誘導因子

In this study, we tried to examine the longitudinal inhibitory growth effects on bone of clenbuterol (beta-2 adrenergic receptor agonist) using reverse-transcription polymerase chain reaction (RT-PCR) analysis of the cultured osteoclast cells derived from mouse bone marrow cells with calcitriol (10 nM)and dexamethasone (10 nM). During the differentiation process from preosteoclasts into the matured osteoclasts, the clenbuterol were administered into the culture system. Clenbuterol increased the number of osteoclasts. The both of tulobuterol and isoproterenol also increased the osteoclasts. But the selective beta-2 antagonist (butoxamine) and H89 (protein kinase A inhibitor) inhibited the increased osteoclasts. It suggested the increased osteoclasts might be induced by the increased cAMP.It is well known that the PGE2 accelerate the forming of osteoclasts. The mRNA expression of COX-2 which is PGE2 synthetase also increased with the treatments of clenbuterol, turobuterol, and isoproterenol. Furthermore, the mRNA expression of bone resorptive cytokines, IL-1β and IL-6 were increased by clenbuterol, which also accelerated the bone forming by the osteoblasts activation... These results suggested that this clenbuterol affected more strongly on osteoclast forming system but a little on osteoblast. It means that these drugs have the activating action on various cells but the whole effects are complicated. Therefore, the using of these drugs should be careful.We confirmed that clenbuterol increased the expression of mRNAs of UCP3 which increases lipolysis and MyoD which is nuclear regulatory factor. They suggested that clenbuterol increased the MyoD as the myogenic master regulator and might induce the muscle hypertrophy and the transformation from slow-to fast-twitch muscle.

在这项研究中，我们试图对纵向生长杀伤对clenbuterol Anscription聚合酶链反应（RT-PCR）分析的纵向作用，对源自钙骨骨骨髓细胞（10 nm）和右肌（10 nm）（10 nm）的小鼠骨骨骨髓细胞衍生的培养的破骨细胞细胞分析（RT-PCR）分析。进行培养系统。众所周知，PGE2加速了骨细胞的形成。这也使成骨细胞的骨形成了骨骼的激活……这表明该clendodod在破骨细胞系统上更加强烈地在成骨细胞上占据这些药物要小心。我们证实了UCP3核调节因子的mRNA的表达。

项目成果

Effects of tulobuterol on skeletal muscles of mice.

妥洛特罗对小鼠骨骼肌的影响。

• 发表时间: 2003
• 期刊: Hokuriku Taiikugaku Kiyou 39
• 作者: Satoh Atsushi;Kitaura Takashi;Nakagawa Rie;Shimizu Takahiro;Ohgata Tatsuya
• 通讯作者: Ohgata Tatsuya

生化学,生理学からみた骨格筋に対するトレーニング効果[第2版]

从生物化学和生理学的角度探讨骨骼肌的训练效果[第2版]

• 发表时间: 2003
• 作者: Ohgata Tatsuya;Satoh Atsushi;Kitaura Takashi;Kraemer W.J.;山田 茂
• 通讯作者: 山田 茂

骨格筋肥大とβ_2-agonistとの関連

骨骼肌肥大与β_2激动剂的关系

• 发表时间: 2005
• 期刊: 体力科学 54・1
• 作者: 北浦 孝

Effects of clenbuterol and lactate on osteogenesis

克伦特罗和乳酸对成骨的影响

• 发表时间: 2003
• 期刊: 体力科学 52・6
• 作者: 北浦 孝;Kitaura Takashi;松本 健太郎;Kitaura Takashi;松本健太郎;佐藤 厚志;大形 辰也
• 通讯作者: 大形 辰也

Satoh, A., Ohgata, T., Kitaura, T.: "Effects of clenbuterol on the expression of MCT1 and CD147"Jap.J.Phys.Fitness Sports Med.. 52・6. 860 (2003)

Satoh, A.、Ohgata, T.、Kitaura, T.：“瘦肉精对 MCT1 和 CD147 表达的影响”Jap.J.Phys.Fitness Sports Med. 52・6 (2003)。