Evaluation of pancreatic microvasculature by contrast-enhanced endosonography with high resolution -application to diagnosis and treatment-

高分辨率对比增强超声内镜评估胰腺微血管系统-在诊断和治疗中的应用-

• 批准号: 15500353
• 负责人: KITANO Masayuki
• 金额: $ 2.3万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500353/
• 关键词: endosonography; microcirculation; contrast-enhanced harmonic mode; 超音波造影剤; 超音波内視鏡検査; 膵腫瘍

The purposes of the present study were developing an echoendoscope equipped with contrast-harmonic mode for 2^<nd> generation of contrast agents and establishing the method to observe microcirculation of digestive organs by means of it. During the period from 2003 to 2004, we succeeded to invent contrast-harmonic mode that is suite for 2^<nd> generation of contrast agents and to develop a microconvex probe available for the mode. Furthermore, we observed microcirculation of digestive organs in dogs with the probe. In 2005, we made an animal model of pancreatic carcinomas by ablating a part of the pancreas, which resulted in a hypovascular lesion, and observed the vascularity of the lesion. Those results suggested the possibility to observe microcirculation of pancreatic carcinomas. We also evaluated the clinical significance of the contrast-enhanced harmonic ultrasonography. Through our studies, it was suggested that the contrast-enhanced ultrasonography is useful for depicting pancreatic carcinomas (Gut 2004, 53, 854-859), evaluating the response of pancreatic carcinomas to chemotherapy (J Gastroenterol 2006 in press) and estimating the malignant potential of gastrointestinal stromal tumors (J Gastroenterol 2005, 40, 247-255). We reported that endosonography guided celiac plexus neurolysis required computed tomography when B mode was employed (Digestive Endoscopy 2006, in press). Because the 2^<nd> generation of contrast agents have not been permitted to use in Japan, we performed the clinical trial of contrast-enhanced harmonic endosonography at Bethesda general hospital in Germany where one of 2^<nd> generation of contrast agents, SonoVue, were permitted for clinical use. The contrast-enhanced endosonography was performed in 17 cases. It visualized the parenchymal images of pancreatic and gastrointestinal stromal tumors that previous endosonography failed to depict. This result demonstrated that contrast-enhanced endosonography was available for clinical purpose.

本研究的目的是开发一种配备第二代造影剂的对比谐波模式的回声内窥镜，并建立利用其观察消化器官微循环的方法。 2003年至2004年期间，我们成功发明了适用于第二代造影剂的对比谐波模式，并开发了适用于该模式的微凸探头。此外，我们还用探针观察了狗消化器官的微循环。 2005年，我们制作了胰腺癌动物模型，通过消融部分胰腺，形成缺血性病灶，观察病灶的血管分布。这些结果表明观察胰腺癌微循环的可能性。我们还评估了造影增强谐波超声检查的临床意义。通过我们的研究，有人建议超声造影可用于描绘胰腺癌（Gut 2004, 53, 854-859）、评估胰腺癌对化疗的反应（J Gastroenterol 2006 待出版）以及估计恶性潜能胃肠道间质瘤（J Gastroenterol 2005, 40, 247-255）。我们报道称，当采用 B 模式时，超声内镜引导的腹腔神经丛神经松解术需要计算机断层扫描（消化内镜检查 2006 年，出版中）。由于第2代造影剂在日本尚未被允许使用，我们在德国贝塞斯达综合医院进行了造影增强谐波内超声检查的临床试验，其中第2代造影剂之一， SonoVue 获准用于临床。 17例进行了造影内超声检查。它使以前的超声内镜无法描绘的胰腺和胃肠道间质瘤的实质图像可视化。该结果表明对比增强超声内镜可用于临床目的。

项目成果

Estimation of the malignant potential of gastrointestinal stromal tumors : The value of contrast-enhanced coded phase-inversion harmonic US.

胃肠道间质瘤恶性潜能的估计：对比增强编码反相谐波US的价值。

• 发表时间: 2005
• 期刊: Journal of Gastroenterology 40・3
• 作者: Fukuta N;Kitano M;Maekawa K;Chikugo T;Kudo M
• 通讯作者: Kudo M

Value of computed tomography for evaluating the injection site in endosonography guided celiac plexus neurolysis.

计算机断层扫描在内超声引导下腹腔神经丛神经松解术中评估注射部位的价值。

• 发表时间: 2006
• 期刊: Digestive Endoscopy In press
• 作者: Sakamoto H;Kitano M;Nishio T;Takeyama Y;Yasuda C;Kudo M
• 通讯作者: Kudo M

Comparison of standard-dose and low-dose regimens of gemcitabine for patients with pancreatic adenocarcinoma : a preliminary prospective randomized trail.

吉西他滨标准剂量和低剂量治疗胰腺癌患者方案的比较：初步前瞻性随机试验。

• 发表时间: 2006
• 期刊: Journal of Gastroenterology 41・1
• 作者: Sakamoto H;Kitano M;Suetomi Y;Takeyama Y;Ohyanagi H;Nakai T;Yasuda C;Kudo M
• 通讯作者: Kudo M

The vagus regulates histamine mobilization from rat stomach ECL cell by controlling their sensitivity to gastrin.

迷走神经通过控制大鼠胃 ECL 细胞对胃泌素的敏感性来调节组胺的动员。

• 发表时间: 2005
• 期刊: Journal of Physiology 564・3
• 作者: Norlen P;Ericsson P;Kitano M;Ekelund M;Hakanson R
• 通讯作者: Hakanson R

Ischemia of the rat stomach mobilizes ECL-cell histamine.

大鼠胃缺血会动员 ECL 细胞组胺。

• 发表时间: 2005
• 期刊: American Journal of Physiology 288・5
• 作者: Kitano M;Bernsand M;Kishimoto Y;Norlen P;Hakanson R;Haenuki Y;Kudo M;Hasegawa J
• 通讯作者: Hasegawa J