Bone regeneration therapy using HAp/Cot with MSC.

使用 HAp/Cot 和 MSC 进行骨再生治疗。

基本信息

批准号：
13557125
负责人：
SHINOMIYA Kenichi
金额：
$ 9.02万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

When we started this study, hydroxyapatite/collagen nanocomposite (HAp/Col), which is an artificial bone incorporated into bone remodeling processes, had been developed. And tissue engineering with human mesenchymal stern cells (MS Cs)had been developed for clinical use of bone reconstruction. The purpose of this study was to develop the bone regeneration system using HAp/Col combined with MSC.At the beginning of this study, there had been only a dense block of HAp/Col, and it was not suitable or using as scaffold of MSC. For this reason or others, we, tried to develop some forms of HAp/Col. Porous HAp/Col is the most desirable as a scaffold of MSC among varieties of the HAp/Col implants developed in this study. In addition, the porous body of HAp/Col has also more favorable characteristics as artificial bone than the other forms of HAp/Cot implants. The development of the porous HAp/Col is ongoing, aiming to be a commercialized product.During this study, we had to study bone formation ability of MSC/scaffold hybrids without HAp/Col because porous HAp/Col had been under development 'Porous β-TCP (commercial product) was selected as the scaffold of MSC. We confirmed the bone formation ability of human MSC, but there were significant differences of theability among donors. A clinical application model of bone regeneration with MSC was also tested using the lumbar posterolateral fusion (PLF) model of monkey. PLF is one of the most difficult model to obtain successful results because the formed bone bridging transverse processes is ectopic bone and it must have enough strength to bear mechanical stresses from various directions. The results we obtained from the monkeys' PLF using MSC/β-TCP hybrids were superior to the conventional method using autograft bone. It would indicate the possibility that the use of MSC/β-TCP hybrids in clinical application is superior to conventional autograft bone.

当我们开始这项研究时，羟基磷灰石/胶原纳米复合材料（HAp/Col）是一种融入骨重塑过程的人造骨，并且已经开发出用于临床使用的人类间充质干细胞（MS Cs）的组织工程。本研究的目的是开发HAp/Col结合MSC的骨再生系统。在本研究开始时，只有致密的HAp/Col块，并且它是骨再生系统。由于这个原因或其他原因，不适合或用作 MSC 支架，我们尝试开发某些形式的 HAp/Col。此外，HAp/Col 的多孔体还具有比其他形式的 HAp/Cot 植入物更有利的特性。多孔 HAp/Col 的开发正在进行中，旨在成为一种人工骨。在这项研究中，我们必须研究没有 HAp/Col 的 MSC/支架混合物的骨形成能力，因为多孔 HAp/Col 正在开发中。已选择多孔 β-TCP（商业产品）作为 MSC 的支架。人MSC的骨形成能力，但供体之间的能力存在显着差异，并使用MSC的腰椎后外侧融合（PLF）模型测试了MSC骨再生的临床应用模型。猴子的PLF是最难获得成功结果的模型之一，因为形成的骨桥横突是异位骨，它必须具有足够的强度来承受来自各个方向的机械应力。我们使用MSC/从猴子的PLF获得的结果。 β-TCP混合体优于使用自体移植骨的传统方法这表明MSC/β-TCP混合体在临床应用中优于传统自体移植骨的可能性。