Genetic differentiation and phylogeny of domestic and wild bovines basedon DNA information

基于DNA信息的家养牛和野生牛的遗传分化和系统发育

基本信息

批准号：
13460146
负责人：
NAMIKAWA Takao
金额：
$ 9.79万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460146/
关键词：
genetic resources animal history cattle water buffalo domestic animal genetic variation genetic differentiation satellite DNA 牛亜科牛水牛遺伝的分化系統進化多元起源説 AFLP

项目摘要

1.It has been unclosed by our group and other researchers that most Asian native cattle are originated from various grades of mixtures with three distinct types of cattle, that is, the humpless (or European type) cattle, Indian zebu cattle and banteng(Bos javanicus, a wild cattle endemic to Southeast Asia). Similarly, the domestic water buffaloes are classified into two major types, that is, the, swamp type buffaloes and river type buffaloes. In some area of eastern Asia, however there are mixed populations with the two types. The native domestic cattle and water buffaloes have the polygenic origin of domestication.2.The present research aimed to establish an efficient method to estimate genetic conformation of Asian domestic bovines by using satellite DNAs which occupy 4% of the whole genome. We analyzed repeated sequence of satellite DNAs of water buffalo and found two different repeated sequences in the satellite DNAs. One named S-I was found by a restriction enzyme BamHI and 1400bp in size of the repeat-unit (800bp and 600bp when used a restriction enzyme Stu I). The other repeat-unit named S-II was 700bp by Bam HI and 400bp and 300bp by Stu I. The S-I and S-II were confirmed to localize specific to C-band regions or the centromeric regions of the chromosomes by FISH method. However, FISH signal intensity on respective chromosomes were different between S-I and S-II. Homologies of S-I andS-II from the water buffaloes were 79% and 81% to those of cattle, respectively.3.Thus, if we could select specific restriction enzymes to produce specific fragments of those repeat-units in satellite DNAs to different types of bovines or bubalines, their genetic confirmation would be easily estimated at a level of individual. We could give essential information to establish such method.

1.我们的小组和其他研究人员未封闭，大多数亚洲本地牛都来自各种与三种不同类型的牛的混合物，即Humpless（或欧洲型）牛，印度Zebu牛和班坦（Bos Javanicus，东南亚的野生牛）。同样，家庭水牛分为两种主要类型，即沼泽型水牛和河流型水牛。在东亚的某些地区，两种类型的种群混合在一起。本地的家养牛和水牛具有驯化的多基因源。2。目前的研究旨在通过使用占据整个基因组的4％的卫星DNA来建立一种有效的方法来估计亚洲家用牛的遗传构象。我们分析了水牛的卫星DNA的重复序列，并在卫星DNA中发现了两个不同的重复序列。通过限制酶BAMHI和1400bp的重复单位（800bp和600bp）发现了一个名为S-I（使用限制酶Stu I时）。 BAM HI和400bp的另一个重复单位为700bp，STU I为300bp。确认S-I和S-II通过鱼类方法定位于C波段区域或染色体的中心区域的特定定位。但是，S-I和S-II之间的鱼信号强度在各个染色体上有所不同。水牛的S-I和II的同源性分别为牛的同源物为79％和81％。3。如果我们可以选择特定的限制酶来产生卫星DNA中的这些重复性功能的特定片段，以对不同类型的牛或泡泡糖确认，他们的遗传确认很容易在单个级别的级别上估计。我们可以提供基本信息来建立这种方法。