The speculation of the methods how to manage the wildlife in the forests around the city.

关于如何管理城市周围森林中的野生动物的方法的猜测。

基本信息

批准号：
13460062
负责人：
AOI Toshiki
金额：
$ 7.42万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2004
项目状态：
已结题

项目摘要

We mainly investigated the habitat use, nomerange, food habits, parasites, parapoxvirus for Japanese serows in the forest aloud the city. We captured one mail subadult serow near the Takizawa Experimantal Forest of Iwate University in May 2003. We attached telemetry system on the serow and chased it more than one year. Though he stayd his mother's range until February 2004, and then he suddenly moved about 1.5km downstream of Kitakami river, and established new homerange in the forest along the river. By the fact, we think the forests along the river are important as habitats for the wildlives around the city. We also analyzed the distribution for the feces piles of Japanese serow in Takizawa Experimental forest to demonstrate the human impact to select the habitat by Japanese serows in urban forests. We manipulated all the feces distribution data by geographic information system (Software Arc View 3.2, ESRI) ; computed the distance of the piles from residential areas, streets, and rai … More lroads and generated the distribution schemas. During the period of the survey, we found 119 feces piles in total, and the locations were characterized by the following. Regarding the characteristics of canopy vegetation, the serows preferred the coniferous needle-leaved tree forests such as cedar to broad-leaved forests and red pine forests. They tend to avoid places within 200 m from residential areas and streets. Surprisingly, the distance from the railroads had less effect on number of feces piles. Thus, we conclude that serows are not fond of direct contact with people, although they inhabit places near the habitat of people.Variation of two locus (albumin and transferin) was found by genetical examination of serum protein polymorphism. Also, we made a usuable antibody for a judgment of serum transferin. In addition, it is suggested that male and female distinction of Japanese serow is possible by DNA provided from feces. But its improvement is necessary, it seems that we can use it as tool of field study of Japanese serow. We performed the biochemical test of Japanese serow's blood, but an effort which we increase the number of examples in future, and to pile up basics data is necessary. To survey infection of parapoxvirus and orfvirus in Japanese serow, propagation of orfvirus was carried out in primary bovine testis cell cultures and established bovine cell culture. The virus propagated in both cell cultures but the infectivity titers were not enough to prepare viral antigen for serological tests. Further investigations for virus passages and cloning have been doing. By parasitic survey for the Japanese serows inhabiting Iwate Prefecture, 2 species of mites and 5 species of ticks were obtained from their skin and 21 species of endoparasites (13 of helminths and 8 of protozoan parasites) were also detected in the various internal organs. C.crispus living in this area may be important as reservoir host of some pathogenic parasites of domestic animals and humans Less

我们主要调查了栖息地的使用，Nomerange，粮食习惯，寄生虫，旁波病毒，用于城市森林中的日本神灵。我们于2003年5月在伊瓦特大学高泽实验森林附近捕获了一个邮件，亚族人的血清。我们将遥测系统附加在erlow上，并对其进行了超过一年的挑战。尽管他一直保持着母亲的范围，直到2004年2月，然后他突然在基塔卡米河（Kitakami River）下游约1.5公里处移动，并在河沿岸的森林中建立了新的势利。事实上，我们认为河沿岸的森林很重要，作为城市周围野生动物的栖息地。我们还分析了高泽实验森林中粪便堆的日本神灵堆的分布，以证明人类的影响，以选择日本神灵在城市森林中的栖息地。我们通过地理信息系统（Software Arc View 3.2，ESRI）操纵了所有FECES分销数据，计算了钢琴与居民区域，街道和RAI的距离，并产生了分布模式。在调查期间，我们总共发现了119枚粪便钢琴，并且位置的特征是以下。关于冠层植被的特征，梅龙更喜欢针叶叶树林（例如雪松林）而不是阔叶林和红松树林。他们倾向于避开居民区域和街道200 m以内的地方。令人惊讶的是，与铁路的距离对粪便钢琴数量的影响较小。这就是我们包含的，尽管他们居住在人的习惯附近，但ersows不喜欢与人的直接接触。通过遗传检查血清蛋白多态性的遗传检查，发现了两个基因座（白蛋白和转移素）。此外，我们制作了一种可用抗体的抗体，以判断血清转移素。另外，建议通过粪便提供的DNA可以对日本血清的男性和女性区别。但是它的改进是必要的，似乎我们可以将其用作日本血清现场研究的工具。我们对日本血液的血液进行了生化测试，但是我们将来增加了示例数量的努力，并且需要堆积基本数据。为了调查日本血清中副细胞病毒和Orfvirus的感染，在原发性牛睾丸细胞培养物中进行了Orfvirus的传播，并建立了牛细胞培养。在两个细胞培养物中均传播的病毒，但感染滴度不足以为血清学检查制备病毒抗原。对病毒通道和克隆的进一步研究一直在做。通过对遗传IWATE县的日本伴侣的寄生调查，还从其皮肤中获得了2种螨虫和5种tick虫，并在各种内部器官中也检测到21种内寄生虫（13种胚芽和8个原生动物寄生虫）。 C. C. Crispus居住在该地区可能很重要，因为家畜和人类的一些致病寄生虫的储存量很少