Neurophysiological Study on Onset Mechanism of Ear Symptoms in Temporomandibular Disorders

颞下颌关节疾病耳部症状发病机制的神经生理学研究

• 批准号: 13470410
• 负责人: SASAKI Keiichi
• 金额: $ 4.61万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470410/
• 关键词: temporomandibular disorders; auditory brain stem response; Guinea pig; electrical stimulation; capsicin; 聴性脳幹反応; 耳症状; 痛覚

Ear symptoms, such as tinnitus and earache, are observed frequently in the patients of temporomandibular disorders. The aim of this study was to evaluate the influence of sensory inputs from the orofacial area on the auditory neural conduction by means of recording auditory brainstem response (ABR).Guinea-pigs were anesthetized with pentobarbital sodium (25mg/kg) intraperitoneally and used for the experiment. Animals were mounted on the stereotaxic apparatus and were inserted a earbur with a small speaker into right external auditory canal. Sonic stimulation for eliciting ABR was supplied as click sound (frequency : 9 Hz) from the speaker. Recording electrodes of ABR were attached beneath the skin at the median vertex (Cz) and the Ieft and right mastoid (Al, A2). A reference electrode was placed at occiput. Sound pressure of the sonic stimuli was 100 dB at the beginnings of the experiment and then reduced by 5 dB until all of the responses in ABR were diminished. Waves of ABR at each s … More ound pressure level were averaged 100 times and averaged waveforms of ABR were estimated.Single and train rectangle constant current pulses (duration : lmec) were applied for electrical stimulation to the orofacial regions. Subcutaneus injection of Capsicin (100mg/10 μl) was used as chemical pain stimulation. Electrical and chemical stimulation were applied to the periodontal ligaments of canine, oral mucosa and masseter muscles.Before applying stimulation, ABR was recorded as a control. ABRs were also recorded after injection of 30 μ1 of capsicin. Before stimulation, six responses ( Wave I, II, III, IV, V, VI) were obviously obserbed in recorded ABR and the latency of these responses were reduced as reducing the sound pressure. After applying the chemical stimllation, the responses of ABR were not changed. The results after applying the electrical stimulation were the same as the results of the chemical stimulation. Namely, transient electrical or chemical pain stimulation could not induce the change of ABR.These results suggest that transient noxious stimulation to the orofacial region does not cause the ear dysfunction such as ear symptoms observed in temporomandibular disorders patients. Therefore, it suggests the need of chronic model animal experiment, in which model animal with temporomandibular disorders would be made by artificial malocclusion. The relation of orofacial symptoms and ear dysfunction should be observed in thoese experiments. Less

在颞下颌疾病的患者中，经常观察到耳鸣和耳痛，例如耳鸣和耳痛。这项研究的目的是通过记录听觉的脑干反应（ABR）来评估口腔区域的感觉输入对听觉中性传导的影响。用戊巴比妥钠（25mg/kg）腹膜内麻醉Guina-Pigs，并用于腹膜内并用于实验。将动物安装在立体定位设备上，并将其带有一个小扬声器的耳朵插入右外听管中。提供用于引发ABR的声音刺激作为扬声器的单击声音（频率：9 Hz）。 ABR的记录电子在皮肤中间顶点（CZ）和IEFT和右乳突（AL，A2）下附着。将参考电极放在枕骨上。在实验的开始时，声音刺激的声压为100 dB，然后减少5 dB，直到ABR中的所有响应减少。在每个S上的ABR的波浪平均为100倍，并估算了ABR的平均波形。将单个和训练矩形恒定电流脉冲（持续时间：LMEC）应用于口面区域的电刺激。辣椒素注射辣椒素（100mg/10μL）用作化学疼痛刺激。将电刺激和化学刺激应用于犬，口腔粘膜和咬肌的牙周韧带。在施加刺激之前，将ABR记录为对照。注射30μ1辣椒素后，还记录了ABR。在刺激之前，在记录的ABR中明显观察到了六个反应（第一波，II，III，IV，V，VI），并且随着降低声压的降低，这些响应的延迟减少了。应用化学刺激后，ABR的响应不会改变。应用电刺激后的结果与化学刺激的结果相同。也就是说，瞬时电或化学疼痛刺激无法诱导ABR的变化。这些结果表明，对口面区域的短暂性有害刺激不会引起耳朵功能障碍，例如在颞下颌疾病患者中观察到的早期症状。因此，它表明需要慢性模型动物实验，其中通过人工不张紧会使具有颞下颌疾病的模型动物。在这些实验中应观察到​​口面症状和早期功能障碍的关系。较少的

项目成果

佐々木啓一, 渡辺誠: "EMGバイオフィードバック咬合診断装置(Biojaws)を用いた顎関節症の咬合診断と治療"歯界展望/増刊号 歯科医学と健康の創造. 306 (2001)

Keiichi Sasaki、Makoto Watanabe：“使用肌电图生物反馈咬合诊断装置（Biojaws）对颞下颌关节疾病进行咬合诊断和治疗”《牙科视角/特刊》，Creation of Dentistry and Health 306（2001）。

許 重人: "耳介エピテーゼ装着患者における聴力機能評価"顎顔面補綴. 24(2). 92 (2001)

Shigeto Xu：“佩戴耳廓假体患者的听力功能评估”颌面修复术24（2）92（2001）。

Ogawa T et al.: "Property of Masseter Motor Units Related to 3-D Bite Force"J Dent Res. 80(SI). 741 (2001)

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