DENDRITE-ASSOCIATED CELL ABHESION MOLECULE OBCAM IN THE HYPOTHALAMIC MAGNOCELLULAR NEURONS

下丘脑大细胞神经元中树突相关细胞吸附分子 OBCAM

• 批准号: 14580729
• 负责人: MIYATA Seiji
• 金额: $ 2.3万
• 依托单位: KYOTO INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580729/
• 关键词: dendrite; targetting; brain; neurohypophysis; plasticity; cell adhesion molecule; oxytocin; vasopressin; 接着因子; 免疫グロブリンスーパーファミリー; 可塑性; 樹状突起; 開口分泌; バソプレッシン; スパイン

Opioid-binding cell adhesion molecule (OBCAM) is one of immunoglobulin superfamily cell adhesion molecule and consists of three IgG-like domains. In the present study, it was shown that OBCAM located specifically at the dendrites of magnocellular neurons of the hypothalamic supraoptic and paraventricular nucleus. Electron microscopic observation further showed that OBCAM was localized at vasopressin-secreting neurosecretory granules of the dendrites and released together with dendritic release of vesicles. In cultured cortical neurons, OBCAM was also observed to target to their dendritic shaft specifically at postsynaptic dendritic spine. Electron microscopic observation revealed that OBCAM was seen to accumulate at postsynaptic membrane and density. Cross-linking study demonstrated that OBCAM was able to bind homophilically each other and heterophilically with Kilon, another immunoglobulin superfamily member protein.

阿片类结合细胞粘附分子（OBCAM）是免疫球蛋白超家族细胞粘附分子之一，由三个IgG样结构域组成。在本研究中，表明obcam专门位于下丘脑上脑和脑室核的巨细胞神经元的树突上。电子显微镜观察进一步表明，OBCAM定位于树突的加压素分泌神经分泌颗粒，并与囊泡的树突释放一起释放。在培养的皮质神经元中，还观察到obcam靶向其树突状轴，专门针对突触后树突状脊柱。电子显微镜观察表明，发现OBCAM在突触后膜和密度下积聚。交联研究表明，obcam能够彼此均匀地结合并与另一个免疫球蛋白超家族蛋白kilon杂质结合。

项目成果

Matsunaga et al.: "Expression of high levels of tubulin and microtubule-associated protein 2d in the neurohypophysial astrocytes of adult rat"Neuroscience. 111. 151-162 (2002)

Matsunaga 等人：“成年大鼠神经垂体星形胶质细胞中高水平微管蛋白和微管相关蛋白 2d 的表达”神经科学。

Miyata S, Matsumoto N, et al.: "Biochemical and ultrastructural analyses of IgLON cell adhesion molecules, Kilon and OBCAM in the rat brain"Neuroscience. 117. 645-658 (2003)

Miyata S、Matsumoto N 等人：“大鼠脑中 IgLON 细胞粘附分子、Kilon 和 OBCAM 的生化和超微结构分析”神经科学。

Maekawa S, Iino T, Miyata S: "Molecular characterization of the detergent-insoluble cholesterol-rich membrane microdomain (raft) of the central nervous system"Biochemica et Biophysica Acta Biomembranes. 1610. 261-270 (2003)

Maekawa S、Iino T、Miyata S：“中枢神经系统的去污剂不溶性富含胆固醇的膜微域（筏）的分子特征”《生物化学与生物物理学学报》生物膜。

Miyata S, et al.: "Polarized targetting of IgLON cell adhesion molecule OBCAM to dendrites in cultured neurons"Brain Research. 979. 123-136 (2003)

Miyata S 等人：“IgLON 细胞粘附分子 OBCAM 极化靶向培养神经元中的树突”大脑研究。

Miyata S, Taguchi K, et al.: "Dendrite-associated opioid-binding cell adhesion molecule localizes at neurosecretory granules in the hypothalamic magnocellular neurons"Neuroscience. 122. 169-181 (2003)

Miyata S、Taguchi K 等人：“树突相关的阿片类药物结合细胞粘附分子定位于下丘脑大细胞神经元的神经分泌颗粒”神经科学。