by using DNA microarray

通过使用DNA微阵列

基本信息

批准号：
13470395
负责人：
ABIKO Yoshimitsu
金额：
$ 9.86万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

The periodontium functions as a single unit, even though each of its components has a distinct function, composition and connective tissue architecture. Thus it is important and significant to know molecular functions of each periodontal tissue cells using the genome-based research technology. In this study, we constructed the cell culture system depend on each periodontal tissue cell type, gingival epithelial, gingival fibroblastic, periodontal ligament derived, and osteoblatic cells, concerning to specific process of periodontal diseases, and analyzed a large scale of gene expression monitoring using bioinformatics technology. (1) Human gingival epithelial. cells and gingival fibroblasts were primary cultured from human normal gingival tissue, and a DNA microarray technique was used to analyze the different gene expressions. (2) Periodontal ligament derived cells were challenged with mechanical stress, periodontal pathogen LPS and Emdogain, and monitored those gene expression changes … More . (3) Osteoblasts were irradiated with low level diode laser and constructed subtractive gene library and analyzed genes enhanced transcription level, and further analyzed using DNA microarray technology. (4) Human mesenchymal bone marrow stem cells were induced to osteoblasts and monitored gene expression levels using GeneChip. (5) Human placenta trophoblasts were challenged by caffeine, and monitored gene expression levels using DNA inicroarray, further, rat placenta from caffeine feeding pregnant mouse were analyzed by RT-PCR. Effect of ageing on gene expression leve in submandibular glands of mouse was examined by DNA microarray. The reasons why we conducted these study were : periodontal diseases induced low birth infant; saliva components strongly supports health of periodontal tissues.A large scale of gene expression profiling conducted by our experimental models in each periodontal tissues cells, may let us to well understand the specific pathological process of periodontal diseases, to develop new approach of prevention of this disease, and to help the regeneration of periodontal tissues. Less

牙周化的单位，即使是艰难的组成部分，都有尤其是知道的，并且知道分子funcheodyther tistruection该系统依赖于牙周组织类型，牙龈上皮，牙周培养物和骨细胞细胞，涉及牙周疾病的特定过程，并分析了大规模的基因表达监测生物信息信息技术（1）人类牙龈上皮分析不同的基因LPS和EMDOGAIN，并监测了用Lowlaser Laser激光辐射的成骨细胞变化使用GENECHIP进行茎到成骨细胞，并使用DNA不阵列监测基因表达水平，此外，通过测试了咖啡因喂养孕妇的RT-PCR胎盘的大鼠胎盘，并通过亚细质腺体的基因表达效果检查DNA微阵列。牙周组织。