Establishment of a method to induce cardiomyocyte differetiation from bone marrow mesenchymal stem cell

骨髓间充质干细胞诱导心肌细胞分化方法的建立

• 批准号: 13470151
• 负责人: FUKUDA Keiichi
• 金额: $ 10.5万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470151/
• 关键词: cardiomyocytes; Stem cell; Bone Marrow mesenchymal stem cell; Cell transplantation therapy; Differentiation; CD antigen; Regenerative medicine; 骨髄; 表面抗原; FACS; 多分化能; 多能性幹細胞; 心不全; 発生分化; 間葉系幹細胞; 細胞増殖因子; サイトカイン

In order to obtain regenerative cardiomyocyte from bone marrow, we had developed a method to isolate mesenchymal stem cell from bone marrow. Using autoMACS, CD45-, CD29+, CD34-, CD44+ cells were isolated from fresh whole bone marrow cells, and were seeded on the fibronectin-coated dishes. FACS analysis revealed that these cells also showed CD 13-, and CD49e+. These cells could proliferate in culture, and differentiated into the adipocytes under the medium containing insulin. Moreover, these cells also differentiated into osteoblast under the condition of dexamethazone, ascorbic acid, and β-glyceroposphates. IGF-1, FGF, TGF-b, LIF, EGF, nor endotelin-1 did not augment cardimoyocyte differentiation in these cells. However, Wnt11 augmented cardiomyocyte differentiation in these cells.

为了从骨髓中获得再生性心肌细胞，我们开发了一种与骨髓分离出肾小球茎的方法。 FABRONECTIN涂层的盘子分析显示了CD 13-和CD49E+。 IGF-1，FGF，TGF，LIF，EGF和NENTOLIN-1没有增强这些细胞中的Cardimoyoyoypyte离子。

项目成果

Eiichi Takahashi, Keiichi Fukuda, et al.: "LIF activates cardiac L-type Ca^<2+> channels via phosphorylation of serine 1829 of rabbit Cav1.2"Cire Res. 94. 1242-1248 (2004)

Eiichi Takahashi、Keiichi Fukuda 等人：“LIF 通过兔 Cav1.2 丝氨酸 1829 的磷酸化激活心脏 L 型 Ca^2 通道”Cire Res。

Keiichi Fukuda: "Molecular characterization of regenerated cardiomyocytes derived from adult mesenchymal stem cells."Congenital anomalies. 42. 1-9 (2002)

Keiichi Fukuda：“源自成体间充质干细胞的再生心肌细胞的分子特征。”先天性异常。

Keiichi Fukuda: "Stem cell transplantation as a mode of regenerative medicine."Jpn Med Association J.. 46. 333-338 (2003)

Keiichi Fukuda：“干细胞移植作为再生医学的一种模式。”Jpn Med Association J.. 46. 333-338 (2003)

keiichi Fukuda: "Molecular characterization of regenerated cardiomyocytes derived from adult mesenchymal stem cells"Congenital anomaltes. 42. 1-9 (2002)

福田敬一：“成体间充质干细胞再生心肌细胞的分子特征”先天性异常。

Keiichi Fukuda: "Regenerative medicine for cardiomyocyte."Jap Med Ass J.. 46. 333-338 (2003)

Keiichi Fukuda：“心肌细胞的再生医学。”Jap Med Ass J.. 46. 333-338 (2003)