The function of Semaphorin on the neural network formation in the olfactory system.

信号蛋白对嗅觉系统神经网络形成的作用。

• 批准号: 15500229
• 负责人: TANIGUCHI Masahiko
• 金额: $ 2.43万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500229/
• 关键词: axon guidance; semaphorin; olfactory bulb; glomerulus; olfactory sensory map; knockout mouse

Semaphorin 3A (Sema3A) repels growing olfactory axons that express Neuropilin-1, a receptor for Sema3A. The Sema3A-mediated axon guidance seems to be essential for the formation of the glomerular sensory map in the olfactory bulb. To understand whether and how Sema3A is involved in the sensory map formation, we examined the glomerular map in the olfactory bulb of adult Sema3A-deficient mice. In the wild-type mice Neuropilin-1-positive glomeruli form the lateral and medial bands and avoid the anteromedial and ventral regions of the olfactory bulb. In the Sema3A-deficient olfactory bulb Neuropilin-1-positive glomeruli spread over the entire olfactory bulb and we consistently found the ectopic arrangement of Neuropilin-1-positive glomeruli in the anteromedial and ventral regions. In addition, a specific subset of Neuropilin-1-negative and OCAM-positive glomeruli, especially those in the anteromedial region, disappeared from the mutant olfactory bulb. These results show a critical role for Sema3A in the spatial arrangement of glomeruli in the olfactory bulb. Optical imaging from the dorsal olfactory bulb showed that the distorted glomerular map conserved molecular-feature domains. However, the positions of the domains were shifted, which suggests a secondary rearrangement of the glomerular map in the Sema3A-deficient olfactory bulb.

信号素3a（SEMA3A）驱除了表达神经蛋白-1的嗅觉轴突，即Sema3a的受体。 SEMA3A介导的轴突引导似乎对于嗅球中肾小球感觉图的形成至关重要。为了了解SEMA3A是否参与感觉图的形成，我们检查了成年SEMA3A缺陷小鼠的嗅球中的肾小球图。在野生型小鼠中，神经蛋白-1阳性肾小球形成外侧和内侧带，并避免嗅球的前内侧和腹侧区域。在SEMA3A缺陷型嗅球神经蛋白-1阳性肾小球上分布在整个嗅球上，我们一直在内部和腹侧区域发现Neuropilin-1阳性肾小球的异位排列。此外，神经蛋白-1阴性和OCAM阳性肾小球的特定子集，尤其是前中型区域的肾小球，从突变的嗅球中消失了。这些结果表明，SEMA3A在嗅球中肾小球的空间排列中至关重要。背面嗅球的光学成像表明，畸形的肾小球图保守的分子 - 特征结构域。但是，域的位置被移动，这表明在缺乏SEMA3A缺乏的嗅球中，肾小球图的次要重排。

项目成果

Vascular endothelial growth factor controls neuronal migration and cooperates

血管内皮生长因子控制神经元迁移并配合

• 发表时间: 2004
• 期刊: Genes Dev. 18
• 作者: Masahiko Taniguchi;Takao Shimizu;Quenten Schwarz et al.
• 通讯作者: Quenten Schwarz et al.

Simone P.Niclou et al.: "Meningeal cell-derived semaphorin 3A inhibits neurite outgrowth."Mol.Cell.Neurosci.. 24. 902-912 (2003)

Simone P.Niclou 等人：“脑膜细胞衍生的信号蛋白 3A 抑制神经突生长。”Mol.Cell.Neurosci.. 24. 902-912 (2003)

Masahiko Taniguchi et al.: "Distorted odor maps in the olfactory bulb of Semaphorin 3A-deficient mice."J.Neurosci. 23. 1390-1397 (2003)

Masahiko Taniguchi 等人：“Semaphorin 3A 缺陷小鼠嗅球中的气味图谱扭曲。”J.Neurosci。

Characterization of a novel member of murine semaphorin family.

• DOI: 10.1016/j.bbrc.2003.12.083
• 发表时间: 2004-01
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: M. Taniguchi;Takao Shimizu

Guido Serini et al.: "Class 3 semaphorins control vascular morphogenesis by inhibiting integrin functtion."Nature. 424. 391-397 (2003)

Guido Serini 等人：“3 类信号蛋白通过抑制整合素功能来控制血管形态发生。”《自然》。