Introduction of vessels to ischemic myocardium using angiogenic growth factor : evaluation of cells on the vessel wall in 3 dimensional way

使用血管生成因子将血管引入缺血心肌：以3维方式评估血管壁上的细胞

• 批准号: 13470279
• 负责人: ENDO Masahiro
• 金额: $ 3.2万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470279/
• 关键词: CABG; coronary revascularization; angiogenesis; bFGF; scaffold; tissue engineering; sprout; vessel; 血管壁; 血管新生; 線維芽細胞増殖因子; 足場(scaffold); 組織再構築; 血管誘導; RECモデル; ウサギ; angiogenesis; coronary; 虚血; 心筋; 三次元; 壁細胞

For patients who can not have surgical revascularization because of atheromatous small coronary artery in severe ischemic heart disease, therapeutic angiogenesis is demanded. For angiogenesis, combination of cells, extracellular matrix and growth factor is important. However, in clinic, TMLR and angiogenic factors have not been satisfactory. In therapeutic angiogenesis, it is difficult to distinguish newly formed vessels from existing ones in the heart because there are so may capillaries, such as four capillaries for one cardiomyo-bundle. In this research period, we planned 1)evaluate the process of angiogenesis from sprout formation to mature vessels using angiogenic factor and scaffold, 2)reconstruction of vessel image into 3-dimentional to understand cells in the vessel wall. In a newly formed vessel, sprout formation occur from venous side. Spouts have one layer of cells, endothelium. However, at pint of differentiation, vessels become artery and vein was not recognized. However, direct observation of angiogenesis in rabbits was possible.

对于严重缺血性心脏病中因小冠状动脉粥样硬化而无法进行血运重建的患者，需要治疗性血管生成。对于血管生成，细胞、细胞外基质和生长因子的结合很重要。然而，在临床上，TMLR和血管生成因子的研究还不尽如人意。在治疗性血管生成中，很难将心脏中新形成的血管与现有血管区分开来，因为存在如此多的毛细血管，例如一个心肌束有四个毛细血管。在本研究期间，我们计划1）利用血管生成因子和支架评估从芽形成到成熟血管的血管生成过程，2）将血管图像重建为3维以了解血管壁中的细胞。在新形成的容器中，芽的形成是从静脉侧发生的。喷口有一层细胞，即内皮。然而，在分化阶段，血管变成了动脉，而静脉则未被识别。然而，直接观察兔子的血管生成是可能的。

项目成果

Endo M, Tomizawa Y, Nishida H.: "Transmyocardial laser revascularization (TMR)"Nippon Rinsho. 61 Suppl 4. 704-706 (2003)

Endo M、Tomizawa Y、Nishida H.：“经心肌激光血运重建（TMR）”Nippon Rinsho。

Endo M et al.: "Benefit of bilateral over single internal mammary artery grafts for multiple coronary artery bypass grafting"Circulation. 104. 2164-2170 (2001)

Endo M 等人：“双侧乳内动脉移植相对于单侧乳内动脉移植对于多冠状动脉旁路移植术的益处”循环。

Tomizawa Y. et al.: "Macroscopic sequential pictures of angiogenesis in a rabbit ear chamber"J Invest Surg. 15. 269-274 (2002)

Tomizawa Y. 等人：“兔耳室血管生成的宏观连续图片”J Invest Surg。

遠藤真弘, 富澤康子: "CABGのスタンダード、先端外科医療の最前線"医学のあゆみ. 205. 678-682 (2003)

Masahiro Endo、Yasuko Tomizawa：“CABG标准，先进外科医学的最前沿”医学史205。678-682（2003）。

遠藤真弘, 冨澤康子 他: "血管新生療法(レーザー心筋血行再建術)、IV.血管新生・再建療法"日本臨床. 61増刊. 704-706 (2003)

Masahiro Endo、Yasuko Tomizawa 等人：“血管生成治疗（激光心肌血运重建），IV.血管生成/重建治疗”日本临床 61 特别版（2003 年）。